Gene - combatTB X

aac

Type: protein_coding
Name: aac
Locus Name: Rv0262c
Product: Aminoglycoside 2'-N-acetyltransferase Aac (Aac(2')-IC)
Functional Category: Virulence, detoxification, adaptation
Location: 314309..314854 (- strand)
Gene Length: 545 bp
Nucleotides: GTGCACACCCAGGTACACACGGCCCGCCTGGTCCACACCGCCGATCTTGACAGCGAGACCCGCCAGGACATCCGTCAGATGGTCACCGGCGCGTTTGCCGGTGACTTCACCGAGACCGACTGGGAGCACACGCTGGGTGGGATGCACGCCCTGATCTGGCATCACGGGGCGATCATCGCGCATGCCGCGGTGATCCAGCGGCGACTGATCTACCGCGGCAACGCGCTGCGCTGCGGGTACGTCGAAGGCGTTGCGGTGCGGGCGGACTGGCGGGGCCAACGCCTGGTGAGCGCGCTGTTGGACGCCGTCGAGCAGGTGATGCGCGGCGCTTACCAGCTCGGAGCGCTCAGTTCCTCGGCGCGGGCCCGCAGACTGTACGCCTCACGCGGCTGGCTGCCCTGGCACGGCCCGACATCGGTACTGGCACCAACCGGTCCAGTCCGTACACCCGATGACGACGGAACGGTGTTCGTCCTGCCCATCGACATCAGCCTGGACACCTCGGCGGAGCTGATGTGCGATTGGCGCGCGGGCGACGTCTGGTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

May catalyze the coenzyme A-dependent acetylation of the 2' hydroxyl or amino group of a broad spectrum of aminoglycosides and confer resistance to aminoglycosides (By similarity). In vitro assays show no significant increase of resistance to aminoglycosides, possibly due to low expression in a heterologous system (PubMed:9159528). {ECO:0000250|UniProtKB:P94968, ECO:0000269|PubMed:9159528}.

Family

AAC(2')-I acetyltransferase family

GO

response to antibiotic GO:0046677 biological_process
aminoglycoside N-acetyltransferase activity GO:0034069 molecular_function
aminoglycoside antibiotic catabolic process GO:0030649 biological_process
aminoglycoside 2'-N-acetyltransferase activity GO:0047921 molecular_function
plasma membrane GO:0005886 cellular_component

InterPro

UniProt: P9WQG9
GenBank: Rv0262c
EnsemblBacteria: Rv0262c
Mycobrowser: Rv0262c

Summary

Name: Aminoglycoside 2'-N-acetyltransferase (EC 2.3.1.-) (AAC(2')-Ic)
Family: AAC(2')-I acetyltransferase family
Protein Sequence: MHTQVHTARLVHTADLDSETRQDIRQMVTGAFAGDFTETDWEHTLGGMHALIWHHGAIIAHAAVIQRRLIYRGNALRCGYVEGVAVRADWRGQRLVSALLDAVEQVMRGAYQLGALSSSARARRLYASRGWLPWHGPTSVLAPTGPVRTPDDDGTVFVLPIDISLDTSAELMCDWRAGDVW
Mass: 20,038 Da
Length: 181 Aa

Ribostamycin DB03615: 5-Amino-2-Aminomethyl-6-[4,6-Diamino-2-(3,4-Dihydroxy-5-Hydroxymethyl-Tetrahydro-Furan-2-Yloxy)-3-Hydroxy-Cyclohexyloxy]-Tetrahydro-Pyran-3,4-Diol | Ribastamin | RIO | Vistamycin | Xylostacin | Xylostasin

Rv0262c doesn't seem to be involved in any pathway.

Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates.: Nat Struct Biol. 2002 Sep;9(9):653-8. doi: 10.1038/nsb830.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Aminoglycoside 2'-N-acetyltransferase genes are universally present in mycobacteria: characterization of the aac(2')-Ic gene from Mycobacterium tuberculosis and the aac(2')-Id gene from Mycobacterium smegmatis.: Mol Microbiol. 1997 Apr;24(2):431-41.