Gene - combatTB X

iniA

Type: protein_coding
Name: iniA
Locus Name: Rv0342
Product: Isoniazid inductible gene protein IniA
Functional Category: Cell wall and cell processes
Location: 410838..412760 (+ strand)
Gene Length: 1922 bp
Nucleotides: TGGTCCCCGCCGGTTTGTGCGCATACCGTGATCTGAGGCGTAAACGAGCGAGAAAGTGGGGCGACACGGTGACCCAGCCCGATGACCCACGTCGGGTCGGTGTGATCGTCGAACTGATCGATCACACTATCGCCATCGCCAAACTGAACGAGCGTGGTGATCTAGTACAGCGGTTGACGCGGGCTCGCCAGCGGATCACCGACCCGCAGGTCCGTGTGGTGATCGCCGGGCTGCTCAAACAGGGCAAGAGTCAATTGCTCAATTCGTTGCTCAACCTGCCCGCGGCGCGAGTAGGCGATGACGAGGCCACCGTGGTGATCACCGTCGTAAGCTACAGCGCCCAACCGTCGGCCCGGCTTGTGCTGGCCGCCGGGCCCGACGGGACAACCGCAGCGGTTGACATTCCCGTCGATGACATCAGCACCGATGTGCGTCGGGCTCCGCACGCCGGTGGCCGCGAGGTGTTGCGGGTCGAGGTCGGCGCGCCCAGCCCGCTGCTGCGGGGCGGGCTGGCGTTTATCGATACTCCGGGTGTGGGCGGCCTCGGACAGCCCCACCTGTCGGCGACGCTGGGGCTGCTACCCGAGGCCGATGCCGTCTTGGTGGTCAGCGACACCAGCCAGGAATTCACCGAACCCGAGATGTGGTTCGTGCGGCAGGCCCACCAGATCTGTCCGGTCGGGGCGGTCGTGGCCACCAAGACCGACCTGTATCCGCGCTGGCGGGAGATCGTCAATGCCAATGCAGCACATCTGCAGCGGGCCCGGGTTCCGATGCCGATCATCGCAGTCTCATCACTGTTGCGCAGCCACGCGGTCACGCTTAACGACAAAGAGCTCAACGAAGAGTCCAACTTTCCGGCGATCGTCAAGTTTCTCAGCGAGCAGGTGCTTTCCCGCGCGACGGAGCGAGTGCGTGCTGGGGTACTCGGCGAAATACGTTCGGCAACAGAGCAATTGGCGGTGTCTCTAGGTTCCGAACTATCGGTGGTCAACGACCCGAACCTCCGTGACCGACTTGCTTCGGATTTGGAGCGGCGCAAACGGGAAGCCCAGCAGGCGGTGCAACAGACAGCGCTGTGGCAGCAGGTGCTGGGCGACGGGTTCAACGACCTGACTGCTGACGTGGACCACGACCTACGAACCCGCTTCCGCACCGTCACCGAAGACGCCGAGCGCCAGATCGACTCCTGTGACCCGACTGCGCATTGGGCCGAGATTGGCAACGACGTCGAGAATGCGATCGCCACAGCGGTCGGCGACAACTTCGTGTGGGCATACCAGCGTTCCGAAGCGTTGGCCGACGACGTCGCTCGCTCCTTTGCCGACGCGGGGTTGGACTCGGTCCTGTCAGCAGAGCTGAGCCCCCACGTCATGGGCACCGACTTCGGCCGGCTCAAAGCGCTGGGCCGGATGGAATCGAAACCGCTGCGCCGGGGCCATAAAATGATTATCGGCATGCGGGGTTCCTATGGCGGCGTGGTCATGATTGGCATGCTGTCGTCGGTGGTCGGACTTGGGTTGTTCAACCCGCTATCGGTGGGGGCCGGGTTGATCCTCGGCCGGATGGCATATAAAGAGGACAAACAAAACCGGTTGCTGCGGGTGCGCAGCGAGGCCAAGGCCAATGTGCGGCGCTTCGTCGACGACATTTCGTTCGTCGTCAGCAAACAATCACGGGATCGGCTCAAGATGATCCAGCGTCTGCTGCGCGACCACTACCGCGAGATCGCCGAAGAGATCACCCGGTCGCTCACCGAGTCCCTGCAGGCGACCATCGCGGCGGCGCAGGTGGCGGAAACCGAGCGGGACAATCGAATTCGGGAACTTCAGCGGCAATTGGGTATCCTGAGCCAGGTCAACGACAACCTTGCCGGCTTGGAGCCAACCTTGACGCCCCGGGCGAGCTTGGGACGAGCGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Participates in the development of tolerance to both isoniazid and ethambutol. May function through a MDR-pump like mechanism, although it does not appear to directly transport isoniazid from the cell. {ECO:0000269|PubMed:15752203}.

Family

Unknown

GO

response to antibiotic GO:0046677 biological_process
cell wall GO:0005618 cellular_component
efflux transmembrane transporter activity GO:0015562 molecular_function
plasma membrane GO:0005886 cellular_component
integral component of membrane GO:0016021 cellular_component

InterPro

UniProt: P9WJ99
GenBank: Rv0342
EnsemblBacteria: Rv0342
Mycobrowser: Rv0342

Summary

Name: Isoniazid-induced protein IniA
Family: Unknown
Protein Sequence: MVPAGLCAYRDLRRKRARKWGDTVTQPDDPRRVGVIVELIDHTIAIAKLNERGDLVQRLTRARQRITDPQVRVVIAGLLKQGKSQLLNSLLNLPAARVGDDEATVVITVVSYSAQPSARLVLAAGPDGTTAAVDIPVDDISTDVRRAPHAGGREVLRVEVGAPSPLLRGGLAFIDTPGVGGLGQPHLSATLGLLPEADAVLVVSDTSQEFTEPEMWFVRQAHQICPVGAVVATKTDLYPRWREIVNANAAHLQRARVPMPIIAVSSLLRSHAVTLNDKELNEESNFPAIVKFLSEQVLSRATERVRAGVLGEIRSATEQLAVSLGSELSVVNDPNLRDRLASDLERRKREAQQAVQQTALWQQVLGDGFNDLTADVDHDLRTRFRTVTEDAERQIDSCDPTAHWAEIGNDVENAIATAVGDNFVWAYQRSEALADDVARSFADAGLDSVLSAELSPHVMGTDFGRLKALGRMESKPLRRGHKMIIGMRGSYGGVVMIGMLSSVVGLGLFNPLSVGAGLILGRMAYKEDKQNRLLRVRSEAKANVRRFVDDISFVVSKQSRDRLKMIQRLLRDHYREIAEEITRSLTESLQATIAAAQVAETERDNRIRELQRQLGILSQVNDNLAGLEPTLTPRASLGRA
Mass: 70,083 Da
Length: 640 Aa

Rv0342 doesn't seem to be a targeted by any drug.

Rv0342 doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Transcriptional regulation of multi-drug tolerance and antibiotic-induced responses by the histone-like protein Lsr2 in M. tuberculosis.: PLoS Pathog. 2007 Jun;3(6):e87. doi: 10.1371/journal.ppat.0030087.
The Mycobacterium tuberculosis iniA gene is essential for activity of an efflux pump that confers drug tolerance to both isoniazid and ethambutol.: Mol Microbiol. 2005 Mar;55(6):1829-40. doi: 10.1111/j.1365-2958.2005.04510.x.
Characterization of the Mycobacterium tuberculosis iniBAC promoter, a promoter that responds to cell wall biosynthesis inhibition.: J Bacteriol. 2000 Apr;182(7):1802-11. doi: 10.1128/jb.182.7.1802-1811.2000.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.