Gene - combatTB X

dnaK

Type: protein_coding
Name: dnaK
Locus Name: Rv0350
Product: Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70)
Functional Category: Virulence, detoxification, adaptation
Location: 419835..421712 (+ strand)
Gene Length: 1877 bp
Nucleotides: TGGCTCGTGCGGTCGGGATCGACCTCGGGACCACCAACTCCGTCGTCTCGGTTCTGGAAGGTGGCGACCCGGTCGTCGTCGCCAACTCCGAGGGCTCCAGGACCACCCCGTCAATTGTCGCGTTCGCCCGCAACGGTGAGGTGCTGGTCGGCCAGCCCGCCAAGAACCAGGCAGTGACCAACGTCGATCGCACCGTGCGCTCGGTCAAGCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAAGAAATACACCGCGCCGGAGATCAGCGCCCGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGCCTACCTCGGTGAGGACATTACCGACGCGGTTATCACGACGCCCGCCTACTTCAATGACGCCCAGCGTCAGGCCACCAAGGACGCCGGCCAGATCGCCGGCCTCAACGTGCTGCGGATCGTCAACGAGCCGACCGCGGCCGCGCTGGCCTACGGCCTCGACAAGGGCGAGAAGGAGCAGCGAATCCTGGTCTTCGACTTGGGTGGTGGCACTTTCGACGTTTCCCTGCTGGAGATCGGCGAGGGTGTGGTTGAGGTCCGTGCCACTTCGGGTGACAACCACCTCGGCGGCGACGACTGGGACCAGCGGGTCGTCGATTGGCTGGTGGACAAGTTCAAGGGCACCAGCGGCATCGATCTGACCAAGGACAAGATGGCGATGCAGCGGCTGCGGGAAGCCGCCGAGAAGGCAAAGATCGAGCTGAGTTCGAGTCAGTCCACCTCGATCAACCTGCCCTACATCACCGTCGACGCCGACAAGAACCCGTTGTTCTTAGACGAGCAGCTGACCCGCGCGGAGTTCCAACGGATCACTCAGGACCTGCTGGACCGCACTCGCAAGCCGTTCCAGTCGGTGATCGCTGACACCGGCATTTCGGTGTCGGAGATCGATCACGTTGTGCTCGTGGGTGGTTCGACCCGGATGCCCGCGGTGACCGATCTGGTCAAGGAACTCACCGGCGGCAAGGAACCCAACAAGGGCGTCAACCCCGATGAGGTTGTCGCGGTGGGAGCCGCTCTGCAGGCCGGCGTCCTCAAGGGCGAGGTGAAAGACGTTCTGCTGCTTGATGTTACCCCGCTGAGCCTGGGTATCGAGACCAAGGGCGGGGTGATGACCAGGCTCATCGAGCGCAACACCACGATCCCCACCAAGCGGTCGGAGACTTTCACCACCGCCGACGACAACCAACCGTCGGTGCAGATCCAGGTCTATCAGGGGGAGCGTGAGATCGCCGCGCACAACAAGTTGCTCGGGTCCTTCGAGCTGACCGGCATCCCGCCGGCGCCGCGGGGGATTCCGCAGATCGAGGTCACTTTCGACATCGACGCCAACGGCATTGTGCACGTCACCGCCAAGGACAAGGGCACCGGCAAGGAGAACACGATCCGAATCCAGGAAGGCTCGGGCCTGTCCAAGGAAGACATTGACCGCATGATCAAGGACGCCGAAGCGCACGCCGAGGAGGATCGCAAGCGTCGCGAGGAGGCCGATGTTCGTAATCAAGCCGAGACATTGGTCTACCAGACGGAGAAGTTCGTCAAAGAACAGCGTGAGGCCGAGGGTGGTTCGAAGGTACCTGAAGACACGCTGAACAAGGTTGATGCCGCGGTGGCGGAAGCGAAGGCGGCACTTGGCGGATCGGATATTTCGGCCATCAAGTCGGCGATGGAGAAGCTGGGCCAGGAGTCGCAGGCTCTGGGGCAAGCGATCTACGAAGCAGCTCAGGCTGCGTCACAGGCCACTGGCGCTGCCCACCCCGGCGGCGAGCCGGGCGGTGCCCACCCCGGCTCGGCTGATGACGTTGTGGACGCGGAGGTGGTCGACGACGGCCGGGAGGCCAAGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.; FUNCTION: Recombinant extracellular protein activates expression of NF-kappa-B in immortalized human dermal endothelial cells in a TLR2- and TLR4-dependent manner. Activation occurs via MYD88-dependent and -independent pathways and requires TIRAP, TRIF and TRAM (some experiments done in mouse cells, mice do not usually catch tuberculosis) (PubMed:15809303). {ECO:0000269|PubMed:15809303}.

Family

Heat shock protein 70 family

GO

cell surface GO:0009986 cellular_component
response to heat GO:0009408 biological_process
cellular response to superoxide GO:0071451 biological_process
protein autophosphorylation GO:0046777 biological_process
protein folding chaperone GO:0044183 molecular_function
positive regulation of plasminogen activation GO:0010756 biological_process
response to copper ion GO:0046688 biological_process
response to antibiotic GO:0046677 biological_process
interaction with host via substance in symbiont surface GO:0044044 biological_process
cellular response to heat GO:0034605 biological_process
cellular response to unfolded protein GO:0034620 biological_process
bacterial extracellular vesicle GO:0097691 cellular_component
positive regulation of transcription regulatory region DNA binding GO:2000679 biological_process
heat shock protein binding GO:0031072 molecular_function
protein refolding GO:0042026 biological_process
cell wall GO:0005618 cellular_component
capsule GO:0042603 cellular_component
extracellular region GO:0005576 cellular_component
ATP binding GO:0005524 molecular_function
cytosol GO:0005829 cellular_component
ATPase activity, coupled GO:0042623 molecular_function
plasma membrane GO:0005886 cellular_component
cytoplasm GO:0005737 cellular_component
fibronectin binding GO:0001968 molecular_function
zymogen binding GO:0035375 molecular_function
ATPase activity GO:0016887 molecular_function
misfolded protein binding GO:0051787 molecular_function
response to unfolded protein GO:0006986 biological_process
unfolded protein binding GO:0051082 molecular_function
chaperone cofactor-dependent protein refolding GO:0051085 biological_process

InterPro

UniProt: P9WMJ9
GenBank: Rv0350
EnsemblBacteria: Rv0350
Mycobrowser: Rv0350

Summary

Name: Chaperone protein DnaK (HSP70) (Heat shock 70 kDa protein) (Heat shock protein 70)
Family: Heat shock protein 70 family
Protein Sequence: MARAVGIDLGTTNSVVSVLEGGDPVVVANSEGSRTTPSIVAFARNGEVLVGQPAKNQAVTNVDRTVRSVKRHMGSDWSIEIDGKKYTAPEISARILMKLKRDAEAYLGEDITDAVITTPAYFNDAQRQATKDAGQIAGLNVLRIVNEPTAAALAYGLDKGEKEQRILVFDLGGGTFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVDWLVDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRAEFQRITQDLLDRTRKPFQSVIADTGISVSEIDHVVLVGGSTRMPAVTDLVKELTGGKEPNKGVNPDEVVAVGAALQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTRLIERNTTIPTKRSETFTTADDNQPSVQIQVYQGEREIAAHNKLLGSFELTGIPPAPRGIPQIEVTFDIDANGIVHVTAKDKGTGKENTIRIQEGSGLSKEDIDRMIKDAEAHAEEDRKRREEADVRNQAETLVYQTEKFVKEQREAEGGSKVPEDTLNKVDAAVAEAKAALGGSDISAIKSAMEKLGQESQALGQAIYEAAQAASQATGAAHPGGEPGGAHPGSADDVVDAEVVDDGREAK
Mass: 66,831 Da
Length: 625 Aa

Rv0350 doesn't seem to be a targeted by any drug.

Tuberculosis mtu05152
mtu05152
Human Diseases; Infectious disease: bacterial

RNA degradation mtu03018
mtu03018
Genetic Information Processing; Folding, sorting and degradation

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Mycobacteria release active membrane vesicles that modulate immune responses in a TLR2-dependent manner in mice.: J Clin Invest. 2011 Apr;121(4):1471-83. doi: 10.1172/JCI44261.
Mycobacterium tuberculosis Cpn60.2 and DnaK are located on the bacterial surface, where Cpn60.2 facilitates efficient bacterial association with macrophages.: Infect Immun. 2009 Aug;77(8):3389-401. doi: 10.1128/IAI.00143-09. Epub 2009 May 26.
Mycobacterium tuberculosis heat shock proteins use diverse Toll-like receptor pathways to activate pro-inflammatory signals.: J Biol Chem. 2005 Jun 3;280(22):20961-7. doi: 10.1074/jbc.M411379200. Epub 2005 Apr 4.
The alternative sigma factor SigH regulates major components of oxidative and heat stress responses in Mycobacterium tuberculosis.: J Bacteriol. 2001 Oct;183(20):6119-25. doi: 10.1128/JB.183.20.6119-6125.2001.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.