Gene - combatTB X

clpB

Type: protein_coding
Name: clpB
Locus Name: Rv0384c
Product: Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1)
Functional Category: Virulence, detoxification, adaptation
Location: 459456..462002 (- strand)
Gene Length: 2546 bp
Nucleotides: GTGGACTCGTTTAACCCGACGACCAAGACGCAGGCGGCGCTAACCGCGGCGTTACAGGCGGCTTCGACCGCCGGCAATCCCGAGATCCGGCCCGCTCACCTGCTGATGGCGCTGCTGACCCAAAACGACGGTATCGCCGCACCGCTACTGGAGGCTGTCGGTGTCGAGCCCGCCACCGTCCGCGCCGAAACCCAGCGCCTGCTCGACCGTTTGCCGCAGGCGACTGGAGCCAGCACGCAGCCGCAGCTGTCCCGCGAGTCGTTAGCGGCGATCACCACCGCGCAGCAGCTGGCCACCGAGCTGGACGACGAGTACGTCTCCACCGAGCACGTGATGGTCGGGCTGGCCACCGGTGACTCCGACGTCGCCAAGCTGTTGACCGGCCACGGCGCCTCGCCGCAGGCGCTGCGGGAGGCGTTCGTCAAGGTGCGCGGCAGCGCCCGGGTCACCAGCCCCGAACCGGAGGCGACCTATCAGGCGCTGCAGAAGTACTCCACCGACCTGACCGCCCGCGCCCGCGAAGGCAAACTCGACCCGGTCATCGGCCGCGACAACGAGATCCGCCGCGTGGTGCAGGTGCTGTCCCGTCGCACCAAGAACAACCCGGTGCTGATCGGTGAGCCCGGCGTCGGCAAGACCGCGATCGTGGAGGGCCTGGCGCAGCGCATCGTGGCCGGCGACGTGCCGGAGAGCTTGCGCGACAAGACCATCGTCGCGCTCGATCTCGGCTCGATGGTCGCCGGCTCCAAATACCGCGGCGAATTCGAGGAACGGCTCAAGGCCGTCCTCGACGACATCAAGAACTCGGCCGGCCAAATCATCACGTTCATCGACGAGCTGCACACCATCGTCGGCGCCGGCGCCACCGGCGAGGGGGCGATGGACGCCGGCAACATGATCAAGCCGATGCTGGCCCGCGGCGAGTTACGGCTGGTCGGGGCGACCACGCTGGACGAATACCGCAAGCACATCGAGAAGGACGCCGCGCTCGAGCGCCGTTTCCAACAGGTGTACGTCGGCGAGCCGTCGGTGGAGGACACCATCGGCATCCTGCGCGGGCTCAAAGACCGCTACGAGGTGCACCACGGGGTGCGCATCACCGACTCGGCGCTGGTGGCAGCTGCCACTTTGAGCGACCGGTATATCACCGCCCGCTTCCTGCCCGACAAGGCCATCGACCTGGTCGACGAGGCGGCCAGCCGGCTGCGGATGGAGATCGACTCGCGGCCCGTCGAGATCGACGAGGTCGAGCGGCTGGTGCGCCGGCTGGAGATCGAAGAGATGGCGCTGTCCAAAGAAGAAGACGAGGCGTCGGCGGAGCGGTTGGCCAAGCTGCGCTCCGAGCTGGCCGACCAGAAAGAGAAGTTGGCCGAGCTCACCACCCGCTGGCAGAACGAGAAGAACGCGATCGAAATCGTCCGCGACCTCAAGGAGCAGCTGGAAGCCCTGCGCGGGGAATCCGAGCGGGCCGAACGCGACGGCGACCTGGCCAAGGCCGCCGAGCTGCGCTACGGACGCATCCCCGAGGTGGAGAAGAAGCTCGACGCGGCGTTGCCGCAGGCGCAGGCCCGGGAGCAGGTGATGCTCAAGGAGGAGGTCGGTCCCGACGACATCGCCGACGTGGTGTCGGCGTGGACCGGCATCCCGGCCGGTCGGCTGCTGGAAGGCGAGACCGCCAAGCTGCTGCGCATGGAAGACGAGCTGGGCAAGCGGGTCATCGGGCAGAAGGCCGCGGTTACCGCAGTCTCTGATGCGGTGCGGCGCAGCCGGGCCGGGGTGTCCGACCCCAACCGGCCCACCGGGGCGTTCATGTTCCTCGGCCCGACCGGTGTCGGCAAGACCGAGCTGGCCAAGGCGCTGGCCGACTTCCTGTTCGACGACGAGCGGGCGATGGTCCGCATCGACATGAGCGAGTACGGCGAGAAGCACACCGTGGCTCGGTTGATCGGCGCCCCGCCCGGCTATGTGGGATACGAGGCGGGCGGTCAGCTGACCGAGGCGGTGCGCCGGCGTCCCTACACGGTGGTGCTGTTCGACGAGATCGAGAAGGCGCACCCGGACGTGTTCGACGTGCTGCTGCAGGTCCTCGACGAGGGCCGGCTCACCGACGGGCACGGCCGCACGGTCGACTTCCGCAACACCATCTTGATCCTGACGTCCAACCTGGGGTCGGGTGGCAGCGCCGAGCAGGTGCTGGCCGCGGTGCGCGCTACGTTCAAGCCGGAGTTCATCAACCGGCTCGACGACGTGCTCATCTTTGAGGGTCTCAACCCCGAAGAGCTGGTGCGCATCGTCGACATCCAGCTGGCGCAGCTGGGCAAGCGGCTGGCGCAGCGGCGGCTGCAGCTGCAGGTCTCGCTGCCGGCCAAGCGCTGGTTGGCGCAGCGCGGATTCGACCCGGTGTACGGGGCGCGGCCGTTGCGCCGGCTGGTGCAGCAGGCCATCGGTGACCAGCTGGCCAAGATGCTGTTGGCCGGCCAGGTGCACGACGGCGATACCGTGCCGGTCAACGTCAGCCCCGACGCCGACTCGCTGATCCTGGGCTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity). {ECO:0000250}.

Family

ClpA/ClpB family

GO

protein metabolic process GO:0019538 biological_process
response to heat GO:0009408 biological_process
protein refolding GO:0042026 biological_process
cell wall GO:0005618 cellular_component
ATP binding GO:0005524 molecular_function
plasma membrane GO:0005886 cellular_component
cytoplasm GO:0005737 cellular_component

InterPro

UniProt: P9WPD1
GenBank: Rv0384c
EnsemblBacteria: Rv0384c
Mycobrowser: Rv0384c

Summary

Name: Chaperone protein ClpB
Family: ClpA/ClpB family
Protein Sequence: MDSFNPTTKTQAALTAALQAASTAGNPEIRPAHLLMALLTQNDGIAAPLLEAVGVEPATVRAETQRLLDRLPQATGASTQPQLSRESLAAITTAQQLATELDDEYVSTEHVMVGLATGDSDVAKLLTGHGASPQALREAFVKVRGSARVTSPEPEATYQALQKYSTDLTARAREGKLDPVIGRDNEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPESLRDKTIVALDLGSMVAGSKYRGEFEERLKAVLDDIKNSAGQIITFIDELHTIVGAGATGEGAMDAGNMIKPMLARGELRLVGATTLDEYRKHIEKDAALERRFQQVYVGEPSVEDTIGILRGLKDRYEVHHGVRITDSALVAAATLSDRYITARFLPDKAIDLVDEAASRLRMEIDSRPVEIDEVERLVRRLEIEEMALSKEEDEASAERLAKLRSELADQKEKLAELTTRWQNEKNAIEIVRDLKEQLEALRGESERAERDGDLAKAAELRYGRIPEVEKKLDAALPQAQAREQVMLKEEVGPDDIADVVSAWTGIPAGRLLEGETAKLLRMEDELGKRVIGQKAAVTAVSDAVRRSRAGVSDPNRPTGAFMFLGPTGVGKTELAKALADFLFDDERAMVRIDMSEYGEKHTVARLIGAPPGYVGYEAGGQLTEAVRRRPYTVVLFDEIEKAHPDVFDVLLQVLDEGRLTDGHGRTVDFRNTILILTSNLGSGGSAEQVLAAVRATFKPEFINRLDDVLIFEGLNPEELVRIVDIQLAQLGKRLAQRRLQLQVSLPAKRWLAQRGFDPVYGARPLRRLVQQAIGDQLAKMLLAGQVHDGDTVPVNVSPDADSLILG
Mass: 92,568 Da
Length: 848 Aa

Rv0384c doesn't seem to be a targeted by any drug.

Rv0384c doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
The alternative sigma factor SigH regulates major components of oxidative and heat stress responses in Mycobacterium tuberculosis.: J Bacteriol. 2001 Oct;183(20):6119-25. doi: 10.1128/JB.183.20.6119-6125.2001.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.