Gene - combatTB X

pknG

Type: protein_coding
Name: pknG
Locus Name: Rv0410c
Product: Serine/threonine-protein kinase PknG (protein kinase G) (STPK G)
Functional Category: Regulatory proteins
Location: 495062..497314 (- strand)
Gene Length: 2252 bp
Nucleotides: ATGGCCAAAGCGTCAGAGACCGAACGTTCGGGCCCCGGCACCCAACCGGCGGACGCCCAGACCGCGACGTCCGCGACGGTTCGACCCCTGAGCACCCAGGCGGTGTTCCGCCCCGATTTCGGCGATGAGGACAACTTCCCCCATCCGACGCTCGGCCCGGACACCGAGCCGCAAGACCGGATGGCCACCACCAGCCGGGTGCGCCCGCCGGTCAGACGGCTGGGCGGCGGCCTGGTGGAAATCCCGCGGGCGCCCGATATCGATCCGCTTGAGGCCCTGATGACCAACCCGGTGGTGCCGGAGTCCAAGCGGTTCTGCTGGAACTGTGGACGTCCCGTCGGCCGGTCCGACTCGGAGACCAAGGGAGCTTCAGAGGGCTGGTGTCCCTATTGCGGCAGCCCGTATTCGTTCCTGCCGCAGCTAAATCCCGGGGACATCGTCGCCGGCCAGTACGAGGTCAAAGGCTGCATCGCGCACGGCGGACTGGGCTGGATCTACCTCGCTCTCGACCGCAATGTCAACGGCCGTCCGGTGGTGCTCAAGGGCCTGGTGCATTCCGGTGATGCCGAAGCGCAGGCAATGGCGATGGCCGAACGCCAGTTCCTGGCCGAGGTGGTGCACCCGTCGATCGTGCAGATCTTCAACTTTGTCGAGCACACCGACAGGCACGGGGATCCGGTCGGCTACATCGTGATGGAATACGTCGGCGGGCAATCGCTCAAACGCAGCAAGGGTCAGAAACTGCCCGTCGCGGAGGCCATCGCCTACCTGCTGGAGATCCTGCCGGCGCTGAGCTACCTGCATTCCATCGGCTTGGTCTACAACGACCTGAAGCCGGAAAACATCATGCTGACCGAGGAACAGCTCAAGCTGATCGACCTGGGCGCGGTATCGCGGATCAACTCGTTCGGCTACCTCTACGGGACCCCAGGCTTCCAGGCGCCCGAGATCGTGCGGACCGGTCCGACGGTGGCCACCGACATCTACACCGTGGGACGCACGCTCGCGGCGCTCACGCTGGACCTGCCCACCCGCAATGGCCGTTATGTGGATGGGCTACCCGAAGACGACCCGGTGCTGAAAACCTACGACTCTTACGGCCGGTTGCTGCGCAGGGCCATCGACCCCGATCCGCGGCAACGGTTCACCACCGCCGAAGAGATGTCCGCGCAATTGACGGGCGTGTTGCGGGAGGTGGTCGCCCAGGACACCGGGGTGCCGCGGCCAGGGCTATCAACGATCTTCAGTCCCAGTCGGTCGACATTTGGAGTGGACCTGCTGGTGGCGCACACCGACGTGTATCTGGACGGGCAGGTGCACGCGGAGAAGCTGACCGCCAACGAGATCGTGACCGCGCTGTCGGTGCCGCTGGTCGATCCGACCGACGTCGCAGCTTCGGTCCTGCAGGCCACGGTGCTCTCCCAGCCGGTGCAGACCCTAGACTCGCTGCGCGCGGCCCGCCACGGTGCGCTGGACGCCGACGGCGTCGACTTCTCCGAGTCAGTGGAGCTGCCGCTAATGGAAGTCCGCGCGCTGCTGGATCTCGGCGATGTGGCCAAGGCCACCCGAAAACTCGACGATCTGGCCGAACGCGTTGGCTGGCGATGGCGATTGGTCTGGTACCGGGCCGTCGCCGAGCTGCTCACCGGCGACTATGACTCGGCCACCAAACATTTCACCGAGGTGCTGGATACCTTTCCCGGCGAGCTGGCGCCCAAGCTCGCCCTGGCCGCCACCGCCGAACTAGCCGGCAACACCGACGAACACAAGTTCTATCAGACGGTGTGGAGCACCAACGACGGCGTGATCTCGGCGGCTTTCGGACTGGCCAGAGCCCGGTCGGCCGAAGGTGATCGGGTCGGCGCCGTGCGCACGCTCGACGAGGTACCGCCCACTTCTCGGCATTTCACCACGGCACGGCTGACCAGCGCGGTGACTCTGTTGTCCGGCCGGTCAACGAGTGAAGTCACCGAGGAACAGATCCGCGACGCCGCCCGAAGAGTGGAGGCGCTGCCCCCGACCGAACCACGCGTGCTGCAGATCCGCGCCCTGGTGCTGGGTGGCGCGCTGGACTGGCTGAAGGACAACAAGGCCAGCACCAACCACATCCTCGGTTTCCCGTTCACCAGTCACGGGCTGCGGCTGGGTGTCGAGGCGTCACTGCGCAGCCTGGCCCGGGTAGCTCCCACTCAACGGCATCGCTACACGCTGGTGGACATGGCCAACAAGGTCCGGCCCACCAGCACGTTCTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Phosphorylates GarA. May play a role in metabolic regulation via control of the phosphorylation status of GarA. Plays a crucial role in the survival of mycobacteria within host macrophages, by blocking the intracellular degradation of mycobacteria in lysosomes. Required for intrinsic antibiotic resistance. {ECO:0000269|PubMed:17616581, ECO:0000269|PubMed:19019160, ECO:0000269|PubMed:19528288, ECO:0000269|PubMed:19638631}.

Family

Protein kinase superfamily, Ser/Thr protein kinase family

GO

avoidance of host defenses GO:0044413 biological_process
protein serine/threonine kinase activity GO:0004674 molecular_function
glutamine catabolic process GO:0006543 biological_process
glutamate catabolic process GO:0006538 biological_process
negative regulation of cytolysis by symbiont of host cells GO:0001899 biological_process
modulation by symbiont of host signal transduction pathway GO:0052027 biological_process
plasma membrane GO:0005886 cellular_component
identical protein binding GO:0042802 molecular_function
modulation by symbiont of host phagocytosis GO:0052190 biological_process
cytosol GO:0005829 cellular_component
ATP binding GO:0005524 molecular_function
positive regulation by symbiont of defense-related host reactive oxygen species production GO:0052369 biological_process
pathogenesis GO:0009405 biological_process
response to antibiotic GO:0046677 biological_process
protein autophosphorylation GO:0046777 biological_process

InterPro

UniProt: P9WI73
GenBank: Rv0410c
EnsemblBacteria: Rv0410c
Mycobrowser: Rv0410c

Summary

Name: Serine/threonine-protein kinase PknG (EC 2.7.11.1)
Family: Protein kinase superfamily, Ser/Thr protein kinase family
Protein Sequence: MAKASETERSGPGTQPADAQTATSATVRPLSTQAVFRPDFGDEDNFPHPTLGPDTEPQDRMATTSRVRPPVRRLGGGLVEIPRAPDIDPLEALMTNPVVPESKRFCWNCGRPVGRSDSETKGASEGWCPYCGSPYSFLPQLNPGDIVAGQYEVKGCIAHGGLGWIYLALDRNVNGRPVVLKGLVHSGDAEAQAMAMAERQFLAEVVHPSIVQIFNFVEHTDRHGDPVGYIVMEYVGGQSLKRSKGQKLPVAEAIAYLLEILPALSYLHSIGLVYNDLKPENIMLTEEQLKLIDLGAVSRINSFGYLYGTPGFQAPEIVRTGPTVATDIYTVGRTLAALTLDLPTRNGRYVDGLPEDDPVLKTYDSYGRLLRRAIDPDPRQRFTTAEEMSAQLTGVLREVVAQDTGVPRPGLSTIFSPSRSTFGVDLLVAHTDVYLDGQVHAEKLTANEIVTALSVPLVDPTDVAASVLQATVLSQPVQTLDSLRAARHGALDADGVDFSESVELPLMEVRALLDLGDVAKATRKLDDLAERVGWRWRLVWYRAVAELLTGDYDSATKHFTEVLDTFPGELAPKLALAATAELAGNTDEHKFYQTVWSTNDGVISAAFGLARARSAEGDRVGAVRTLDEVPPTSRHFTTARLTSAVTLLSGRSTSEVTEEQIRDAARRVEALPPTEPRVLQIRALVLGGALDWLKDNKASTNHILGFPFTSHGLRLGVEASLRSLARVAPTQRHRYTLVDMANKVRPTSTF
Mass: 81,577 Da
Length: 750 Aa

Rv0410c doesn't seem to be a targeted by any drug.

Tuberculosis mtu05152
mtu05152
Human Diseases; Infectious disease: bacterial

Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis.: Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12151-6. doi: 10.1073/pnas.0702842104. Epub 2007 Jul 6.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Key residues in Mycobacterium tuberculosis protein kinase G play a role in regulating kinase activity and survival in the host.: J Biol Chem. 2009 Oct 2;284(40):27467-79. doi: 10.1074/jbc.M109.036095. Epub 2009 Jul 28.
Protein kinase G is required for intrinsic antibiotic resistance in mycobacteria.: Antimicrob Agents Chemother. 2009 Aug;53(8):3515-9. doi: 10.1128/AAC.00012-09. Epub 2009 Jun 15.
Regulation of glutamate metabolism by protein kinases in mycobacteria.: Mol Microbiol. 2008 Dec;70(6):1408-23. doi: 10.1111/j.1365-2958.2008.06489.x. Epub 2008 Oct 17.
Serine/threonine protein kinases PknF and PknG of Mycobacterium tuberculosis: characterization and localization.: Microbiology. 2001 Aug;147(Pt 8):2307-14. doi: 10.1099/00221287-147-8-2307.
The Mycobacterium tuberculosis protein serine/threonine kinase PknG is linked to cellular glutamate/glutamine levels and is important for growth in vivo.: Mol Microbiol. 2004 Jun;52(6):1691-702. doi: 10.1111/j.1365-2958.2004.04085.x.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.