Gene - combatTB X

thiG

Type: protein_coding
Name: thiG
Locus Name: Rv0417
Product: Probable thiamin biosynthesis protein ThiG (thiazole biosynthesis protein)
Functional Category: Intermediary metabolism and respiration
Location: 502366..503124 (+ strand)
Gene Length: 758 bp
Nucleotides: TGGCTGAGTCCAAGTTGGTTATCGGTGACCGCAGCTTCGCCTCGCGGCTCATCATGGGTACTGGGGGTGCGACCAATCTGGCGGTGCTAGAGCAGGCTCTGATCGCCTCAGGTACCGAGCTGACCACCGTCGCGATACGCCGGGTCGACGCCGACGGGGGAACCGGCCTGCTCGACCTGCTCAACCGGCTCGGCATCACACCGCTACCCAACACCGCGGGGTCCCGCAGCGCCGCGGAAGCGGTCCTGACAGCGCAGTTGGCCCGTGAGGCGCTGAACACCAACTGGGTCAAGCTCGAGGTGATTGCCGACGAACGCACCCTGTGGCCTGATGCGGTCGAATTAGTCCGGGCTGCAGAACAATTGGTGGACGACGGATTTGTGGTCCTACCGTACACAACCGACGACCCGGTGCTGGCCCGCCGGCTAGAAGATACCGGTTGCGCAGCGGTGATGCCGCTGGGTTCGCCGATCGGCACCGGCCTTGGTATCGCCAACCCGCACAATATCGAGATGATCGTCGCCGGTGCCCGCGTTCCCGTGGTGCTGGACGCGGGCATCGGTACCGCCAGCGATGCCGCGTTGGCGATGGAGTTGGGTTGCGATGCCGTGTTGTTGGCCAGTGCGGTGACCCGGGCCGCCGACCCGCCGGCGATGGCCGCGGCGATGGCCGCCGCGGTGACCGCCGGATATCTGGCGCGTTGCGCGGGGCGGATCCCGAAACGCTTCTGGGCTCAGGCTTCCAGCCCGGCACGATAA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S. {ECO:0000255|HAMAP-Rule:MF_00443}.

Family

ThiG family

GO

thiamine biosynthetic process GO:0009228 biological_process
thiamine diphosphate biosynthetic process GO:0009229 biological_process
cytoplasm GO:0005737 cellular_component
sulfurtransferase activity GO:0016783 molecular_function

InterPro

UniProt: P9WG73
GenBank: Rv0417
EnsemblBacteria: Rv0417
Mycobrowser: Rv0417

Summary

Name: Thiazole synthase (EC 2.8.1.10)
Family: ThiG family
Protein Sequence: MAESKLVIGDRSFASRLIMGTGGATNLAVLEQALIASGTELTTVAIRRVDADGGTGLLDLLNRLGITPLPNTAGSRSAAEAVLTAQLAREALNTNWVKLEVIADERTLWPDAVELVRAAEQLVDDGFVVLPYTTDDPVLARRLEDTGCAAVMPLGSPIGTGLGIANPHNIEMIVAGARVPVVLDAGIGTASDAALAMELGCDAVLLASAVTRAADPPAMAAAMAAAVTAGYLARCAGRIPKRFWAQASSPAR
Mass: 25,878 Da
Length: 252 Aa

Rv0417 doesn't seem to be a targeted by any drug.

Thiamine metabolism mtu00730
mtu00730
PATHWAY_MAP mtu00730 Thiamine metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.