Gene - combatTB X

thiC

Type: protein_coding
Name: thiC
Locus Name: Rv0423c
Product: Probable thiamine biosynthesis protein ThiC
Functional Category: Intermediary metabolism and respiration
Location: 508582..510225 (- strand)
Gene Length: 1643 bp
Nucleotides: ATGACCATCACCGTTGAACCGTCGGTGACCACGGGTCCCATCGCGGGCAGCGCCAAGGCCTACCGTGAAATCGAGGCTCCCGGCAGCGGAGCTACTCTCCAAGTCCCGTTTCGACGGGTGCACTTGTCCACCGGAGACCACTTCGACCTCTACGACACCTCCGGGCCCTACACCGACACGGACACGGTGATCGACCTGACCGCGGGGCTGCCGCATAGGCCCGGAGTGGTTCGCGATCGGGGCACCCAGCTGCAGCGGGCCCGCGCCGGGGAGATCACCGCCGAGATGGCGTTCATCGCCGCCCGCGAAGACATGTCCGCCGAGCTAGTGCGCGACGAGGTCGCCCGCGGCCGCGCGGTGATCCCGGCCAACCACCACCACCCCGAGAGCGAGCCGATGATCATCGGCAAGGCGTTCGCGGTGAAAGTCAACGCCAACATCGGCAACTCGGCGGTGACGAGCTCGATCGCCGAGGAGGTCGACAAGATGGTGTGGGCCACCCGCTGGGGGGCCGACACCATCATGGACCTGTCCACCGGCAAGAACATCCACGAAACCCGCGAGTGGATCCTGCGCAATTCTCCCGTGCCGGTCGGCACCGTGCCGATCTATCAGGCGCTGGAGAAAGTCAAGGGCGATCCGACCGAGCTGACCTGGGAGATCTACCGCGACACCGTGATCGAGCAGTGTGAGCAAGGCGTGGACTACATGACGGTGCACGCCGGGGTGCTGCTGCGGTATGTGCCGCTGACCGCCAAGCGGGTCACCGGCATCGTGTCCCGCGGGGGTTCGATCATGGCCGCGTGGTGTTTGGCACATCATCGGGAGTCGTTCTTGTACACCAACTTTGAGGAGCTCTGCGATATTTTCGCCCGCTACGACGTCACCTTCTCACTCGGTGACGGGCTGCGACCAGGGTCGATCGCTGATGCCAACGACGCCGCGCAGTTCGCCGAGCTGCGCACCCTGGGCGAGCTCACCAAGATCGCCAAAGCCCATGGCGCACAGGTGATGATCGAGGGGCCGGGCCATATCCCAATGCACAAGATCGTCGAGAATGTGCGGCTGGAAGAGGAACTGTGTGAGGAGGCCCCGTTCTACACGCTGGGTCCGCTGGCCACCGACATCGCGCCGGCCTACGACCACATCACCTCGGCGATCGGTGCGGCCATCATCGCCCAAGCCGGTACCGCGATGCTGTGCTACGTCACCCCCAAGGAGCACCTCGGGTTGCCGGACCGCAAGGACGTCAAGGACGGGGTGATCGCCTACAAGATCGCCGCGCATGCGGCCGATTTGGCCAAGGGCCATCCGCGCGCCCAGGAGCGCGACGACGCTTTGAGCACGGCGCGTTTCGAGTTCCGCTGGAACGACCAGTTCGCACTGTCGCTGGATCCCGACACCGCACGGGAATTCCACGACGAAACCCTGCCGGCGGAGCCGGCCAAGACCGCGCACTTCTGCTCGATGTGCGGACCGAAGTTCTGCTCCATGCGCATCACCCAGGACGTCCGTGAGTACGCCGCCGAACACGGGCTTGAGACCGAAGCGGACATCGAAGCCGTGCTCGCCGCCGGAATGGCCGAAAAGTCACGTGAATTCGCAGAGCACGGCAATCGGGTGTATCTCCCGATAACCCAGTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction. {ECO:0000255|HAMAP-Rule:MF_00089}.

Family

ThiC family

GO

thiamine biosynthetic process GO:0009228 biological_process
thiamine diphosphate biosynthetic process GO:0009229 biological_process
cytosol GO:0005829 cellular_component
plasma membrane GO:0005886 cellular_component
4 iron, 4 sulfur cluster binding GO:0051539 molecular_function
zinc ion binding GO:0008270 molecular_function
carbon-carbon lyase activity GO:0016830 molecular_function

InterPro

UniProt: P9WG79
GenBank: Rv0423c
EnsemblBacteria: Rv0423c
Mycobrowser: Rv0423c

Summary

Name: Phosphomethylpyrimidine synthase (EC 4.1.99.17) (Hydroxymethylpyrimidine phosphate synthase) (HMP-P synthase) (HMP-phosphate synthase) (HMPP synthase) (Thiamine biosynthesis protein ThiC)
Family: ThiC family
Protein Sequence: MTITVEPSVTTGPIAGSAKAYREIEAPGSGATLQVPFRRVHLSTGDHFDLYDTSGPYTDTDTVIDLTAGLPHRPGVVRDRGTQLQRARAGEITAEMAFIAAREDMSAELVRDEVARGRAVIPANHHHPESEPMIIGKAFAVKVNANIGNSAVTSSIAEEVDKMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTELTWEIYRDTVIEQCEQGVDYMTVHAGVLLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFEELCDIFARYDVTFSLGDGLRPGSIADANDAAQFAELRTLGELTKIAKAHGAQVMIEGPGHIPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYVTPKEHLGLPDRKDVKDGVIAYKIAAHAADLAKGHPRAQERDDALSTARFEFRWNDQFALSLDPDTAREFHDETLPAEPAKTAHFCSMCGPKFCSMRITQDVREYAAEHGLETEADIEAVLAAGMAEKSREFAEHGNRVYLPITQ
Mass: 59,898 Da
Length: 547 Aa

Rv0423c doesn't seem to be a targeted by any drug.

Thiamine metabolism mtu00730
mtu00730
PATHWAY_MAP mtu00730 Thiamine metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.