Gene - combatTB X

menD

Type: protein_coding
Name: menD
Locus Name: Rv0555
Product: Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha-ketoglutarate decarboxylase) (KDC)
Functional Category: Intermediary metabolism and respiration
Location: 646298..647962 (+ strand)
Gene Length: 1664 bp
Nucleotides: TGAACCCCTCGACGACACAGGCGCGCGTCGTCGTCGACGAACTGATCCGCGGCGGCGTTCGCGACGTGGTGCTGTGTCCGGGCTCGCGCAATGCGCCGCTGGCCTTCGCGCTGCAGGACGCCGACCGGTCCGGCCGGATCCGGTTGCACGTTCGCATCGATGAACGCACCGCCGGCTACCTGGCCATCGGGCTGGCAATCGGGGCGGGCGCGCCGGTGTGTGTCGCGATGACATCCGGCACCGCCGTGGCCAACCTCGGTCCGGCGGTGGTGGAGGCAAACTACGCTCGGGTGCCGCTGATCGTGCTGTCAGCCAATCGGCCCTACGAGCTGCTGGGCACCGGCGCCAACCAGACCATGGAACAGCTGGGCTATTTCGGCACCCAGGTGCGCGCCAGCATCAGCCTGGGGCTGGCCGAGGACGCACCCGAGCGGACCTCGGCGCTCAACGCGACCTGGCGATCGGCTACGTGCCGAGTGTTGGCGGCCGCCACGGGTGCTCGCACCGCCAACGCGGGCCCCGTGCACTTCGACATCCCGCTGCGCGAACCGCTGGTGCCCGATCCCGAGCCCCTCGGCGCGGTCACCCCGCCGGGCCGGCCTGCTGGCAAGCCGTGGACCTACACGCCGCCGGTCACCTTCGACCAGCCACTGGACATCGACCTGTCGGTCGACACCGTGGTCATCTCCGGGCATGGCGCTGGCGTGCACCCCAACCTCGCGGCGTTGCCGACCGTCGCAGAACCGACGGCGCCGCGGTCCGGGGACAACCCGTTGCACCCGCTGGCGCTGCCGCTGCTGCGCCCTCAACAGGTGATCATGCTGGGCCGGCCGACACTGCATCGTCCGGTATCGGTGCTGCTGGCCGACGCAGAAGTGCCGGTATTCGCATTGACAACCGGTCCACGCTGGCCGGATGTCTCGGGTAACTCGCAGGCCACCGGCACGCGGGCGGTCACCACCGGCGCGCCGCGGCCCGCGTGGCTGGACCGGTGTGCGGCGATGAACCGGCACGCGATCGCGGCGGTTCGGGAACAGCTCGCGGCGCACCCGTTGACCACCGGGCTGCATGTCGCGGCGGCGGTGTCGCATGCGCTGCGGCCCGGTGACCAGCTGGTGCTCGGGGCATCCAATCCGGTGCGGGATGTGGCGTTGGCCGGTTTGGACACCCGCGGCATCCGGGTACGGTCCAACCGTGGGGTCGCCGGCATCGACGGCACCGTGTCCACCGCGATCGGGGCGGCCCTAGCTTATGAGGGGGCTCACGAGCGCACCGGCAGCCCGGACTCCCCGCCCCGCACCATCGCACTGATCGGCGACCTGACGTTCGTGCACGACAGCTCCGGGCTGTTGATCGGGCCGACCGAACCGATACCGCGGTCATTGACCATCGTGGTGTCTAATGACAACGGCGGCGGCATCTTCGAATTGCTCGAGCAGGGTGATCCCAGGTTCTCCGACGTGTCATCGCGAATCTTCGGCACCCCACACGACGTCGATGTGGGCGCATTGTGCCGCGCCTACCACGTGGAATCTCGCCAGATCGAGGTCGACGAACTCGGACCGACCCTCGATCAACCCGGTGCCGGCATGCGCGTGCTCGAGGTCAAGGCCGACCGGTCGTCGTTGCGACAATTGCACGCCGCCATCAAGGCGGCTCTGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC). {ECO:0000255|HAMAP-Rule:MF_01659}.

Family

TPP enzyme family, MenD subfamily

GO

magnesium ion binding GO:0000287 molecular_function
menaquinone biosynthetic process GO:0009234 biological_process
manganese ion binding GO:0030145 molecular_function
thiamine pyrophosphate binding GO:0030976 molecular_function
plasma membrane GO:0005886 cellular_component
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity GO:0070204 molecular_function

InterPro

UniProt: P9WK11
GenBank: Rv0555
EnsemblBacteria: Rv0555
Mycobrowser: Rv0555

PDB IDs:

Summary

Name: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (SEPHCHC synthase) (EC 2.2.1.9) (Menaquinone biosynthesis protein MenD)
Family: TPP enzyme family, MenD subfamily
Protein Sequence: MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRSGRIRLHVRIDERTAGYLAIGLAIGAGAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRASISLGLAEDAPERTSALNATWRSATCRVLAAATGARTANAGPVHFDIPLREPLVPDPEPLGAVTPPGRPAGKPWTYTPPVTFDQPLDIDLSVDTVVISGHGAGVHPNLAALPTVAEPTAPRSGDNPLHPLALPLLRPQQVIMLGRPTLHRPVSVLLADAEVPVFALTTGPRWPDVSGNSQATGTRAVTTGAPRPAWLDRCAAMNRHAIAAVREQLAAHPLTTGLHVAAAVSHALRPGDQLVLGASNPVRDVALAGLDTRGIRVRSNRGVAGIDGTVSTAIGAALAYEGAHERTGSPDSPPRTIALIGDLTFVHDSSGLLIGPTEPIPRSLTIVVSNDNGGGIFELLEQGDPRFSDVSSRIFGTPHDVDVGALCRAYHVESRQIEVDELGPTLDQPGAGMRVLEVKADRSSLRQLHAAIKAAL
Mass: 57,836 Da
Length: 554 Aa

Rv0555 doesn't seem to be a targeted by any drug.

Ubiquinone and other terpenoid-quinone biosynthesis mtu00130
mtu00130
Metabolism; Metabolism of cofactors and vitamins

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.