Gene - combatTB X

TB27.3

Type: protein_coding
Name: TB27.3
Locus Name: Rv0577
Product: Conserved protein TB27.3
Functional Category: Conserved hypotheticals
Location: 671166..671951 (+ strand)
Gene Length: 785 bp
Nucleotides: TGCCCAAGAGAAGCGAATACAGGCAAGGCACGCCGAACTGGGTCGACCTTCAGACCACCGATCAGTCCGCCGCCAAAAAGTTCTACACATCGTTGTTCGGCTGGGGTTACGACGACAACCCGGTCCCCGGAGGCGGTGGGGTCTATTCCATGGCCACGCTGAACGGCGAAGCCGTGGCCGCCATCGCACCGATGCCCCCGGGTGCACCGGAGGGGATGCCGCCGATCTGGAACACCTATATCGCGGTGGACGACGTCGATGCGGTGGTGGACAAGGTGGTGCCCGGGGGCGGGCAGGTGATGATGCCGGCCTTCGACATCGGCGATGCCGGCCGGATGTCGTTCATCACCGATCCGACCGGCGCTGCCGTGGGCCTATGGCAGGCCAATCGGCACATCGGAGCGACGTTGGTCAACGAGACGGGCACGCTCATCTGGAACGAACTGCTCACGGACAAGCCGGATTTGGCGCTAGCGTTCTACGAGGCTGTGGTTGGCCTCACCCACTCGAGCATGGAGATAGCTGCGGGCCAGAACTATCGGGTGCTCAAGGCCGGCGACGCGGAAGTCGGCGGCTGTATGGAACCGCCGATGCCCGGCGTGCCGAATCATTGGCACGTCTACTTTGCGGTGGATGACGCCGACGCCACGGCGGCCAAAGCCGCCGCAGCGGGCGGCCAGGTCATTGCGGAACCGGCTGACATTCCGTCGGTGGGCCGGTTCGCCGTGTTGTCCGATCCGCAGGGCGCGATCTTCAGTGTGTTGAAGCCCGCACCGCAGCAATAG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

May function as a glyoxylase involved in the methylglyoxal detoxification pathway (PubMed:20975714). Induces maturation of dendritic cells in a TLR2-dependent manner, causing increased expression of cell-surface molecules (CD80, CD86, MHC class I and II) and proinflammatory cytokines (TNF-alpha, IL-6, IL-1 beta and IL-12p70). Acts via both the NF-kappa-B and MAPK signaling pathways. Induces Th1-polarized immune responses (PubMed:22415304). {ECO:0000269|PubMed:20975714, ECO:0000269|PubMed:22415304}.

Family

Unknown

GO

pathogenesis GO:0009405 biological_process
extracellular region GO:0005576 cellular_component
plasma membrane GO:0005886 cellular_component

InterPro

UniProt: P9WIR3
GenBank: Rv0577
EnsemblBacteria: Rv0577
Mycobrowser: Rv0577

Summary

Name: Putative glyoxylase CFP32 (27 kDa antigen Cfp30B)
Family: Unknown
Protein Sequence: MPKRSEYRQGTPNWVDLQTTDQSAAKKFYTSLFGWGYDDNPVPGGGGVYSMATLNGEAVAAIAPMPPGAPEGMPPIWNTYIAVDDVDAVVDKVVPGGGQVMMPAFDIGDAGRMSFITDPTGAAVGLWQANRHIGATLVNETGTLIWNELLTDKPDLALAFYEAVVGLTHSSMEIAAGQNYRVLKAGDAEVGGCMEPPMPGVPNHWHVYFAVDDADATAAKAAAAGGQVIAEPADIPSVGRFAVLSDPQGAIFSVLKPAPQQ
Mass: 27,343 Da
Length: 261 Aa

Rv0577 doesn't seem to be a targeted by any drug.

Rv0577 doesn't seem to be involved in any pathway.

Mycobacterium tuberculosis Rv0577, a novel TLR2 agonist, induces maturation of dendritic cells and drives Th1 immune response.: FASEB J. 2012 Jun;26(6):2695-711. doi: 10.1096/fj.11-199588. Epub 2012 Mar 13.
Chemical shift assignments for Rv0577, a putative glyoxylase associated with virulence from Mycobacterium tuberculosis.: Biomol NMR Assign. 2012 Apr;6(1):43-6. doi: 10.1007/s12104-011-9322-5. Epub 2011 Jul 15.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
A chemical genetic screen in Mycobacterium tuberculosis identifies carbon-source-dependent growth inhibitors devoid of in vivo efficacy.: Nat Commun. 2010 Aug 24;1:57. doi: 10.1038/ncomms1060.
The Mycobacterium tuberculosis complex-restricted gene cfp32 encodes an expressed protein that is detectable in tuberculosis patients and is positively correlated with pulmonary interleukin-10.: Infect Immun. 2003 Dec;71(12):6871-83. doi: 10.1128/iai.71.12.6871-6883.2003.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.