Rv0669c
- Type
- protein_coding
- Name
- Rv0669c
- Locus Name
-
Rv0669c
- Product
-
Possible hydrolase
- Functional Category
-
Intermediary metabolism and respiration
- Location
- 767684..769597 (- strand)
- Gene Length
- 1913 bp
- Nucleotides
-
ATGCTTAGTGTAGGGCGCGGCATCGCCGACATCACTGGTGAGGCGGCGGACTGCGGCATGCTCGGCTACGGCAAGTCCGACCAGCGCACCGCCGGCATTCACCAGCGACTTAGGTCACGGGCGTTTGTGTTCCGGGACGATTCCCAGGACGGGGACGCGCGGTTGCTGCTGATTGTCGCCGAACTGCCGCTGCCCATGCAAAACGTGAATGAGGAAGTGCTGCGCCGGTTGGCCGATTTGTATGGCGATACCTACTCGGAGCAGAACACACTGATCACGGCGACCCACACCCACGCCGGGCCTGGCGGGTACTGCGGATACCTGCTGTACAACCTGACCACGAGCGGCTTTCGGCCGGCAACCTTTGCGGCGATCGTCGACGGAATTGTCGAATCGGTGGAACATGCGCATGCCGATGTGGCGCCCGCCGAGGTTTCGCTCTCGCACGGCGAGCTGTACGGCGCAAGCATCAATCGATCCCCATCAGCGTTCGACCGCAACCCGCCGGCCGACAAGGCGTTCTTCCCGAAACGCGTCGACCCGCATACCACGCTCGTGCGTATCGACCGGGGCGAGGCCACCGTGGGCGTGATCCATTTCTTCGCTACCCACGGTACGAGCATGACGAATCGCAACCACCTCATCTCCGGAGACAACAAGGGTTTCGCGGCCTATCACTGGGAACGCACCGTGGGCGGCGCGGATTATCTCGCCGGCCAGCCCGACTTCATCGCCGCCTTCGCACAGACGAATCCGGGCGACATGAGCCCGAATGTCGACGGGCCCCTTTCTCCCGAGGCCCCACCAGACCGGGAATTCGACAACACCCGTCGTACCGGGTTGTGCCAGTTCGAGGATGCTTTCACGCAGTTGAGCGGAGCGACGCCCATCGGCGCTGGCATTGATGCCCGGTTCACCTATGTCGATCTCGGTTCTGTGCTCGTGCGCGGCGAGTACACACCAGACGGTGAAGAGCGCCGCACCGGGCGTCCGATGTTTGGCGCCGGGGCCATGGCAGGCACCGACGAGGGCCCGGGCTTCCATGGCTTTCGCCAAGGGCGAAACCCGTTCTGGGACAGACTATCCCGCGCCATGTACCGGCTGGCAAGGCCGACGGCAGCGGCACAGGCCCCCAAAGGCATTGTGATGCCCGCACGCCTGCCGAACCGGATACACCCCTTTGTCCAGGAAATTGTTCCCGTTCAGCTCGTGCGAATCGGTCGCCTCTATCTGATCGGTATTCCGGGCGAACCGACCATCGTCGCGGGGCTTAGGCTGCGCCGCATGGTGGCTTCGATTGTTGGCGCCGACCTGGCCGACGTGCTCTGCGTGGGCTACACCAACGCCTACATCCATTACGTGACAACGCCCGAAGAGTATCTCGAACAGCGCTACGAGGGCGGCAGCACGTTGTTCGGTCGATGGGAACTGTGCGCCCTCATGCAGACCGTGGCCGAACTCGCCGAGGCCATGCGCGACGGCCGTCCGGTGACGCTCGGTCGCCGGCCGCGGCCCACCCGGGAGCTGAGCTGGGTGCGCGGCGCGCCCGCCGATGCCGGGTCGTTCGGAGCGGTCATCGCCGAGCCCTCGGCGACGTACCGACCGGGTCAGGCGGTGGAAGCCGTTTTCGTCAGCGCGCTGCCCAATAACGATCTGCGCCGCGGCGGCACCTACCTCGAAGTGGTCCGCCGGGAAGGTGCCAGCTGGGTGCGGATTGCTGACGACGGCGACTGGGCCACGAGCTTTCGCTGGCAGCGCCAAGGGCGGGCCGGATCGCATGTCAGCATTCGCTGGGATGTTCCCGGCGATACCACGCCCGGGCAATACCGCATCGTCCACCACGGCACCGCACGCGATCGCAACGGCATGCTTACAGCGTTCAGCGCGACGACGCGCGAGTTCACGGTTGTTTG
- Drug Resistance
- Mutations
- Function
- Catalyzes the cleavage of the N-acyl linkage of the ceramides (Cers) to yield sphingosine (Sph) and free fatty acid. Also catalyzes the synthesis of Cers from Sph and fatty acid. Cers containning C6-C24 fatty acids are well hydrolyzed, and Cers with mono unsaturated fatty acids are much more hydrolyzed than those with saturated fatty acids. {ECO:0000269|PubMed:10593963, ECO:0000269|PubMed:20139604}.
- Family
-
Neutral ceramidase family
- GO
-
- metal ion binding GO:0046872 molecular_function
- ceramide catabolic process GO:0046514 biological_process
- sphingosine biosynthetic process GO:0046512 biological_process
- N-acylsphingosine amidohydrolase activity GO:0017040 molecular_function
- cell wall GO:0005618 cellular_component
- extracellular region GO:0005576 cellular_component
- ceramidase activity GO:0102121 molecular_function
- long-chain fatty acid biosynthetic process GO:0042759 biological_process
- InterPro
Summary
- Name
- Neutral ceramidase (N-CDase) (NCDase) (EC 3.5.1.23) (Acylsphingosine deacylase) (N-acylsphingosine amidohydrolase)
- Family
- Neutral ceramidase family
- Protein Sequence
-
MLSVGRGIADITGEAADCGMLGYGKSDQRTAGIHQRLRSRAFVFRDDSQDGDARLLLIVAELPLPMQNVNEEVLRRLADLYGDTYSEQNTLITATHTHAGPGGYCGYLLYNLTTSGFRPATFAAIVDGIVESVEHAHADVAPAEVSLSHGELYGASINRSPSAFDRNPPADKAFFPKRVDPHTTLVRIDRGEATVGVIHFFATHGTSMTNRNHLISGDNKGFAAYHWERTVGGADYLAGQPDFIAAFAQTNPGDMSPNVDGPLSPEAPPDREFDNTRRTGLCQFEDAFTQLSGATPIGAGIDARFTYVDLGSVLVRGEYTPDGEERRTGRPMFGAGAMAGTDEGPGFHGFRQGRNPFWDRLSRAMYRLARPTAAAQAPKGIVMPARLPNRIHPFVQEIVPVQLVRIGRLYLIGIPGEPTIVAGLRLRRMVASIVGADLADVLCVGYTNAYIHYVTTPEEYLEQRYEGGSTLFGRWELCALMQTVAELAEAMRDGRPVTLGRRPRPTRELSWVRGAPADAGSFGAVIAEPSATYRPGQAVEAVFVSALPNNDLRRGGTYLEVVRREGASWVRIADDGDWATSFRWQRQGRAGSHVSIRWDVPGDTTPGQYRIVHHGTARDRNGMLTAFSATTREFTVV
- Mass
- 69,490 Da
- Length
- 637 Aa
Rv0669c doesn't seem to be a targeted by any drug.
-
-
Sphingolipid metabolism mtu00600
mtu00600PATHWAY_MAP mtu00600 Sphingolipid metabolism
-
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- Expression, purification, and characterization of a recombinant neutral ceramidase from Mycobacterium tuberculosis.
- Biosci Biotechnol Biochem. 2010;74(2):316-21. doi: 10.1271/bbb.90645. Epub 2010 Feb 7.
- Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. Cloning of a ceramidase homologue from Mycobacterium tuberculosis.
- J Biol Chem. 1999 Dec 17;274(51):36616-22. doi: 10.1074/jbc.274.51.36616.
- Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv.
- Microbiology. 2002 Oct;148(Pt 10):2967-73. doi: 10.1099/00221287-148-10-2967.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.