Gene - combatTB X

purL

Type: protein_coding
Name: purL
Locus Name: Rv0803
Product: Phosphoribosylformylglycinamidine synthase II PurL (FGAM synthase II)
Functional Category: Intermediary metabolism and respiration
Location: 895820..898084 (+ strand)
Gene Length: 2264 bp
Nucleotides: TGCTGGACACCGTCGAACACGCCGCGACCACACCCGACCAACCACAACCGTATGGTGAGCTGGGCCTCAAAGACGACGAGTACCGGCGGATTCGCCAGATCCTGGGCCGCCGGCCCACCGACACCGAGCTGGCCATGTACTCGGTGATGTGGAGCGAACACTGTTCGTACAAGTCCTCCAAGGTGCACCTGCGCTACTTCGGTGAGACCACCTCCGACGAGATGCGCGCGGCCATGCTGGCCGGCATCGGCGAGAACGCCGGCGTCGTCGACATCGGCGACGGCTGGGCGGTCACCTTCAAGGTGGAGTCACACAACCACCCGTCCTACGTCGAGCCCTACCAGGGCGCGGCCACCGGGGTGGGCGGCATCGTCCGCGACATCATGGCCATGGGCGCCCGACCGGTCGCCGTGATGGACCAGCTTCGGTTCGGCGCCGCCGACGCCCCCGATACCCGCCGCGTGCTCGACGGCGTGGTCCGCGGCATCGGCGGATACGGCAACTCCCTGGGCCTGCCCAACATTGGCGGAGAGACCGTCTTCGACCCGTGCTACGCCGGCAACCCCTTAGTGAACGCGTTGTGTGTCGGCGTATTACGGCAGGAGGACCTGCATTTGGCGTTCGCCTCCGGCGCCGGCAACAAGATCATCCTGTTTGGCGCGCGCACCGGGCTCGACGGTATCGGCGGGGTGTCGGTGCTGGCGTCGGACACCTTCGATGCCGAGGGATCCCGCAAGAAGCTGCCCTCGGTGCAGGTCGGCGACCCGTTCATGGAGAAGGTGCTCATCGAATGCTGTCTCGAGCTCTACGCGGGCGGCCTGGTGATCGGCATCCAAGACCTGGGCGGAGCCGGATTATCTTGTGCCACATCGGAGTTAGCATCCGCCGGTGATGGCGGAATGACGATCCAGCTGGACAGCGTCCCGCTGCGGGCCAAGGAGATGACGCCCGCCGAGGTGCTCTGCAGCGAATCGCAGGAGCGGATGTGCGCGGTGGTCTCCCCGAAGAACGTCGACGCATTCCTGGCGGTGTGCCGCAAGTGGGAGGTGCTGGCGACGGTGATCGGCGAGGTCACCGACGGCGACCGGCTGCAGATCACCTGGCACGGCGAGACGGTGGTCGACGTGCCGCCGCGCACCGTAGCTCACGAAGGTCCGGTATATCAGCGCCCGGTCGCCCGCCCCGATACGCAGGACGCGCTGAACGCCGACCGCTCGGCCAAGCTGTCACGGCCGGTCACCGGCGACGAGCTGCGCGCGACTTTGCTTGCGTTACTTGGCAGCCCGCACCTGTGCAGCCGCGCGTTCATCACCGAGCAGTACGACCGCTATGTGCGCGGCAACACGGTGCTCGCCGAGCACGCCGACGGCGGCATGCTGCGCATCGACGAGTCGACCGGCCGGGGCATCGCGGTATCGACCGACGCGTCGGGACGCTACACGCTGCTGGATCCCTACGCTGGCGCGCAACTCGCGTTGGCCGAGGCGTACCGCAACGTCGCCGTCACCGGCGCCACCCCGGTCGCGGTGACCAACTGCCTGAACTTCGGTTCCCCCGAGGACCCCGGCGTGATGTGGCAGTTCACGCAGGCGGTCCGCGGTCTGGCCGATGGCTGTGCGGACCTCGGGATTCCGGTGACCGGTGGCAACGTGAGTTTCTACAACCAAACCGGTTCGGCGGCAATCCTGCCCACGCCGGTGGTCGGGGTGCTCGGCGTCATCGACGATGTGCGTCGGCGCATCCCTACCGGCCTGGGCGCCGAGCCCGGGGAAACGTTGATGCTGTTGGGCGACACCCGCGACGAGTTCGACGGTTCCGTGTGGGCGCAGGTGACCGCAGACCACCTGGGTGGATTGCCGCCGGTAGTCGATCTGGCGCGGGAGAAGCTGCTGGCCGCGGTGCTGAGCTCGGCGTCGCGGGACGGGCTAGTGTCCGCGGCGCACGATCTGTCCGAGGGTGGGCTGGCCCAAGCCATCGTGGAATCGGCGTTAGCGGGTGAAACCGGTTGCCGCATAGTGCTTCCCGAAGGGGCTGACCCGTTTGTGCTGCTGTTCTCCGAGTCGGCGGGTCGGGTGCTGGTCGCGGTGCCACGCACCGAGGAGAGCCGGTTTCGCGGGATGTGTGAGGCGCGGGGACTTCCCGCGGTCCGCATCGGCGTCGTCGATCAAGGTTCGGACGCGGTTGAGGTGCAGGGCTTGTTCGCGGTGTCGTTGGCCGAACTGCGTGCGACATCCGAGGCGGTGTTGCCGCGATACTTCGGATGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL. {ECO:0000255|HAMAP-Rule:MF_00420, ECO:0000269|PubMed:8828210}.

Family

FGAMS family

GO

magnesium ion binding GO:0000287 molecular_function
ATP binding GO:0005524 molecular_function
cytoplasm GO:0005737 cellular_component
'de novo' IMP biosynthetic process GO:0006189 biological_process
purine nucleotide biosynthetic process GO:0006164 biological_process
phosphoribosylformylglycinamidine synthase activity GO:0004642 molecular_function

InterPro

UniProt: P9WHL7
GenBank: Rv0803
EnsemblBacteria: Rv0803
Mycobrowser: Rv0803

Summary

Name: Phosphoribosylformylglycinamidine synthase subunit PurL (FGAM synthase) (EC 6.3.5.3) (Formylglycinamide ribonucleotide amidotransferase subunit II) (FGAR amidotransferase II) (FGAR-AT II) (Glutamine amidotransferase PurL) (Phosphoribosylformylglycinamidine synthase subunit II)
Family: FGAMS family
Protein Sequence: MSPLARTPRKTSVLDTVEHAATTPDQPQPYGELGLKDDEYRRIRQILGRRPTDTELAMYSVMWSEHCSYKSSKVHLRYFGETTSDEMRAAMLAGIGENAGVVDIGDGWAVTFKVESHNHPSYVEPYQGAATGVGGIVRDIMAMGARPVAVMDQLRFGAADAPDTRRVLDGVVRGIGGYGNSLGLPNIGGETVFDPCYAGNPLVNALCVGVLRQEDLHLAFASGAGNKIILFGARTGLDGIGGVSVLASDTFDAEGSRKKLPSVQVGDPFMEKVLIECCLELYAGGLVIGIQDLGGAGLSCATSELASAGDGGMTIQLDSVPLRAKEMTPAEVLCSESQERMCAVVSPKNVDAFLAVCRKWEVLATVIGEVTDGDRLQITWHGETVVDVPPRTVAHEGPVYQRPVARPDTQDALNADRSAKLSRPVTGDELRATLLALLGSPHLCSRAFITEQYDRYVRGNTVLAEHADGGMLRIDESTGRGIAVSTDASGRYTLLDPYAGAQLALAEAYRNVAVTGATPVAVTNCLNFGSPEDPGVMWQFTQAVRGLADGCADLGIPVTGGNVSFYNQTGSAAILPTPVVGVLGVIDDVRRRIPTGLGAEPGETLMLLGDTRDEFDGSVWAQVTADHLGGLPPVVDLAREKLLAAVLSSASRDGLVSAAHDLSEGGLAQAIVESALAGETGCRIVLPEGADPFVLLFSESAGRVLVAVPRTEESRFRGMCEARGLPAVRIGVVDQGSDAVEVQGLFAVSLAELRATSEAVLPRYFG
Mass: 80,802 Da
Length: 766 Aa

Rv0803 doesn't seem to be a targeted by any drug.

Purine metabolism mtu00230
mtu00230
PATHWAY_MAP mtu00230 Purine metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
The Mycobacterium tuberculosis purine biosynthetic pathway: isolation and characterization of the purC and purL genes.: Microbiology. 1996 Sep;142 ( Pt 9):2439-47. doi: 10.1099/00221287-142-9-2439.