Gene - combatTB X

pstS3

Type: protein_coding
Name: pstS3
Locus Name: Rv0928
Product: Periplasmic phosphate-binding lipoprotein PstS3 (PBP-3) (PstS3) (PHOS1)
Functional Category: Cell wall and cell processes
Location: 1034903..1036015 (+ strand)
Gene Length: 1112 bp
Nucleotides: TGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAACGACGACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCGGCGAAGGTCGATTGCGGGGGGAAGAAGACACTCAAAGCCAGTGGGTCGACGGCGCAGGCCAACGCGATGACCCGCTTTGTCAACGTGTTCGAGCAGGCCTGCCCCGGCCAAACCCTGAACTACACGGCCAATGGTTCGGGCGCTGGAATCAGCGAATTTAATGGCAACCAAACCGATTTCGGTGGCTCAGATGTACCCCTGAGCAAGGACGAGGCCGCAGCGGCGCAGCGGCGTTGCGGCTCGCCGGCGTGGAATCTGCCGGTGGTGTTCGGCCCGATCGCGGTTACCTACAACCTCAACAGCGTTTCCTCGCTAAATTTGGACGGCCCCACGTTGGCGAAGATCTTCAACGGCTCCATTACGCAGTGGAACAATCCCGCGATCCAGGCGCTGAACCGCGACTTCACGCTGCCAGGTGAGCGGATTCACGTGGTGTTCCGCAGCGATGAGTCGGGGACCACGGACAACTTCCAGAGGTACCTGCAGGCCGCGTCCAACGGTGCGTGGGGTAAGGGCGCTGGAAAGTCGTTCCAAGGCGGCGTCGGTGAGGGCGCGCGGGGTAACGATGGCACGTCAGCGGCCGCGAAGAACACCCCGGGGTCGATCACCTACAACGAGTGGTCGTTCGCCCAGGCGCAGCACCTGACCATGGCCAACATCGTCACTTCGGCTGGTGGGGACCCGGTGGCGATTACTATCGACTCGGTCGGCCAGACGATCGCCGGGGCCACCATCTCCGGGGTGGGCAACGACCTGGTGCTCGACACGGACTCGTTCTACCGGCCGAAGCGTCCCGGCTCCTATCCGATCGTGTTAGCGACATACGAAATCGTTTGCTCGAAGTATCCCGACTCGCAGGTTGGCACGGCTGTGAAGGCGTTCCTGCAGAGCACTATCGGCGCCGGTCAAAGCGGCCTGGGGGACAACGGATACATCCCAATTCCGGACGAGTTCAAATCGAGGCTGTCGACTGCGGTCAACGCGATCGCCTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Functions in inorganic phosphate uptake, is probably the main carrier for phosphate uptake, it is the most highly expressed of the 3 PstS proteins under phosphate starvation (PubMed:20933472). Binds phosphate; probably able to bind both H(2)PO(4)(-) and HPO(4)(2-) (PubMed:24615888). Part of the ABC transporter complex PstSACB involved in phosphate import (Probable). Probably plays a role in host phagosome maturation arrest (PubMed:20844580). {ECO:0000269|PubMed:20933472, ECO:0000269|PubMed:24615888, ECO:0000305, ECO:0000305|PubMed:20844580}.

Family

PstS family

GO

growth of symbiont in host GO:0044117 biological_process
phosphate ion binding GO:0042301 molecular_function
cell wall GO:0005618 cellular_component
extracellular region GO:0005576 cellular_component
negative regulation by symbiont of host innate immune response GO:0052170 biological_process
phosphate ion transmembrane transport GO:0035435 biological_process
ATP-binding cassette (ABC) transporter complex GO:0043190 cellular_component
plasma membrane GO:0005886 cellular_component
cellular response to phosphate starvation GO:0016036 biological_process

InterPro

UniProt: P9WGT7
GenBank: Rv0928
EnsemblBacteria: Rv0928
Mycobrowser: Rv0928

Summary

Name: Phosphate-binding protein PstS 3 (PBP 3) (PstS-3) (Antigen Ag88)
Family: PstS family
Protein Sequence: MKLNRFGAAVGVLAAGALVLSACGNDDNVTGGGATTGQASAKVDCGGKKTLKASGSTAQANAMTRFVNVFEQACPGQTLNYTANGSGAGISEFNGNQTDFGGSDVPLSKDEAAAAQRRCGSPAWNLPVVFGPIAVTYNLNSVSSLNLDGPTLAKIFNGSITQWNNPAIQALNRDFTLPGERIHVVFRSDESGTTDNFQRYLQAASNGAWGKGAGKSFQGGVGEGARGNDGTSAAAKNTPGSITYNEWSFAQAQHLTMANIVTSAGGDPVAITIDSVGQTIAGATISGVGNDLVLDTDSFYRPKRPGSYPIVLATYEIVCSKYPDSQVGTAVKAFLQSTIGAGQSGLGDNGYIPIPDEFKSRLSTAVNAIA
Mass: 37,953 Da
Length: 370 Aa

Rv0928 doesn't seem to be a targeted by any drug.

Tuberculosis mtu05152
mtu05152
Human Diseases; Infectious disease: bacterial

Two-component system mtu02020
mtu02020
Environmental Information Processing; Signal transduction

ABC transporters mtu02010
mtu02010
Environmental Information Processing; Membrane transport

Crystal structure of the Mycobacterium tuberculosis phosphate binding protein PstS3.: Proteins. 2014 Sep;82(9):2268-74. doi: 10.1002/prot.24548. Epub 2014 Mar 24.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Effect of PstS sub-units or PknD deficiency on the survival of Mycobacterium tuberculosis.: Tuberculosis (Edinb). 2010 Nov;90(6):338-45. doi: 10.1016/j.tube.2010.09.004. Epub 2010 Oct 8.
High content phenotypic cell-based visual screen identifies Mycobacterium tuberculosis acyltrehalose-containing glycolipids involved in phagosome remodeling.: PLoS Pathog. 2010 Sep 9;6(9):e1001100. doi: 10.1371/journal.ppat.1001100.
Three different putative phosphate transport receptors are encoded by the Mycobacterium tuberculosis genome and are present at the surface of Mycobacterium bovis BCG.: J Bacteriol. 1997 May;179(9):2900-6. doi: 10.1128/jb.179.9.2900-2906.1997.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Identification of a second Mycobacterium tuberculosis gene cluster encoding proteins of an ABC phosphate transporter.: FEBS Lett. 1996 Sep 30;394(2):206-12.