Gene - combatTB X

pknD

Type: protein_coding
Name: pknD
Locus Name: Rv0931c
Product: Transmembrane serine/threonine-protein kinase D PknD (protein kinase D) (STPK D)
Functional Category: Regulatory proteins
Location: 1037920..1039914 (- strand)
Gene Length: 1994 bp
Nucleotides: GTGAGCGATGCCGTTCCGCAGGTAGGGTCGCAATTTGGGCCGTATCAGCTATTGCGGCTGCTGGGCCGAGGCGGGATGGGCGAGGTTTATGAGGCCGAGGACACCCGCAAGCACCGGGTGGTGGCGCTGAAGTTGATCTCGCCGCAGTACTCCGACAATGCGGTGTTTCGAGCACGGATGCAACGCGAGGCCGACACCGCGGGACGGCTGACCGAGCCACACATCGTGCCGATCCATGACTACGGCGAGATCAACGGACAGTTCTTTGTCGAAATGCGCATGATCGACGGCACCTCTTTACGCGCCCTATTGAAACAGTATGGTCCGCTGACCCCGGCCCGAGCTGTGGCCATCGTGCGCCAGATCGCCGCCGCTTTGGATGCCGCGCATGCCAACGGCGTAACGCACCGCGACGTAAAACCGGAAAACATTCTGGTCACCGCGAGCGACTTCGCCTATCTGGTCGATTTCGGTATTGCCCGTGCCGCCTCCGACCCGGGGCTGACCCAGACCGGGACCGCCGTGGGAACCTACAACTACATGGCCCCGGAGCGGTTCACCGGCGATGAGGTCACCTATCGCGCAGATATCTATGCGTTGGCGTGCGTGTTGGGTGAGTGTTTGACCGGTGCCCCACCGTATCGGGCCGACAGCGTTGAGCGGTTGATCGCCGCGCATCTAATGGACCCTGCCCCGCAGCCCAGCCAGCTACGGCCCGGGCGCGTGCCGCCGGCCCTGGATCAGGTGATCGCCAAAGGCATGGCCAAAAACCCCGCGGAGCGCTTTATGAGCGCTGGTGATCTGGCCATCGCCGCCCATGACGCACTCACCACATCCGAGCAACACCAGGCCACGACGATTCTGCGGCGCGGTGACAACGCAACCTTGCTGGCCACTCCGGCCGATACGGGCTTGAGCCAGTCCGAGTCCGGCATTGCCGGGGCGGGCACCGGCCCACCGACCCCGGGTGCTGCCCGATGGTCGCCCGGGGACTCCGCGACCGTGGCCGGACCCCTGGCAGCGGACAGCCGCGGCGGGAACTGGCCCAGCCAAACCGGACACTCCCCTGCCGTCCCGAATGCGCTCCAGGCCTCCCTGGGACACGCTGTGCCGCCGGCCGGCAACAAACGGAAGGTGTGGGCCGTCGTCGGCGCTGCCGCGATCGTACTGGTGGCCATCGTCGCCGCCGCCGGCTACCTGGTCTTACGACCTTCGTGGTCGCCGACGCAGGCATCCGGACAGACCGTGTTGCCGTTCACCGGCATCGACTTCCGCCTCTCGCCGAGTGGGGTGGCAGTGGACAGCGCCGGCAACGTGTACGTCACCAGTGAGGGCATGTACGGCCGAGTGGTCAAGTTGGCCACCGGGTCAACCGGCACGACGGTGTTACCGTTCAACGGGCTGTACCAGCCGCAGGGGTTGGCGGTGGACGGCGCTGGCACCGTATACGTCACCGATTTCAACAACCGGGTGGTGACGTTAGCGGCCGGGTCCAACAACCAGACCGTGCTACCGTTCGACGGCCTCAACTACCCCGAAGGTCTCGCGGTGGATACCCAGGGTGCGGTATACGTCGCCGACCGGGGCAACAACAGGGTGGTGAAGTTGGCGGCCGGATCCAAGACCCAGACCGTGCTGCCGTTCACCGGCCTCAACGACCCCGACGGGGTGGCCGTCGACAACTCCGGCAACGTCTATGTCACCGACACCGACAACAACAGGGTTGTCAAGCTAGAGGCCGAGTCGAATAACCAGGTCGTGCTGCCATTCACGGATATCACCGCACCGTGGGGCATCGCGGTGGACGAAGCCGGAACCGTCTACGTCACCGAGCACAACACAAACCAGGTGGTCAAACTCCTGGCCGGGTCGACCACCTCGACAGTGCTACCGTTCACCGGCCTCAACACCCCCCTCGCGGTGGCAGTGGACAGCGACCGGACCGTCTACGTCGCCGACCGCGGCAACGACCGAGTGGTGAAACTCACGTCATA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Key microbial factor required for central nervous system tuberculosis. Required for invasion of host brain endothelia, but not macrophages, lung epithelia or other endothelia. Phosphorylates the anti-anti-sigma factor homolog Rv0516c, which inhibits binding of Rv0516c to Rv2638, another anti-anti-sigma factor. Can also phosphorylate the FHA domain of Rv1747. {ECO:0000269|PubMed:15987910, ECO:0000269|PubMed:17242402, ECO:0000269|PubMed:17411339}.

Family

Protein kinase superfamily, Ser/Thr protein kinase family

GO

negative regulation of protein binding GO:0032091 biological_process
pathogenesis GO:0009405 biological_process
positive regulation of catalytic activity GO:0043085 biological_process
negative regulation of catalytic activity GO:0043086 biological_process
negative regulation of fatty acid biosynthetic process GO:0045717 biological_process
manganese ion binding GO:0030145 molecular_function
cell wall GO:0005618 cellular_component
extracellular region GO:0005576 cellular_component
cellular response to phosphate starvation GO:0016036 biological_process
protein kinase activity GO:0004672 molecular_function
protein serine/threonine kinase activity GO:0004674 molecular_function
plasma membrane GO:0005886 cellular_component
integral component of plasma membrane GO:0005887 cellular_component
cytosol GO:0005829 cellular_component
ATP binding GO:0005524 molecular_function

InterPro

UniProt: P9WI79
GenBank: Rv0931c
EnsemblBacteria: Rv0931c
Mycobrowser: Rv0931c

Summary

Name: Serine/threonine-protein kinase PknD (EC 2.7.11.1)
Family: Protein kinase superfamily, Ser/Thr protein kinase family
Protein Sequence: MSDAVPQVGSQFGPYQLLRLLGRGGMGEVYEAEDTRKHRVVALKLISPQYSDNAVFRARMQREADTAGRLTEPHIVPIHDYGEINGQFFVEMRMIDGTSLRALLKQYGPLTPARAVAIVRQIAAALDAAHANGVTHRDVKPENILVTASDFAYLVDFGIARAASDPGLTQTGTAVGTYNYMAPERFTGDEVTYRADIYALACVLGECLTGAPPYRADSVERLIAAHLMDPAPQPSQLRPGRVPPALDQVIAKGMAKNPAERFMSAGDLAIAAHDALTTSEQHQATTILRRGDNATLLATPADTGLSQSESGIAGAGTGPPTPGAARWSPGDSATVAGPLAADSRGGNWPSQTGHSPAVPNALQASLGHAVPPAGNKRKVWAVVGAAAIVLVAIVAAAGYLVLRPSWSPTQASGQTVLPFTGIDFRLSPSGVAVDSAGNVYVTSEGMYGRVVKLATGSTGTTVLPFNGLYQPQGLAVDGAGTVYVTDFNNRVVTLAAGSNNQTVLPFDGLNYPEGLAVDTQGAVYVADRGNNRVVKLAAGSKTQTVLPFTGLNDPDGVAVDNSGNVYVTDTDNNRVVKLEAESNNQVVLPFTDITAPWGIAVDEAGTVYVTEHNTNQVVKLLAGSTTSTVLPFTGLNTPLAVAVDSDRTVYVADRGNDRVVKLTS
Mass: 69,545 Da
Length: 664 Aa

Rv0931c doesn't seem to be a targeted by any drug.

Rv0931c doesn't seem to be involved in any pathway.

Sensor domain of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknD, forms a highly symmetric beta propeller.: J Mol Biol. 2004 May 28;339(2):459-69. doi: 10.1016/j.jmb.2004.03.063.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Effect of PstS sub-units or PknD deficiency on the survival of Mycobacterium tuberculosis.: Tuberculosis (Edinb). 2010 Nov;90(6):338-45. doi: 10.1016/j.tube.2010.09.004. Epub 2010 Oct 8.
M. tuberculosis Ser/Thr protein kinase D phosphorylates an anti-anti-sigma factor homolog.: PLoS Pathog. 2007 Apr;3(4):e49. doi: 10.1371/journal.ppat.0030049.
Allosteric activation by dimerization of the PknD receptor Ser/Thr protein kinase from Mycobacterium tuberculosis.: J Biol Chem. 2007 Apr 13;282(15):11427-35. doi: 10.1074/jbc.M610193200. Epub 2007 Jan 22.
Characterization of the phosphorylation sites of Mycobacterium tuberculosis serine/threonine protein kinases, PknA, PknD, PknE, and PknH by mass spectrometry.: Proteomics. 2006 Jul;6(13):3754-66. doi: 10.1002/pmic.200500900.
Mycobacterium tuberculosis serine/threonine kinases PknB, PknD, PknE, and PknF phosphorylate multiple FHA domains.: Protein Sci. 2005 Jul;14(7):1918-21. doi: 10.1110/ps.051413405.
Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases.: Biochem Biophys Res Commun. 2005 Aug 5;333(3):858-67. doi: 10.1016/j.bbrc.2005.05.173.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
A serine/threonine protein kinase from Mycobacterium tuberculosis.: Eur J Biochem. 1997 Mar 1;244(2):604-12. doi: 10.1111/j.1432-1033.1997.00604.x.