Function

With Ku forms a non-homologous end joining (NHEJ) repair enzyme which repairs DNA double-strand breaks (DSB) with reduced fidelity. Recognizes, processes and reseals DSBs, including repairs on incompatible DSB which require 3'-resection, gap filling and ligation. Anneals the 3' overhanging strands from opposing breaks to form a gapped intermediate, which then can be extended in trans by using the termini as primers for extension of the annealed break. Binds to the recessed 5'-phosphate moiety of the downstream DNA strand forming a stable synaptic complex even when the 3'-protruding ends of the template DNA strands are not complementary. Has numerous activites; gap filling copies the template strand, and prefers a 5'-phosphate in the gap and rNTPS (PubMed:17174332, PubMed:17947582), DNA-directed DNA or RNA polymerase on 5'-overhangs, terminal transferase (extending ssDNA or blunt dsDNA in a non-templated fashion, preferentially with rNTPs), DNA-dependent RNA primase (synthesizes short RNAs on unprimed closed ssDNA) and 3'- to 5'-exonuclease on ssDNA (PubMed:15499016). Isolated Pol domain (and presumably the holoenzyme) is able to form complexes between 2 noncompatible protruding 3'-ends DNA ends via microhomologous DNA strands, in a end-bridging function to which it adds a templated nucleotide (PubMed:17947582). Minimal primer length is 2 nucleotides (PubMed:21255731). {ECO:0000269|PubMed:15499016, ECO:0000269|PubMed:17174332, ECO:0000269|PubMed:17947582, ECO:0000269|PubMed:21255731}.; FUNCTION: The preference of the polymerase domain for rNTPs over dNTPs may be advantageous in dormant cells, where the dNTP pool is limiting.; FUNCTION: In conjunction with endogenous or Mycobacterium phage Omega Ku (AC Q853W0) can reconstitute NHEJ in Saccharomyces cerevisiae.

Family

LigD polymerase family; LigD 3'-phosphoesterase family; ATP-dependent DNA ligase family

GO

• metal ion binding GO:0046872 molecular_function
• ribonucleotide binding GO:0032553 molecular_function
• magnesium ion binding GO:0000287 molecular_function
• DNA ligase (ATP) activity GO:0003910 molecular_function
• DNA ligase activity GO:0003909 molecular_function
• DNA-directed 5'-3' RNA polymerase activity GO:0003899 molecular_function
• DNA primase activity GO:0003896 molecular_function
• DNA-directed DNA polymerase activity GO:0003887 molecular_function
• manganese ion binding GO:0030145 molecular_function
• ATP binding GO:0005524 molecular_function
• DNA binding GO:0003677 molecular_function
• DNA replication, synthesis of RNA primer GO:0006269 biological_process
• viral process GO:0016032 biological_process
• polynucleotide adenylyltransferase activity GO:0004652 molecular_function
• nucleotide binding GO:0000166 molecular_function
• single-stranded DNA 3'-5' exodeoxyribonuclease activity GO:0008310 molecular_function
• plasma membrane GO:0005886 cellular_component
• double-strand break repair via nonhomologous end joining GO:0006303 biological_process
• double-strand break repair GO:0006302 biological_process
• DNA recombination GO:0006310 biological_process
• DNA ligation GO:0006266 biological_process

InterPro

• IPR012309
• IPR012310
• IPR014146
• IPR014144
• IPR014145
• IPR033649
• IPR012340

UniProt

P9WNV3

GenBank

Rv0938

EnsemblBacteria

Rv0938

Mycobrowser

Rv0938

Rv0938 doesn't seem to be a targeted by any drug.

Rv0938 doesn't seem to be involved in any pathway.