Gene - combatTB X

Rv0998

Type: protein_coding
Name: Rv0998
Locus Name: Rv0998
Product: Conserved hypothetical protein
Functional Category: Conserved hypotheticals
Location: 1114748..1115749 (+ strand)
Gene Length: 1001 bp
Nucleotides: TGGACGGGATAGCCGAATTGACCGGCGCACGAGTCGAAGATCTTGCGGGGATGGACGTCTTTCAGGGATGTCCGGCCGAGGGTCTGGTGTCATTGGCGGCGAGCGTTCAGCCGTTGCGGGCCGCTGCCGGCCAGGTGCTGCTGCGGCAGGGCGAGCCGGCGGTTTCGTTTCTGCTTATCTCGTCGGGTAGCGCAGAAGTCAGCCATGTTGGCGACGATGGTGTTGCGATCATCGCTCGGGCGCTGCCGGGCATGATCGTCGGCGAAATCGCGCTGCTGCGCGATAGCCCGCGCAGCGCGACGGTCACCACCATCGAGCCGCTGACCGGCTGGACGGGTGGCCGCGGCGCTTTCGCCACAATGGTGCACATCCCCGGGGTCGGTGAGCGATTGCTGCGCACCGCCAGGCAGCGTCTCGCCGCCTTCGTCTCCCCGATTCCGGTACGGCTTGCCGACGGGACTCAACTGATGCTACGCCCCGTGCTGCCCGGTGACCGCGAGCGGACCGTGCACGGACACATCCAGTTCTCCGGCGAGACGCTGTATCGACGGTTCATGTCGGCTCGTGTTCCCAGTCCGGCGTTGATGCACTACCTGTCGGAAGTCGACTACGTCGACCACTTCGTCTGGGTGGTGACCGACGGAAGCGACCCCGTAGCCGACGCGCGTTTTGTGCGGGATGAAACCGATCCGACGGTCGCCGAGATCGCGTTCACGGTTGCCGACGCGTATCAGGGCAGGGGGATTGGAAGCTTTCTCATCGGTGCGTTGTCCGTGGCCGCCCGGGTCGACGGCGTCGAAAGGTTTGCCGCGCGCATGCTTTCCGACAATGTGCCGATGCGAACGATCATGGACCGCTACGGGGCGGTGTGGCAGCGCGAGGACGTCGGAGTCATCACCACCATGATCGATGTGCCGGGTCCGGGTGAGCTGAGCTTGGGGCGCGAGATGGTCGACCAGATCAACCGGGTAGCCCGGCAAGTGATCGAGGCCGTCGGCTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes specifically the acetylation of the epsilon-amino group of a highly conserved lysine residue in acetyl-CoA synthetase (ACS). This acetylation results in the inactivation of ACS activity and could be important for mycobacteria to adjust to environmental changes. {ECO:0000269|PubMed:20507997, ECO:0000269|PubMed:21627103, ECO:0000269|PubMed:22773105}.

Family

Unknown

GO

transferase activity, transferring acyl groups GO:0016746 molecular_function
acetyltransferase activity GO:0016407 molecular_function
cytosol GO:0005829 cellular_component
cAMP binding GO:0030552 molecular_function
DNA-binding transcription factor activity GO:0003700 molecular_function
metal ion binding GO:0046872 molecular_function

InterPro

UniProt: O05581
GenBank: Rv0998
EnsemblBacteria: Rv0998
Mycobrowser: Rv0998

Summary

Name: Acetyltransferase Pat (EC 2.3.1.-) (GCN5-like enzyme) (GCN5-related N-acetyltransferase) (GNAT) (Protein acetyltransferase) (Pat)
Family: Unknown
Protein Sequence: MDGIAELTGARVEDLAGMDVFQGCPAEGLVSLAASVQPLRAAAGQVLLRQGEPAVSFLLISSGSAEVSHVGDDGVAIIARALPGMIVGEIALLRDSPRSATVTTIEPLTGWTGGRGAFATMVHIPGVGERLLRTARQRLAAFVSPIPVRLADGTQLMLRPVLPGDRERTVHGHIQFSGETLYRRFMSARVPSPALMHYLSEVDYVDHFVWVVTDGSDPVADARFVRDETDPTVAEIAFTVADAYQGRGIGSFLIGALSVAARVDGVERFAARMLSDNVPMRTIMDRYGAVWQREDVGVITTMIDVPGPGELSLGREMVDQINRVARQVIEAVG
Mass: 35,626 Da
Length: 333 Aa

Rv0998 doesn't seem to be a targeted by any drug.

Rv0998 doesn't seem to be involved in any pathway.

Cyclic AMP regulation of protein lysine acetylation in Mycobacterium tuberculosis.: Nat Struct Mol Biol. 2012 Aug;19(8):811-8. doi: 10.1038/nsmb.2318. Epub 2012 Jul 8.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Reversible acetylation and inactivation of Mycobacterium tuberculosis acetyl-CoA synthetase is dependent on cAMP.: Biochemistry. 2011 Jul 5;50(26):5883-92. doi: 10.1021/bi200156t. Epub 2011 Jun 10.
cAMP-regulated protein lysine acetylases in mycobacteria.: J Biol Chem. 2010 Aug 6;285(32):24313-23. doi: 10.1074/jbc.M110.118398. Epub 2010 May 27.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.