Gene - combatTB X

mfd

Type: protein_coding
Name: mfd
Locus Name: Rv1020
Product: Probable transcription-repair coupling factor Mfd (TRCF)
Functional Category: Information pathways
Location: 1138967..1142671 (+ strand)
Gene Length: 3704 bp
Nucleotides: TGACCGCACCGGGGCCTGCCTGCTCAGATACCCCGATCGCGGGGCTCGTCGAATTGGCGCTGAGCGCGCCGACATTCCAACAGCTCATGCAGCGCGCCGGGGGTCGACCCGACGAATTGACGCTCATCGCGCCGGCCAGCGCGCGGCTGTTGGTCGCCAGTGCGCTGGCTCGGCAGGGGCCATTGCTGGTGGTCACCGCCACCGGGCGGGAAGCCGACGACCTGGCCGCCGAACTGCGTGGTGTGTTCGGGGATGCGGTGGCGTTGTTGCCGTCCTGGGAGACACTGCCGCACGAACGGCTCTCACCCGGTGTTGACACCGTCGGCACTCGCCTGATGGCGCTGCGCCGGCTGGCCCACCCCGACGATGCCCAGCTGGGCCCACCGCTGGGGGTAGTGGTGACCTCGGTGCGCTCGCTGCTGCAGCCCATGACGCCGCAGCTGGGCATGATGGAGCCCCTCACGCTGACCGTTGGCGACGAATCCCCCTTCGACGGCGTGGTGGCGCGGCTGGTCGAGCTGGCATATACCCGGGTGGATATGGTCGGCCGGCGCGGCGAGTTCGCTGTGCGCGGCGGGATTCTGGACATCTTTGCCCCGACGGCCGAACATCCGGTGCGGGTCGAGTTCTGGGGCGACGAGATCACCGAGATGCGGATGTTCTCGGTAGCCGACCAGCGCTCGATTCCGGAGATCGACATTCACACACTGGTTGCCTTCGCCTGCCGTGAACTGCTGCTGAGCGAGGACGTGCGGGCGCGGGCCGCCCAACTGGCCGCACGGCATCCCGCGGCCGAGAGCACCGTCACCGGCAGTGCTTCCGACATGCTGGCGAAGCTCGCCGAGGGCATCGCGGTCGACGGCATGGAGGCGGTGTTGCCGGTGCTCTGGTCCGACGGGCACGCGTTGCTGACCGATCAGCTGCCCGACGGCACGCCGGTGTTGGTGTGCGACCCGGAAAAGGTGCGCACCCGCGCCGCGGATCTGATCAGGACTGGCCGTGAATTCCTGGAAGCCTCGTGGTCGGTCGCGGCGCTGGGAACTGCAGAAAATCAAGCCCCCGTCGACGTCGAACAACTGGGTGGGTCGGGGTTCGTCGAACTGGACCAGGTGCGGGCCGCGGCGGCCCGAACGGGTCATCCGTGGTGGACGTTGAGCCAATTGTCCGACGAGTCGGCGATCGAGTTGGACGTTCGGGCCGCGCCGTCGGCGCGCGGGCACCAGCGTGACATCGACGAAATCTTCGCGATGCTACGTGCCCACATCGCGACCGGCGGGTACGCCGCGCTGGTCGCGCCGGGCACCGGAACCGCACACCGCGTGGTGGAACGGCTGTCCGAGTCCGACACCCCCGCGGGGATGCTCGATCCCGGCCAGGCGCCCAAGCCGGGAGTCGTCGGGGTGCTCCAGGGCCCGCTGCGTGACGGCGTCATCATTCCCGGCGCCAACCTGGTCGTCATCACCGAGACCGATTTGACCGGCAGCCGGGTCAGCGCCGCCGAGGGCAAGCGGCTGGCGGCCAAGCGGCGCAACATCGTCGACCCGCTGGCGCTGACGGCCGGTGACCTGGTGGTGCACGATCAGCACGGCATCGGCCGGTTCGTGGAGATGGTCGAGCGCACGGTCGGGGGCGCCCGCCGGGAGTATCTGGTGCTGGAGTATGCCTCGGCCAAGAGGGGTGGCGGGGCGAAAAATACTGACAAGCTCTATGTCCCGATGGATTCGCTGGACCAGCTGTCGCGGTATGTCGGCGGGCAGGCGCCGGCGCTGAGCCGGCTGGGCGGCAGCGACTGGGCCAACACCAAGACCAAGGCGCGCCGCGCGGTGCGCGAGATCGCGGGCGAGCTGGTCTCGCTGTACGCCAAACGGCAGGCCAGCCCCGGGCATGCGTTCTCGCCGGACACGCCGTGGCAGGCCGAGCTGGAGGACGCGTTCGGCTTCACCGAGACCGTGGACCAGCTCACCGCCATCGAAGAGGTCAAGGCGGACATGGAAAAGCCGATCCCGATGGACCGGGTGATCTGCGGCGATGTCGGCTACGGCAAGACCGAGATCGCGGTGCGGGCGGCGTTCAAGGCGGTCCAAGACGGTAAACAGGTCGCGGTGCTGGTGCCCACCACGCTGCTGGCCGACCAGCATCTGCAGACGTTCGGCGAGCGAATGTCCGGATTCCCGGTGACCATCAAGGGTCTGTCGCGGTTCACCGACGCCGCCGAGTCCCGCGCCGTGATCGACGGCCTGGCCGACGGGTCGGTGGACATCGTGATCGGCACCCATCGGCTGCTGCAGACCGGGGTGCGCTGGAAGGATCTGGGCCTGGTGGTGGTCGACGAGGAGCAGCGGTTCGGCGTCGAGCACAAGGAGCACATCAAGTCACTGCGCACCCATGTCGACGTGCTGACCATGAGCGCCACCCCGATCCCGCGCACGTTGGAGATGAGCCTGGCCGGGATTCGCGAGATGTCGACCATCCTGACGCCGCCCGAGGAGCGCTACCCGGTGCTGACCTACGTCGGACCGCACGACGACAAGCAGATCGCCGCGGCGCTGCGCCGGGAGCTGCTGCGCGACGGGCAGGCGTTCTACGTGCACAACCGGGTCAGCTCGATCGACGCGGCCGCCGCCCGGGTGCGTGAGCTGGTGCCCGAGGCGCGGGTGGTGGTCGCGCACGGGCAGATGCCCGAGGACCTGTTGGAGACCACCGTGCAACGGTTCTGGAACCGCGAGCATGACATCCTGGTTTGCACCACCATCGTGGAGACCGGCCTGGACATCTCCAACGCCAACACTTTGATCGTCGAGCGCGCCGATACCTTCGGGCTGTCCCAGCTGCACCAGCTGCGTGGCCGGGTGGGCCGCAGCCGGGAGCGCGGCTACGCCTATTTCCTCTATCCACCGCAGGTGCCGCTGACCGAGACCGCTTACGACCGGTTGGCGACGATCGCGCAGAACAATGAGCTGGGCGCGGGCATGGCCGTGGCGTTGAAGGACCTAGAGATCCGCGGTGCCGGCAACGTGCTCGGCATCGAGCAGTCCGGACACGTCGCCGGCGTCGGATTCGACCTGTACGTGCGGTTGGTCGGCGAGGCCCTGGAGACGTACCGGGACGCGTACCGGGCGGCCGCCGACGGCCAAACCGTGAGGACCGCCGAAGAACCCAAGGATGTGCGAATCGACCTGCCCGTTGACGCGCACCTGCCACCGGACTACATCGCCAGTGATCGGCTGCGGCTGGAGGGCTACCGGCGGCTGGCGGCCGCCTCCTCTGATCGCGAAGTGGCGGCCGTTGTGGACGAGCTAACCGATCGGTATGGGGCCCTGCCGGAGCCGGCCCGGCGGCTGGCGGCGGTGGCACGGCTGCGGCTGCTGTGCCGTGGCTCCGGCATCACCGACGTGACGGCGGCGTCGGCAGCGACCGTGCGGCTGTCCCCGTTGACGCTGCCGGACTCCGCCCAGGTGCGGCTGAAGCGAATGTATCCCGGAGCGCACTACCGTGCCACGACGGCCACCGTGCAGGTTCCCATTCCGCGAGCCGGTGGCCTCGGCGCGCCGCGAATCCGCGACGTCGAGCTGGTTCAGATGGTGGCCGATTTGATAACCGCGCTCGCTGGGAAACCGCGCCAGCATATTGGTATAACGAACCCTAGCCCGCCAGGCGAAGACGGCCGTGGTCGCAACACGACGATTAAGGAGCGACAACCGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site. {ECO:0000255|HAMAP-Rule:MF_00969}.

Family

UvrB family; Helicase family, RecG subfamily

GO

transcription-coupled nucleotide-excision repair, DNA damage recognition GO:0000716 biological_process
ATP binding GO:0005524 molecular_function
cytosol GO:0005829 cellular_component
damaged DNA binding GO:0003684 molecular_function
regulation of transcription, DNA-templated GO:0006355 biological_process
helicase activity GO:0004386 molecular_function
plasma membrane GO:0005886 cellular_component

InterPro

UniProt: P9WMQ5
GenBank: Rv1020
EnsemblBacteria: Rv1020
Mycobrowser: Rv1020

Summary

Name: Transcription-repair-coupling factor (TRCF) (EC 3.6.4.-)
Family: UvrB family; Helicase family, RecG subfamily
Protein Sequence: MTAPGPACSDTPIAGLVELALSAPTFQQLMQRAGGRPDELTLIAPASARLLVASALARQGPLLVVTATGREADDLAAELRGVFGDAVALLPSWETLPHERLSPGVDTVGTRLMALRRLAHPDDAQLGPPLGVVVTSVRSLLQPMTPQLGMMEPLTLTVGDESPFDGVVARLVELAYTRVDMVGRRGEFAVRGGILDIFAPTAEHPVRVEFWGDEITEMRMFSVADQRSIPEIDIHTLVAFACRELLLSEDVRARAAQLAARHPAAESTVTGSASDMLAKLAEGIAVDGMEAVLPVLWSDGHALLTDQLPDGTPVLVCDPEKVRTRAADLIRTGREFLEASWSVAALGTAENQAPVDVEQLGGSGFVELDQVRAAAARTGHPWWTLSQLSDESAIELDVRAAPSARGHQRDIDEIFAMLRAHIATGGYAALVAPGTGTAHRVVERLSESDTPAGMLDPGQAPKPGVVGVLQGPLRDGVIIPGANLVVITETDLTGSRVSAAEGKRLAAKRRNIVDPLALTAGDLVVHDQHGIGRFVEMVERTVGGARREYLVLEYASAKRGGGAKNTDKLYVPMDSLDQLSRYVGGQAPALSRLGGSDWANTKTKARRAVREIAGELVSLYAKRQASPGHAFSPDTPWQAELEDAFGFTETVDQLTAIEEVKADMEKPIPMDRVICGDVGYGKTEIAVRAAFKAVQDGKQVAVLVPTTLLADQHLQTFGERMSGFPVTIKGLSRFTDAAESRAVIDGLADGSVDIVIGTHRLLQTGVRWKDLGLVVVDEEQRFGVEHKEHIKSLRTHVDVLTMSATPIPRTLEMSLAGIREMSTILTPPEERYPVLTYVGPHDDKQIAAALRRELLRDGQAFYVHNRVSSIDAAAARVRELVPEARVVVAHGQMPEDLLETTVQRFWNREHDILVCTTIVETGLDISNANTLIVERADTFGLSQLHQLRGRVGRSRERGYAYFLYPPQVPLTETAYDRLATIAQNNELGAGMAVALKDLEIRGAGNVLGIEQSGHVAGVGFDLYVRLVGEALETYRDAYRAAADGQTVRTAEEPKDVRIDLPVDAHLPPDYIASDRLRLEGYRRLAAASSDREVAAVVDELTDRYGALPEPARRLAAVARLRLLCRGSGITDVTAASAATVRLSPLTLPDSAQVRLKRMYPGAHYRATTATVQVPIPRAGGLGAPRIRDVELVQMVADLITALAGKPRQHIGITNPSPPGEDGRGRNTTIKERQP
Mass: 132,908 Da
Length: 1234 Aa

Rv1020 doesn't seem to be a targeted by any drug.

Nucleotide excision repair mtu03420
mtu03420
Genetic Information Processing; Replication and repair

Interaction of CarD with RNA polymerase mediates Mycobacterium tuberculosis viability, rifampin resistance, and pathogenesis.: J Bacteriol. 2012 Oct;194(20):5621-31. doi: 10.1128/JB.00879-12. Epub 2012 Aug 17.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.