Gene - combatTB X

pknH

Type: protein_coding
Name: pknH
Locus Name: Rv1266c
Product: Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H)
Functional Category: Regulatory proteins
Location: 1413960..1415840 (- strand)
Gene Length: 1880 bp
Nucleotides: ATGAGCGACGCACAGGACTCGCGGGTGGGCTCGATGTTTGGGCCCTACCACCTCAAACGGCTGTTGGGCCGGGGCGGGATGGGCGAGGTCTACGAGGCCGAGCACACCGTCAAGGAGTGGACCGTCGCCGTCAAGCTGATGACCGCGGAATTCAGCAAGGACCCGGTGTTTCGCGAGCGGATGAAACGCGAAGCCCGCATCGCCGGCCGGTTGCAGGAACCTCACGTGGTGCCCATCCACGACTACGGCGAAGTCGACGGCCAAATGTTTTTGGAGATGCGCCTGGTGGAGGGCACCGACCTTGACAGCGTGCTCAAACGCTTCGGCCCGCTGACCCCACCGCGCGCGGTGGCCATCATCACCCAGATCGCCTCGGCGCTCGATGCCGCACACGCCGACGGGGTGATGCACCGCGACGTCAAACCGCAAAACATTCTGATCACCCGCGACGACTTTGCCTATCTGGTCGACTTCGGGATCGCCAGTGCGACCACCGACGAGAAACTGACCCAGTTAGGCACCGCGGTGGGCACCTGGAAATACATGGCCCCCGAACGGTTCTCCAACGACGAGGTAACCTACCGCGCCGACATCTACGCGCTGGCCTGCGTGCTGCACGAATGCTTGACCGGGGCCCCGCCGTATCGCGCCGACAGCGCCGGCACACTGGTCAGCTCCCACCTGATGGGACCCATCCCCCAGCCCAGCGCCATCCGCCCGGGCATCCCCAAAGCCTTTGACGCGGTGGTCGCCCGCGGCATGGCCAAAAAGCCCGAAGACCGCTACGCCAGCGCCGGCGACCTGGCCCTGGCCGCCCACGAAGCGCTCAGCGACCCCGACCAAGACCACGCCGCCGACATCCTGCGCCGCAGCCAAGAATCCACCCTGCCCGCGCCGCCTAAGCCCGTCCCGCCTCCGACCATGCCGGCCACCGCCATGGCTCCTCGGCAGCCACCCGCACCCCCCGTCACCCCGCCGGGTGTCCAGCCGGCACCAAAGCCGTCCTACACGCCCCCGGCCCAGCCGGGGCCTGCCGGACAGCGGCCTGGCCCCACCGGCCAGCCGTCCTGGGCGCCCAATAGCGGCCCGATGCCGGCCAGCGGACCCACTCCCACCCCGCAGTACTACCAGGGCGGCGGCTGGGGCGCACCGCCGTCCGGCGGCCCCAGCCCCTGGGCCCAGACCCCGCGCAAAACCAACCCGTGGCCCTTAGTGGCCGGCGCCGCCGCCGTCGTGCTCGTCCTCGTGTTGGGCGCCATCGGCATCTGGATCGCCATCCGGCCCAAGCCGGTACAGCCGCCTCAGCCGGTTGCGGAGGAGCGCCTTAGCGCCCTACTGCTGAACTCCTCAGAAGTCAACGCCGTGATGGGCTCGTCGTCCATGCAGCCGGGCAAACCGATCACATCGATGGACTCTTCGCCGGTGACGGTGTCCCTGCCGGACTGCCAGGGCGCGCTGTATACCAGCCAGGATCCGGTGTATGCCGGCACCGGCTACACCGCCATCAACGGCTTGATTTCATCCGAGCCGGGCGACAACTACGAACATTGGGTGAACCAAGCCGTCGTCGCCTTTCCGACCGCCGACAAAGCCCGCGCGTTCGTGCAGACTTCGGCCGACAAATGGAAGAACTGCGCAGGCAAGACGGTCACCGTCACGAATAAGGCCAAGACCTACCGGTGGACGTTTGCCGACGTCAAAGGCAGCCCGCCGACGATCACGGTGATAGACACCCAAGAAGGCGCTGAGGGCTGGGAATGCCAACGCGCGATGAGCGTGGCCAACAATGTGGTCGTCGACGTCAACGCATGCGGGTACCGGATCACCAATCAAGCAGGCCAGATCGCCGCCAAGATCGTTGACAAAGTCAACAAGGAATG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

May regulate bacterial growth in response to external signals to facilitate adaptation to the host environment. In vitro, phosphorylates several substrates such as EmbR, DevR (DosR), DacB1 and Rv0681. {ECO:0000269|PubMed:14690440, ECO:0000269|PubMed:15043876, ECO:0000269|PubMed:16077122, ECO:0000269|PubMed:16585755, ECO:0000269|PubMed:16817899, ECO:0000269|PubMed:17286964, ECO:0000269|PubMed:20630871}.

Family

Protein kinase superfamily, Ser/Thr protein kinase family

GO

protein kinase activity GO:0004672 molecular_function
protein serine/threonine kinase activity GO:0004674 molecular_function
positive regulation of DNA binding GO:0043388 biological_process
negative regulation of growth GO:0045926 biological_process
positive regulation of transcription, DNA-templated GO:0045893 biological_process
response to host immune response GO:0052572 biological_process
integral component of plasma membrane GO:0005887 cellular_component
ATP binding GO:0005524 molecular_function
cell wall GO:0005618 cellular_component
negative regulation of catalytic activity GO:0043086 biological_process
positive regulation of catalytic activity GO:0043085 biological_process
protein autophosphorylation GO:0046777 biological_process
regulation of lipid biosynthetic process GO:0046890 biological_process

InterPro

UniProt: P9WI71
GenBank: Rv1266c
EnsemblBacteria: Rv1266c
Mycobrowser: Rv1266c

Summary

Name: Serine/threonine-protein kinase PknH (EC 2.7.11.1)
Family: Protein kinase superfamily, Ser/Thr protein kinase family
Protein Sequence: MSDAQDSRVGSMFGPYHLKRLLGRGGMGEVYEAEHTVKEWTVAVKLMTAEFSKDPVFRERMKREARIAGRLQEPHVVPIHDYGEVDGQMFLEMRLVEGTDLDSVLKRFGPLTPPRAVAIITQIASALDAAHADGVMHRDVKPQNILITRDDFAYLVDFGIASATTDEKLTQLGTAVGTWKYMAPERFSNDEVTYRADIYALACVLHECLTGAPPYRADSAGTLVSSHLMGPIPQPSAIRPGIPKAFDAVVARGMAKKPEDRYASAGDLALAAHEALSDPDQDHAADILRRSQESTLPAPPKPVPPPTMPATAMAPRQPPAPPVTPPGVQPAPKPSYTPPAQPGPAGQRPGPTGQPSWAPNSGPMPASGPTPTPQYYQGGGWGAPPSGGPSPWAQTPRKTNPWPLVAGAAAVVLVLVLGAIGIWIAIRPKPVQPPQPVAEERLSALLLNSSEVNAVMGSSSMQPGKPITSMDSSPVTVSLPDCQGALYTSQDPVYAGTGYTAINGLISSEPGDNYEHWVNQAVVAFPTADKARAFVQTSADKWKNCAGKTVTVTNKAKTYRWTFADVKGSPPTITVIDTQEGAEGWECQRAMSVANNVVVDVNACGYRITNQAGQIAAKIVDKVNKE
Mass: 66,754 Da
Length: 626 Aa

Rv1266c doesn't seem to be a targeted by any drug.

Rv1266c doesn't seem to be involved in any pathway.

Structure of the sensor domain of Mycobacterium tuberculosis PknH receptor kinase reveals a conserved binding cleft.: J Mol Biol. 2012 Sep 28;422(4):488-94. doi: 10.1016/j.jmb.2012.06.011. Epub 2012 Jun 20.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Convergence of Ser/Thr and two-component signaling to coordinate expression of the dormancy regulon in Mycobacterium tuberculosis.: J Biol Chem. 2010 Sep 17;285(38):29239-46. doi: 10.1074/jbc.M110.132894. Epub 2010 Jul 14.
Novel substrates of Mycobacterium tuberculosis PknH Ser/Thr kinase.: Biochem Biophys Res Commun. 2007 Mar 30;355(1):162-8. doi: 10.1016/j.bbrc.2007.01.122. Epub 2007 Jan 30.
Characterization of the phosphorylation sites of Mycobacterium tuberculosis serine/threonine protein kinases, PknA, PknD, PknE, and PknH by mass spectrometry.: Proteomics. 2006 Jul;6(13):3754-66. doi: 10.1002/pmic.200500900.
Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo.: J Bacteriol. 2006 Apr;188(8):2936-44. doi: 10.1128/JB.188.8.2936-2944.2006.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
An FHA phosphoprotein recognition domain mediates protein EmbR phosphorylation by PknH, a Ser/Thr protein kinase from Mycobacterium tuberculosis.: Biochemistry. 2003 Dec 30;42(51):15300-9. doi: 10.1021/bi035150b.
PknH, a transmembrane Hank's type serine/threonine kinase from Mycobacterium tuberculosis is differentially expressed under stress conditions.: FEMS Microbiol Lett. 2004 Apr 1;233(1):107-13. doi: 10.1016/j.femsle.2004.01.045.
Deletion of the Mycobacterium tuberculosis pknH gene confers a higher bacillary load during the chronic phase of infection in BALB/c mice.: J Bacteriol. 2005 Aug;187(16):5751-60. doi: 10.1128/JB.187.16.5751-5760.2005.
EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine/threonine kinases and phosphatase in Mycobacterium tuberculosis.: FEBS J. 2006 Jun;273(12):2711-21. doi: 10.1111/j.1742-4658.2006.05289.x.