Gene - combatTB X

cysN

Type: protein_coding
Name: cysN
Locus Name: Rv1286
Product: Probable bifunctional enzyme CysN/CysC: sulfate adenyltransferase (subunit 1) + adenylylsulfate kinase
Functional Category: Intermediary metabolism and respiration
Location: 1438907..1440751 (+ strand)
Gene Length: 1844 bp
Nucleotides: TGACGACGCTATTGCGGCTGGCGACAGCGGGTTCCGTCGACGATGGCAAGTCCACGCTGATTGGGCGGCTACTCTACGACTCCAAGGCTGTGATGGAAGACCAGTGGGCGTCGGTGGAGCAAACGTCCAAGGACCGGGGCCACGACTACACCGACCTGGCTCTGGTCACCGACGGCCTGCGGGCCGAGCGGGAACAGGGCATCACCATCGACGTTGCCTACCGCTACTTCGCCACTCCCAAGCGGAAATTCATCATTGCCGACACCCCGGGACACATCCAATACACCCGCAACATGGTGACCGGTGCGTCCACCGCCCAACTGGTGATCGTACTGGTGGATGCCCGGCACGGCTTGCTGGAGCAATCCCGCCGGCACGCCTTCCTGGCGTCGCTGCTGGGCATCCGCCACCTGGTGCTCGCGGTCAACAAGATGGACTTGCTTGGCTGGGACCAAGAGAAATTCGACGCGATTCGAGACGAATTCCACGCCTTCGCGGCCCGCCTCGACGTGCAGGACGTCACCTCCATCCCAATCTCCGCGCTGCACGGCGACAACGTGGTGACCAAATCCGACCAGACGCCCTGGTACGAGGGACCGTCGCTGCTGTCGCATCTCGAAGACGTCTACATCGCCGGTGACCGCAACATGGTCGACGTGCGATTCCCGGTCCAGTACGTCATCCGGCCGCACACCCTCGAGCATCAAGACCACCGCAGCTACGCGGGCACCGTGGCCAGTGGGGTAATGCGTTCAGGCGACGAAGTTGTCGTGCTGCCGATCGGTAAGACCACCCGGATCACCGCGATCGACGGCCCGAACGGCCCGGTGGCAGAAGCGTTTCCGCCGATGGCGGTTTCGGTGCGGCTCGCCGACGACATCGATATCTCGCGTGGTGACATGATCGCTCGCACCCACAACCAGCCCAGGATCACACAAGAATTCGACGCGACCGTGTGCTGGATGGCCGACAACGCGGTGCTAGAGCCCGGCCGCGACTACGTTGTCAAGCACACCACCCGAACCGTCCGCGCGAGGATAGCCGGGCTGGATTACCGGCTCGATGTCAACACCCTGCATCGCGACAAGACCGCAACGGCGTTGAAACTCAACGAACTGGGCCGTGTTTCGCTGCGCACCCAGGTGCCGTTGCTGCTTGACGAGTACACCCGCAACGCTAGCACCGGCTCGTTCATCCTCATTGACCCCGACACCAACGGAACGGTGGCGGCGGGCATGGTGTTACGCGACGTCTCGGCCCGCACGCCTAGCCCGAACACGGTGCGGCACAGATCGCTCGTCACTGCGCAAGATCGGCCGCCCAGGGGCAAGACGGTGTGGTTTACCGGACTGTCCGGCTCCGGCAAGTCGTCGGTGGCCATGCTGGTTGAGCGGAAGCTACTCGAAAAGGGCATCTCCGCTTACGTTCTGGACGGCGACAACCTACGGCATGGCCTCAACGCCGACCTGGGCTTTTCCATGGCCGACCGCGCGGAGAACCTGCGCCGGCTGTCGCATGTGGCCACACTGCTCGCCGATTGTGGCCACCTGGTGCTGGTGCCCGCGATCAGCCCCCTTGCTGAGCACCGTGCCCTGGCTCGTAAAGTGCACGCTGATGCGGGAATCGACTTTTTCGAGGTGTTCTGTGACACCCCGCTGCAGGACTGTGAGAGGCGTGATCCCAAAGGGTTGTACGCCAAAGCGCGTGCGGGTGAGATCACGCACTTCACCGGGATCGACAGCCCATATCAGCGGCCCAAGAACCCAGACCTACGGCTTACGCCGGATCGCAGCATAGACGAGCAGGCGCAGGAGGTTATCGACCTGTTGGAGTCATCGTCTTAG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity. {ECO:0000250}.; FUNCTION: APS kinase catalyzes the synthesis of activated sulfate. {ECO:0000250}.

Family

APS kinase family; TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, CysN/NodQ subfamily

GO

cellular response to oxidative stress GO:0034599 biological_process
cellular response to sulfur starvation GO:0010438 biological_process
sulfate assimilation via adenylyl sulfate reduction GO:0010134 biological_process
sulfate adenylyltransferase complex (ATP) GO:0009336 cellular_component
GTPase activity GO:0003924 molecular_function
hydrogen sulfide biosynthetic process GO:0070814 biological_process
ATP binding GO:0005524 molecular_function
GTP binding GO:0005525 molecular_function
cytosol GO:0005829 cellular_component
plasma membrane GO:0005886 cellular_component
sulfate assimilation GO:0000103 biological_process
sulfate adenylyltransferase (ATP) activity GO:0004781 molecular_function
adenylylsulfate kinase activity GO:0004020 molecular_function

InterPro

UniProt: P9WNM5
GenBank: Rv1286
EnsemblBacteria: Rv1286
Mycobrowser: Rv1286

Summary

Name: Bifunctional enzyme CysN/CysC [Includes: Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) (ATP-sulfurylase large subunit) (Sulfate adenylate transferase) (SAT); Adenylyl-sulfate kinase (EC 2.7.1.25) (APS kinase) (ATP adenosine-5'-phosphosulfate 3'-phosphotransferase)]
Family: APS kinase family; TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, CysN/NodQ subfamily
Protein Sequence: MTTLLRLATAGSVDDGKSTLIGRLLYDSKAVMEDQWASVEQTSKDRGHDYTDLALVTDGLRAEREQGITIDVAYRYFATPKRKFIIADTPGHIQYTRNMVTGASTAQLVIVLVDARHGLLEQSRRHAFLASLLGIRHLVLAVNKMDLLGWDQEKFDAIRDEFHAFAARLDVQDVTSIPISALHGDNVVTKSDQTPWYEGPSLLSHLEDVYIAGDRNMVDVRFPVQYVIRPHTLEHQDHRSYAGTVASGVMRSGDEVVVLPIGKTTRITAIDGPNGPVAEAFPPMAVSVRLADDIDISRGDMIARTHNQPRITQEFDATVCWMADNAVLEPGRDYVVKHTTRTVRARIAGLDYRLDVNTLHRDKTATALKLNELGRVSLRTQVPLLLDEYTRNASTGSFILIDPDTNGTVAAGMVLRDVSARTPSPNTVRHRSLVTAQDRPPRGKTVWFTGLSGSGKSSVAMLVERKLLEKGISAYVLDGDNLRHGLNADLGFSMADRAENLRRLSHVATLLADCGHLVLVPAISPLAEHRALARKVHADAGIDFFEVFCDTPLQDCERRDPKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRSIDEQAQEVIDLLESSS
Mass: 67,839 Da
Length: 614 Aa

Rv1286 doesn't seem to be a targeted by any drug.

Sulfate assimilation R-MTU-936635
Mycobacterium tuberculosis
R-MTU-936635

Sulfur metabolism mtu00920
mtu00920
Metabolism; Energy metabolism

Selenocompound metabolism mtu00450
mtu00450
PATHWAY_MAP mtu00450 Selenocompound metabolism

Monobactam biosynthesis mtu00261
mtu00261
Metabolism; Biosynthesis of other secondary metabolites

Purine metabolism mtu00230
mtu00230
PATHWAY_MAP mtu00230 Purine metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
The Mycobacterium tuberculosis cysD and cysNC genes form a stress-induced operon that encodes a tri-functional sulfate-activating complex.: Microbiology. 2004 Jun;150(Pt 6):1681-6. doi: 10.1099/mic.0.26894-0.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.