Gene - combatTB X

lysA

Type: protein_coding
Name: lysA
Locus Name: Rv1293
Product: Diaminopimelate decarboxylase LysA (DAP decarboxylase)
Functional Category: Intermediary metabolism and respiration
Location: 1448028..1449371 (+ strand)
Gene Length: 1343 bp
Nucleotides: TGAACGAGCTGCTGCACTTAGCGCCGAATGTGTGGCCGCGCAATACTACTCGCGATGAAGTCGGTGTGGTCTGCATCGCAGGAATTCCACTGACGCAGCTCGCCCAGGAGTACGGGACCCCGCTGTTCGTCATCGACGAGGACGACTTTCGCTCGCGCTGCCGAGAAACCGCCGCGGCCTTTGGAAGTGGGGCGAACGTGCACTATGCCGCCAAGGCGTTCCTGTGCAGCGAAGTAGCCCGGTGGATCAGCGAAGAAGGGCTCTGTCTGGACGTTTGCACCGGTGGGGAGTTGGCGGTCGCGCTGCACGCTAGCTTTCCGCCCGAGCGAATTACCTTGCACGGCAACAACAAATCGGTCTCAGAGTTGACCGCTGCGGTCAAAGCCGGAGTCGGCCATATTGTCGTCGATTCGATGACCGAGATCGAGCGCCTCGACGCCATCGCGGGCGAGGCCGGAATCGTCCAGGATGTCCTGGTGCGTCTCACCGTCGGTGTCGAGGCGCACACCCACGAGTTCATCTCCACCGCGCACGAGGACCAGAAATTCGGGTTATCGGTGGCCAGCGGCGCGGCCATGGCAGCGGTGCGGCGCGTTTTCGCCACTGATCACCTGCGCCTGGTTGGGCTACACAGCCACATCGGTTCGCAGATCTTCGACGTGGACGGCTTCGAACTCGCCGCGCACCGTGTCATCGGCCTGCTACGCGACGTCGTCGGCGAGTTCGGTCCCGAAAAGACGGCACAGATCGCGACCGTCGATCTCGGTGGCGGCTTGGGCATCTCGTATTTGCCGTCCGACGACCCACCGCCGATAGCCGAGCTCGCGGCCAAGCTGGGTACCATCGTGAGCGACGAGTCAACGGCCGTGGGGCTGCCGACGCCCAAGCTCGTTGTGGAGCCCGGACGCGCCATCGCCGGACCGGGCACCATCACGTTGTATGAGGTCGGCACCGTTAAGGACGTCGATGTCAGCGCCACAGCGCATCGACGTTACGTCAGTGTCGACGGCGGCATGAGCGACAACATCCGCACCGCGCTCTACGGCGCGCAGTATGACGTCCGGCTGGTGTCTCGAGTCAGCGACGCCCCGCCGGTACCGGCCCGTCTGGTCGGAAAGCACTGCGAAAGTGGCGATATCATCGTGCGGGACACCTGGGTGCCCGACGATATTCGGCCCGGCGATCTGGTTGCGGTTGCCGCCACCGGCGCTTACTGCTATTCGCTGTCGAGTCGTTACAACATGGTCGGCCGTCCCGCTGTGGTAGCGGTGCACGCGGGCAACGCTCGCCTGGTCCTGCGTCGGGAGACGGTCGACGATTTGCTGAGTTTGGAAGTGAGGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine (Probable). Is essential for the viability of M.tuberculosis in the host (PubMed:12637582). {ECO:0000255|HAMAP-Rule:MF_02120, ECO:0000269|PubMed:12637582, ECO:0000305}.

Family

Orn/Lys/Arg decarboxylase class-II family, LysA subfamily

GO

lysine biosynthetic process via diaminopimelate GO:0009089 biological_process
cell wall GO:0005618 cellular_component
pyridoxal phosphate binding GO:0030170 molecular_function
diaminopimelate decarboxylase activity GO:0008836 molecular_function

InterPro

UniProt: P9WIU7
GenBank: Rv1293
EnsemblBacteria: Rv1293
Mycobrowser: Rv1293

Summary

Name: Diaminopimelate decarboxylase (DAP decarboxylase) (DAPDC) (EC 4.1.1.20)
Family: Orn/Lys/Arg decarboxylase class-II family, LysA subfamily
Protein Sequence: MNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQEYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYAAKAFLCSEVARWISEEGLCLDVCTGGELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMTEIERLDAIAGEAGIVQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGLHSHIGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPIAELAAKLGTIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRRYVSVDGGMSDNIRTALYGAQYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPDDIRPGDLVAVAATGAYCYSLSSRYNMVGRPAVVAVHAGNARLVLRRETVDDLLSLEVR
Mass: 47,458 Da
Length: 447 Aa

Rv1293 doesn't seem to be a targeted by any drug.

Rv1293 doesn't seem to be involved in any pathway.

The three-dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation.: J Struct Funct Genomics. 2009 Sep;10(3):209-17. doi: 10.1007/s10969-009-9065-z. Epub 2009 Jun 19.
Crystal structure of Mycobacterium tuberculosis diaminopimelate decarboxylase, an essential enzyme in bacterial lysine biosynthesis.: J Biol Chem. 2003 May 16;278(20):18588-96. doi: 10.1074/jbc.M301549200. Epub 2003 Mar 10.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of LysA (Rv1293) from Mycobacterium tuberculosis.: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Aug 1;61(Pt 8):782-4. doi: 10.1107/S1744309105022839. Epub 2005 Jul 30.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Cloning of the lysA gene from Mycobacterium tuberculosis.: Gene. 1993 Feb 14;124(1):105-9.