alkA
- Type
- protein_coding
- Name
- alkA
- Locus Name
-
Rv1317c
- Product
-
Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase)
- Functional Category
-
Information pathways
- Location
- 1477628..1479118 (- strand)
- Gene Length
- 1490 bp
- Nucleotides
-
GTGCACGACGACTTCGAACGCTGCTACCGGGCGATCCAGTCCAAAGACGCCCGGTTCGACGGCTGGTTCGTCGTCGCGGTTTTGACCACCGGTGTCTACTGCCGGCCGAGTTGCCCCGTCCGGCCACCGTTCGCGCGCAATGTCCGGTTCCTGCCGACTGCGGCGGCCGCTCAGGGGGAGGGATTCCGGGCCTGCAAACGGTGCCGCCCCGACGCCTCGCCTGGGTCTCCGGAATGGAATGTGCGTAGTGACGTCGTGGCGCGGGCGATGCGGCTGATTGCCGACGGAACGGTGGACCGCGACGGTGTCAGCGGCCTCGCGGCCCAGCTCGGTTACACCATTCGCCAGCTGGAGCGGCTGTTGCAGGCCGTGGTCGGCGCCGGTCCGCTCGCGTTGGCCCGCGCCCAACGCATGCAGACCGCCCGGGTGCTGATCGAGACCACGAACCTGCCGTTCGGCGATGTCGCATTCGCCGCCGGGTTTTCCAGCATCCGTCAGTTCAACGACACCGTTCGCCTGGCGTGCGACGGCACACCGACGGCATTGCGTGCGCGCGCGGCCGCCCGATTCGAGTCTGCCACCGCATCAGCGGGCACGGTGTCGCTGCGGCTACCCGTCCGTGCACCATTCGCCTTCGAGGGTGTTTTCGGCCATCTGGCCGCCACCGCGGTGCCGGGTTGCGAAGAGGTCCGCGATGGTGCGTACCGACGCACGCTACGGCTCCCATGGGGCAACGGCATCGTCAGCCTGACGCCGGCACCCGATCATGTGCGCTGCCTGCTTGTGCTCGATGATTTCCGCGACCTGATGACGGCCACTGCACGTTGCCGACGGCTGCTGGACCTCGACGCCGATCCCGAAGCGATCGTCGAGGCGCTGGGCGCCGATCCGGATCTGCGCGCAGTGGTGGGCAAGGCACCCGGGCAACGCATTCCCCGCACAGTCGACGAGGCAGAATTCGCCGTGCGGGCGGTCCTCGCCCAACAGGTATCGACGAAGGCCGCAAGCACTCACGCGGGCCGACTGGTCGCCGCCTACGGACGGCCGGTCCACGATCGCCACGGCGCTTTGACCCACACCTTCCCGTCGATCGAGCAGCTCGCTGAGATCGATCCCGGCCATCTGGCCGTCCCCAAGGCGCGTCAAAGGACCATAAACGCGCTCGTCGCCAGCCTTGCCGACAAAAGTCTGGTCCTGGACGCCGGATGTGACTGGCAACGCGCCCGCGGGCAGTTGCTAGCGCTGCCCGGAGTGGGCCCCTGGACCGCGGAGGTCATCGCCATGCGCGGCCTCGGTGACCCGGACGCCTTTCCGGCCAGTGATCTCGGCCTGCGGCTGGCCGCCAAAAAGCTGGGCCTGCCTGCACAACGACGAGCCCTGACGGTGCACAGCGCTCGCTGGCGCCCCTGGCGCTCCTATGCCACCCAGCACCTGTGGACCACCCTGGAACATCCGGTAAACCAATGGCCACCGCAGGAGAAGATCGCATG
- Drug Resistance
- Mutations
- Function
- Is involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs the Sp diastereomer of DNA methylphosphotriester lesions by a direct and irreversible transfer of the methyl group to one of its own cysteine residues. Also catalyzes the hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions (By similarity). {ECO:0000250}.; FUNCTION: The methylation of Alka by methylphosphotriesters in DNA leads to its activation as a transcriptional regulator that activates the transcription of its own gene and other alkylation resistance genes. {ECO:0000250}.
- Family
-
Alkylbase DNA glycosidase AlkA family
- GO
-
- alkylated DNA binding GO:0032131 molecular_function
- DNA-3-methylguanine glycosylase activity GO:0052822 molecular_function
- DNA-7-methyladenine glycosylase activity GO:0052821 molecular_function
- DNA-binding transcription factor activity GO:0003700 molecular_function
- protein-DNA complex GO:0032993 cellular_component
- DNA-3-methyladenine glycosylase activity GO:0008725 molecular_function
- cell wall GO:0005618 cellular_component
- base-excision repair, AP site formation GO:0006285 biological_process
- DNA dealkylation involved in DNA repair GO:0006307 biological_process
- plasma membrane GO:0005886 cellular_component
- cytoplasm GO:0005737 cellular_component
- response to nitrosative stress GO:0051409 biological_process
- methyltransferase activity GO:0008168 molecular_function
- zinc ion binding GO:0008270 molecular_function
- DNA-7-methylguanine glycosylase activity GO:0043916 molecular_function
- sequence-specific DNA binding GO:0043565 molecular_function
- InterPro
Summary
- Name
- Probable bifunctional transcriptional activator/DNA repair enzyme AlkA (Regulatory protein AlkA) [Includes: Methylphosphotriester-DNA--protein-cysteine S-methyltransferase (EC 2.1.1.n11) (Methylphosphotriester-DNA methyltransferase); DNA-3-methyladenine glycosylase (EC 3.2.2.21) (DNA-3-methyladenine glycosidase)]
- Family
- Alkylbase DNA glycosidase AlkA family
- Protein Sequence
-
MHDDFERCYRAIQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGEGFRACKRCRPDASPGSPEWNVRSDVVARAMRLIADGTVDRDGVSGLAAQLGYTIRQLERLLQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAGFSSIRQFNDTVRLACDGTPTALRARAAARFESATASAGTVSLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLRLPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLRAVVGKAPGQRIPRTVDEAEFAVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHTFPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLDAGCDWQRARGQLLALPGVGPWTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWTTLEHPVNQWPPQEKIA
- Mass
- 53,743 Da
- Length
- 496 Aa
Rv1317c doesn't seem to be a targeted by any drug.
-
-
Base excision repair mtu03410
mtu03410Genetic Information Processing; Replication and repair
-
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.