Gene - combatTB X

irtA

Type: protein_coding
Name: irtA
Locus Name: Rv1348
Product: Iron-regulated transporter IrtA
Functional Category: Cell wall and cell processes
Location: 1513047..1515626 (+ strand)
Gene Length: 2579 bp
Nucleotides: TGGCACGCGGGTTGCAGGGTGTGATGTTGCGCAGTTTCGGCGCGCGCGACCACACCGCAACGGTGATCGAAACCATTTCGATTGCACCGCATTTCGTGCGGGTCCGGATGGTTTCGCCGACGCTCTTCCAGGATGCGGAGGCTGAGCCCGCCGCATGGCTGCGGTTCTGGTTCCCCGACCCGAACGGGTCCAACACCGAGTTCCAGCGCGCCTATACGATCTCCGAAGCTGACCCCGCCGCGGGCCGCTTCGCGGTCGACGTTGTATTGCATGACCCGGCGGGTCCGGCCTCGTCGTGGGCGCGCACCGTCAAACCTGGCGCAACCATAGCGGTCATGTCGCTGATGGGCTCATCGCGGTTCGACGTGCCCGAGGAGCAGCCCGCCGGGTATCTGCTAATCGGCGACTCGGCGTCGATTCCGGGGATGAACGGGATCATCGAAACGGTCCCGAACGACGTCCCGATCGAGATGTACCTTGAACAACACGACGACAACGACACGTTGATCCCGCTCGCAAAGCATCCCCGGCTGCGGGTGCGCTGGGTTATGCGCCGCGACGAGAAATCGCTGGCCGAGGCGATCGAGAACCGCGACTGGTCGGACTGGTATGCGTGGGCGACGCCAGAGGCTGCCGCGCTGAAATGCGTCCGGGTGCGGCTGCGCGACGAGTTCGGGTTCCCTAAGTCCGAGATCCACGCTCAGGCTTACTGGAACGCCGGGCGTGCCATGGGCACCCACCGAGCAACCGAACCGGCGGCCACCGAACCTGAGGTGGGCGCAGCCCCGCAGCCAGAATCGGCGGTGCCTGCCCCGGCGCGTGGCAGCTGGCGCGCTCAGGCTGCCAGCCGGCTGCTGGCGCCGCTAAAGCTGCCGCTGGTGCTCTCGGGTGTGCTTGCGGCTCTGGTCACGCTGGCGCAGTTGGCGCCGTTCGTGCTGTTGGTCGAGCTGTCAAGGCTGCTGGTCTCCGGCGCCGGCGCGCACCGGTTGTTCACGGTCGGGTTCGCCGCGGTGGGGTTGCTGGGGACCGGGGCCTTGCTGGCAGCCGCCCTCACGCTGTGGCTGCACGTGATCGATGCCCGCTTCGCCAGGGCGTTGCGCTTGCGGCTGCTGAGCAAGCTGTCCCGGTTGCCGCTGGGCTGGTTCACCAGCCGCGGGTCCGGATCGATCAAAAAATTGGTCACCGACGACACGCTGGCGTTGCACTACTTGGTCACCCATGCCGTTCCGGACGCGGTCGCCGCGGTTGTCGCCCCGGTGGGGGTGCTGGTCTATCTGTTCGTCGTGGACTGGCGAGTGGCGCTGGTCTTGTTCGGGCCGGTTCTGGTCTACCTGACCATCACGTCATCGCTCACGATCCAATCCGGGCCCCGCATTGTTCAAGCGCAGCGGTGGGCAGAGAAGATGAACGGCGAAGCGGGTAGTTACCTCGAGGGTCAGCCGGTGATTCGCGTCTTCGGCGCCGCGTCATCGAGCTTCCGTCGCCGGTTGGACGAGTACATCGGATTCCTGGTCGCCTGGCAGCGGCCGCTGGCCGGCAAGAAAACCCTGATGGATCTGGCCACTCGCCCAGCAACGTTCCTGTGGCTCATCGCCGCTACCGGCACCTTGTTGGTAGCCACGCATCGAATGGATCCGGTGAATTTGTTGCCGTTCATGTTCTTGGGTACCACGTTCGGTGCCCGCCTGCTCGGGATCGCCTACGGGCTCGGCGGCCTACGCACGGGACTTCTGGCGGCCCGGCACCTGCAAGTCACACTCGACGAAACCGAACTCGCCGTGCGGGAACATCCGCGCGAACCGCTCGACGGCGAGGCGCCAGCAACTGTGGTGTTCGACCACGTCACCTTCGGGTACCGCCCTGGAGTGCCGGTGATCCAGGATGTATCGCTTACGCTGCGGCCGGGCACGGTCACCGCGCTCGTCGGCCCGTCCGGCTCCGGCAAGTCGACACTGGCCACCCTGCTGGCTCGATTCCACGATGTCGAGCGAGGTGCGATACGCGTTGGTGGACAGGATATTCGATCACTGGCCGCGGACGAGCTGTACACGCGAGTCGGCTTTGTGCTACAGGAAGCCCAGCTTGTGCATGGCACCGCCGCCGAAAACATCGCGCTGGCGGTACCGGATGCCCCCGCCGAACAGGTCCAGGTCGCGGCCCGCGAAGCGCAAATCCACGACCGGGTGCTTCGGCTGCCGGACGGCTACGATACCGTGCTCGGAGCCAACAGTGGTCTTTCGGGCGGGGAGCGACAGCGGCTCACCATTGCCCGTGCCATCCTCGGCGACACTCCGGTCCTCATCCTCGACGAGGCCACCGCGTTTGCCGATCCGGAATCGGAATACCTTGTGCAACAGGCGCTTAACCGGCTGACCCGGGACCGCACCGTGCTGGTAATCGCCCATCGACTGCATACCATCACCCGGGCCGACCAGATCGTCGTGCTCGATCATGGTCGGATCGTCGAACGCGGCACCCACGAGGAGTTGCTTGCCGCGGGCGGACGCTACTGCCGGCTGTGGGACACCGGCCAGGGCAGCCGGGTGGCGGTCGCCGCAGCGCAGGACGGCACCCGATGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Part of the ABC transporter complex IrtAB involved in iron import. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation. Required for replication in human macrophages and in mouse lungs. {ECO:0000269|PubMed:16385031, ECO:0000269|PubMed:19948799}.

Family

ABC transporter superfamily, Siderophore-Fe(3+) uptake transporter (SIUT) (TC 3.A.1.21) family

GO

FAD binding GO:0071949 molecular_function
cellular response to iron ion starvation GO:0010106 biological_process
pathogenesis GO:0009405 biological_process
siderophore transport GO:0015891 biological_process
iron ion homeostasis GO:0055072 biological_process
siderophore transmembrane transporter activity GO:0015343 molecular_function
ATP binding GO:0005524 molecular_function
integral component of plasma membrane GO:0005887 cellular_component
ATPase-coupled transmembrane transporter activity GO:0042626 molecular_function
plasma membrane GO:0005886 cellular_component
acquisition by symbiont of nutrients from host via siderophores GO:0052099 biological_process
siderophore-dependent iron import into cell GO:0033214 biological_process
response to host iron concentration GO:0075139 biological_process
oxidoreductase activity GO:0016491 molecular_function
iron ion transport GO:0006826 biological_process

InterPro

UniProt: P9WQJ9
GenBank: Rv1348
EnsemblBacteria: Rv1348
Mycobrowser: Rv1348

Summary

Name: Iron import ATP-binding/permease protein IrtA (EC 3.6.3.-) (Iron-regulated transporter A)
Family: ABC transporter superfamily, Siderophore-Fe(3+) uptake transporter (SIUT) (TC 3.A.1.21) family
Protein Sequence: MARGLQGVMLRSFGARDHTATVIETISIAPHFVRVRMVSPTLFQDAEAEPAAWLRFWFPDPNGSNTEFQRAYTISEADPAAGRFAVDVVLHDPAGPASSWARTVKPGATIAVMSLMGSSRFDVPEEQPAGYLLIGDSASIPGMNGIIETVPNDVPIEMYLEQHDDNDTLIPLAKHPRLRVRWVMRRDEKSLAEAIENRDWSDWYAWATPEAAALKCVRVRLRDEFGFPKSEIHAQAYWNAGRAMGTHRATEPAATEPEVGAAPQPESAVPAPARGSWRAQAASRLLAPLKLPLVLSGVLAALVTLAQLAPFVLLVELSRLLVSGAGAHRLFTVGFAAVGLLGTGALLAAALTLWLHVIDARFARALRLRLLSKLSRLPLGWFTSRGSGSIKKLVTDDTLALHYLVTHAVPDAVAAVVAPVGVLVYLFVVDWRVALVLFGPVLVYLTITSSLTIQSGPRIVQAQRWAEKMNGEAGSYLEGQPVIRVFGAASSSFRRRLDEYIGFLVAWQRPLAGKKTLMDLATRPATFLWLIAATGTLLVATHRMDPVNLLPFMFLGTTFGARLLGIAYGLGGLRTGLLAARHLQVTLDETELAVREHPREPLDGEAPATVVFDHVTFGYRPGVPVIQDVSLTLRPGTVTALVGPSGSGKSTLATLLARFHDVERGAIRVGGQDIRSLAADELYTRVGFVLQEAQLVHGTAAENIALAVPDAPAEQVQVAAREAQIHDRVLRLPDGYDTVLGANSGLSGGERQRLTIARAILGDTPVLILDEATAFADPESEYLVQQALNRLTRDRTVLVIAHRLHTITRADQIVVLDHGRIVERGTHEELLAAGGRYCRLWDTGQGSRVAVAAAQDGTR
Mass: 92,966 Da
Length: 859 Aa

Rv1348 doesn't seem to be a targeted by any drug.

Mtb iron assimilation by chelation R-HSA-1222449
Homo sapiens
R-HSA-1222449

ABC transporters mtu02010
mtu02010
Environmental Information Processing; Membrane transport

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
The Mycobacterium tuberculosis high-affinity iron importer, IrtA, contains an FAD-binding domain.: J Bacteriol. 2010 Feb;192(3):861-9. doi: 10.1128/JB.00223-09. Epub 2009 Nov 30.
Mechanistic insights into a novel exporter-importer system of Mycobacterium tuberculosis unravel its role in trafficking of iron.: PLoS One. 2008 May 7;3(5):e2087. doi: 10.1371/journal.pone.0002087.
Identification of an ABC transporter required for iron acquisition and virulence in Mycobacterium tuberculosis.: J Bacteriol. 2006 Jan;188(2):424-30. doi: 10.1128/JB.188.2.424-430.2006.
ideR, An essential gene in mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response.: Infect Immun. 2002 Jul;70(7):3371-81. doi: 10.1128/iai.70.7.3371-3381.2002.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.