Gene - combatTB X

priA

Type: protein_coding
Name: priA
Locus Name: Rv1402
Product: Putative primosomal protein N' PriA (replication factor Y)
Functional Category: Information pathways
Location: 1577613..1579580 (+ strand)
Gene Length: 1967 bp
Nucleotides: TGTTGTCGGTGCCGCACCTGGACCGCGACTTCGACTACTTGGTGCCCGCCGAACACTCCGACGATGCCCAGCCGGGGGTGCGGGTACGGGTGCGGTTTCACGGTCGGCTGGTCGACGGGTTTGTCCTAGAGCGCCGCAGCGACAGCGATCACCACGGCAAGCTGGGCTGGCTGGATCGTGTGGTGTCGCCCGAACCGGTGCTCACCACGGAGATCCGCCGGTTGGTCGATGCGGTGGCGGCGCGCTACGCCGGGACCCGCCAGGACGTATTGCGGCTCGCAGTGCCCGCCCGGCACGCACGGGTGGAGCGGGAAATCACCACGGCCCCGGGTCGGCCGGTGGTAGCGCCGGTCGACCCGTCGGGTTGGGCGGCCTACGGTCGCGGTCGGCAATTCCTGGCCGCGCTGGCCGACTCGCGCGCTGCGCGGGCCGTTTGGCAGGCGCTACCGGGCGAGCTGTGGGCGGACCGATTCGCCGAGGCTGCCGCGCAGACCGTACGTGCCGGGCGCACGGTACTGGCGATCGTGCCCGATCAGCGGGATCTGGACACCCTGTGGCAGGCCGCGACGGCCCTCGTCGATGAGCACAGTGTGGTAGCACTGTCGGCCGGCCTGGGCCCGGAGGCACGCTATCGGCGCTGGCTGGCCGCGTTGCGGGGCAGCGCGCGGCTGGTGATTGGCACCCGCAGCGCGGTGTTCGCGCCGTTGAGCGAGCTGGGCCTGGTCATGGTCTGGGCCGACGCCGACGACTCCCTGGCTGAGCCGCGGGCACCCTATCCGCACGCCCGTGAGGTGGCGATGCTGCGGGCGCATCAGGCGCGGTGCGCAGCGCTGATCGGCGGCTACGCCCGCACGGCCGAGGCCCACGCGCTGGTGCGTAGCGGCTGGGCGCACGACGTGGTTGCACCCCGGCCGGAGGTGCGTGCACGCTCTCCTCGCGTGGTTGCCCTCGACGACAGCGGATACGACGACGCGCGAGACCCGGCCGCCCGCACCGCACGGCTACCGTCCATCGCGCTGCGCGCCGCGCGCTCAGCGCTGCAGTCCGGGGCGCCGGTGCTGGTGCAGGTGCCGCGGCGCGGGTACATCCCCTCGCTGGCCTGCGGGCGCTGCCGGGCGATCGCTCGTTGCCGGTCGTGCACGGGTCCGCTATCGCTGCAAGGCGCCGGCTCGCCCGGTGCGGTATGTCGCTGGTGTGGACGGGTGGACCCGACACTGCGATGCGTGCGCTGTGGGTCGGACGTGGTGCGTGCCGTGGTGGTGGGGGCCCGGCGCACTGCCGAAGAGCTCGGCCGGGCATTCCCGGGTACGGCGGTGATTACGTCGGCCGGCGACACCCTGGTGCCCCAGCTCGACGCCGGCCCAGCCCTGGTGGTCGCCACTCCAGGAGCCGAACCCCGGGCGCCCGGCGGGTATGGGGCGGCGCTGCTGCTGGATAGCTGGGCGCTGCTGGGCCGTCAAGACTTGCGCGCGGCCGAGGACGCGCTGTGGCGCTGGATGACGGCGGCCGCCCTGGTTCGGCCGCGCGGGGCGGGCGGTGTGGTGACCGTGGTCGCCGAATCGTCCATTCCGACAGTGCAATCGCTGATCCGGTGGGATCCGGTCGGTCACGCGGAGGCCGAACTGGCAGCCCGAACCGAAGTCGGCCTGCCGCCAAGTGTGCACATCGCTGCTCTTGACGGCCCTGCCGGCACCGTGACGGCATTGCTGGAGGCGGCTCGGCTGCCCGACCCGGATCGCCTCCAAGCCGATCTGCTGGGCCCGGTGGACCTGCCACCCGGCGTCCGTCGCCCGGCGGGCATCCCCGCCGATGCGCCGGTCATCAGGATGTTGCTGCGGGTGTGCCGCGAGCAGGGCCTGGAGTTGGCGGCGAGTCTGCGGCGCGGCATCGGTGTGCTCAGTGCGCGGCAAACCCGGCAAACCCGTAGCCTGGTTCGGGTACAGATTGACCCGCTGCATATCGGGTAA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-Rule:MF_00983}.

Family

Helicase family, PriA subfamily

GO

primosome complex GO:1990077 cellular_component
ATP binding GO:0005524 molecular_function
DNA binding GO:0003677 molecular_function
DNA replication initiation GO:0006270 biological_process
DNA replication, synthesis of RNA primer GO:0006269 biological_process
DNA unwinding involved in DNA replication GO:0006268 biological_process
DNA recombination GO:0006310 biological_process
double-strand break repair GO:0006302 biological_process
plasma membrane GO:0005886 cellular_component
3'-5' DNA helicase activity GO:0043138 molecular_function
zinc ion binding GO:0008270 molecular_function

InterPro

UniProt: P9WMQ9
GenBank: Rv1402
EnsemblBacteria: Rv1402
Mycobrowser: Rv1402

Summary

Name: Probable primosomal protein N' (EC 3.6.4.-) (ATP-dependent helicase PriA)
Family: Helicase family, PriA subfamily
Protein Sequence: MLSVPHLDRDFDYLVPAEHSDDAQPGVRVRVRFHGRLVDGFVLERRSDSDHHGKLGWLDRVVSPEPVLTTEIRRLVDAVAARYAGTRQDVLRLAVPARHARVEREITTAPGRPVVAPVDPSGWAAYGRGRQFLAALADSRAARAVWQALPGELWADRFAEAAAQTVRAGRTVLAIVPDQRDLDTLWQAATALVDEHSVVALSAGLGPEARYRRWLAALRGSARLVIGTRSAVFAPLSELGLVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAHALVRSGWAHDVVAPRPEVRARSPRVVALDDSGYDDARDPAARTARLPSIALRAARSALQSGAPVLVQVPRRGYIPSLACGRCRAIARCRSCTGPLSLQGAGSPGAVCRWCGRVDPTLRCVRCGSDVVRAVVVGARRTAEELGRAFPGTAVITSAGDTLVPQLDAGPALVVATPGAEPRAPGGYGAALLLDSWALLGRQDLRAAEDALWRWMTAAALVRPRGAGGVVTVVAESSIPTVQSLIRWDPVGHAEAELAARTEVGLPPSVHIAALDGPAGTVTALLEAARLPDPDRLQADLLGPVDLPPGVRRPAGIPADAPVIRMLLRVCREQGLELAASLRRGIGVLSARQTRQTRSLVRVQIDPLHIG
Mass: 69,839 Da
Length: 655 Aa

Rv1402 doesn't seem to be a targeted by any drug.

Homologous recombination mtu03440
mtu03440
Genetic Information Processing; Replication and repair

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.