Gene - combatTB X

gap

Type: protein_coding
Name: gap
Locus Name: Rv1436
Product: Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH)
Functional Category: Intermediary metabolism and respiration
Location: 1613307..1614326 (+ strand)
Gene Length: 1019 bp
Nucleotides: TGACGGTCCGAGTAGGCATCAACGGGTTTGGTCGAATCGGACGCAACTTCTACCGGGCCTTACTGGCCCAACAGGAGCAGGGCACCGCCGACGTGGAGGTGGTCGCCGCCAACGACATCACCGACAACAGCACGCTGGCGCATCTGCTCAAATTCGACTCGATTCTGGGCCGGCTGCCTTGCGATGTCGGCCTCGAAGGCGACGACACCATCGTCGTCGGCCGCGCGAAAATCAAGGCGCTCGCGGTCCGGGAGGGGCCGGCGGCATTGCCATGGGGAGACCTCGGCGTCGACGTCGTCGTCGAATCCACCGGCCTGTTCACCAATGCGGCCAAAGCCAAAGGCCACCTGGACGCCGGCGCCAAGAAGGTGATCATCTCTGCGCCCGCCACCGACGAGGACATCACCATCGTCCTGGGAGTTAACGACGACAAGTATGACGGCAGCCAGAACATCATCTCCAATGCGTCGTGCACCACGAACTGCCTTGCGCCGCTGGCCAAAGTGCTCGACGATGAGTTCGGCATCGTCAAGGGCCTGATGACCACCATCCACGCCTACACTCAGGATCAGAACCTGCAGGACGGGCCGCACAAGGACCTGCGTCGCGCCCGCGCCGCCGCGCTGAACATCGTGCCGACCTCCACCGGCGCGGCCAAGGCCATCGGCCTGGTGATGCCGCAGCTAAAGGGCAAGCTCGACGGTTATGCGCTGCGGGTGCCGATCCCCACCGGCTCGGTCACCGACCTTACGGTCGACTTATCCACACGGGCCAGTGTCGATGAGATCAACGCGGCGTTCAAAGCCGCGGCCGAAGGCAGGCTCAAGGGCATTCTGAAGTACTACGACGCGCCGATCGTCTCGAGCGACATCGTCACCGACCCGCACAGTTCGATTTTCGACTCTGGGTTGACCAAAGTCATCGACGACCAGGCCAAGGTGGTGTCGTGGTACGACAACGAGTGGGGCTACTCCAACCGCCTGGTTGATCTGGTCACGCTGGTCGGCAAGTCGCTCTAG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG. {ECO:0000269|PubMed:24161676}.

Family

Glyceraldehyde-3-phosphate dehydrogenase family

GO

glycolytic process GO:0006096 biological_process
glucose metabolic process GO:0006006 biological_process
cell wall GO:0005618 cellular_component
extracellular region GO:0005576 cellular_component
cytosol GO:0005829 cellular_component
plasma membrane GO:0005886 cellular_component
NADP binding GO:0050661 molecular_function
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 molecular_function
NAD binding GO:0051287 molecular_function

InterPro

UniProt: P9WN83
GenBank: Rv1436
EnsemblBacteria: Rv1436
Mycobrowser: Rv1436

Summary

Name: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (NAD-dependent glyceraldehyde-3-phosphate dehydrogenase)
Family: Glyceraldehyde-3-phosphate dehydrogenase family
Protein Sequence: MTVRVGINGFGRIGRNFYRALLAQQEQGTADVEVVAANDITDNSTLAHLLKFDSILGRLPCDVGLEGDDTIVVGRAKIKALAVREGPAALPWGDLGVDVVVESTGLFTNAAKAKGHLDAGAKKVIISAPATDEDITIVLGVNDDKYDGSQNIISNASCTTNCLAPLAKVLDDEFGIVKGLMTTIHAYTQDQNLQDGPHKDLRRARAAALNIVPTSTGAAKAIGLVMPQLKGKLDGYALRVPIPTGSVTDLTVDLSTRASVDEINAAFKAAAEGRLKGILKYYDAPIVSSDIVTDPHSSIFDSGLTKVIDDQAKVVSWYDNEWGYSNRLVDLVTLVGKSL
Mass: 35,956 Da
Length: 339 Aa

Rv1436 doesn't seem to be a targeted by any drug.

Glycolysis / Gluconeogenesis mtu00010
mtu00010
Metabolism; Carbohydrate metabolism

Kinetic and mechanistic characterization of the glyceraldehyde 3-phosphate dehydrogenase from Mycobacterium tuberculosis.: Arch Biochem Biophys. 2013 Dec;540(1-2):53-61. doi: 10.1016/j.abb.2013.10.007. Epub 2013 Oct 23.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.