Gene - combatTB X

acn

Type: protein_coding
Name: acn
Locus Name: Rv1475c
Product: Probable iron-regulated aconitate hydratase Acn (citrate hydro-lyase) (aconitase)
Functional Category: Intermediary metabolism and respiration
Location: 1663215..1666046 (- strand)
Gene Length: 2831 bp
Nucleotides: GTGACTAGCAAATCTGTGAACTCATTCGGAGCCCACGACACCCTGAAGGTCGGCGAAAAGAGTTACCAGATCTATCGTCTCGACGCCGTCCCCAATACCGCGAAACTCCCCTACAGCCTCAAAGTGCTCGCCGAGAACCTGTTGCGCAACGAGGACGGCAGCAACATCACCAAGGACCACATCGAGGCCATCGCCAACTGGGACCCTAAGGCCGAGCCCAGCATCGAGATCCAGTACACGCCCGCCCGGGTGGTGATGCAGGACTTCACCGGCGTACCGTGCATCGTCGACTTGGCCACCATGCGCGAGGCGATCGCCGATCTGGGCGGCAACCCGGACAAGGTCAACCCGCTGGCGCCCGCAGACTTGGTGATCGACCACTCGGTGATCGCCGATTTGTTCGGCCGCGCCGACGCATTCGAGCGCAACGTCGAAATCGAATACCAGCGCAACGGTGAGCGTTACCAATTCCTGCGCTGGGGCCAAGGCGCTTTCGACGACTTCAAAGTGGTGCCGCCGGGCACCGGCATCGTGCACCAGGTCAATATCGAGTACCTGGCCAGCGTGGTGATGACTCGCGACGGAGTGGCCTACCCCGACACCTGCGTGGGCACCGACTCACACACCACCATGGTCAACGGCCTGGGTGTGCTCGGGTGGGGTGTCGGCGGCATCGAGGCGGAGGCCGCGATGCTGGGTCAGCCGGTATCGATGCTGATCCCGCGGGTCGTGGGTTTCAGGTTGACCGGCGAGATCCAGCCGGGAGTCACCGCCACCGACGTGGTGTTGACCGTCACCGAGATGCTGCGCCAGCACGGCGTCGTCGGCAAATTCGTCGAGTTCTACGGCGAGGGCGTGGCCGAGGTGCCGCTGGCCAACCGCGCCACCCTGGGCAACATGAGTCCCGAATTCGGTTCCACCGCAGCGATTTTCCCGATCGACGAAGAAACCATCAAGTATCTGCGGTTTACCGGTCGCACGCCGGAGCAGGTCGCACTGGTCGAGGCCTACGCCAAGGCGCAGGGCATGTGGCACGATCCCAAGCACGAGCCGGAGTTCTCGGAATACCTCGAACTCAACCTATCCGACGTGGTGCCGTCGATCGCCGGACCAAAGCGTCCACAGGACCGAATCGCGTTGGCGCAAGCCAAATCAACATTCCGCGAGCAGATTTACCACTATGTCGGCAATGGTTCCCCGGATTCCCCCCACGACCCGCACTCGAAGCTGGACGAGGTAGTCGAGGAGACATTCCCGGCCAGCGACCCGGGGCAGCTGACGTTCGCCAACGACGACGTCGCCACTGACGAAACCGTGCACTCGGCTGCCGCGCATGCCGATGGCCGGGTGAGCAACCCAGTGCGGGTGAAGTCGGACGAACTCGGCGAATTCGTGCTCGACCACGGCGCGGTGGTGATTGCCGCGATCACGTCCTGCACCAACACCTCCAACCCCGAAGTAATGCTGGGCGCGGCGCTGCTGGCCCGCAACGCCGTCGAAAAGGGACTGACCTCGAAGCCGTGGGTGAAGACCACGATTGCCCCGGGCTCGCAAGTGGTCAACGACTACTACGACAGATCCGGCCTGTGGCCGTATCTGGAGAAGCTCGGCTTCTATCTGGTCGGCTACGGCTGCACCACCTGCATCGGCAACTCCGGGCCGCTGCCCGAGGAAATCTCAAAAGCGGTTAACGACAACGACCTTTCGGTGACCGCGGTACTGTCCGGCAACCGGAACTTCGAGGGCCGTATCAACCCAGACGTGAAGATGAACTACCTGGCATCGCCGCCGCTGGTCATCGCCTACGCGCTGGCCGGGACCATGGACTTCGACTTCCAAACCCAGCCGCTCGGTCAAGACAAAGACGGTAAGAACGTTTTTCTCCGCGATATCTGGCCATCGCAGCAGGATGTCTCCGACACCATCGCCGCGGCGATCAACCAGGAGATGTTCACCCGCAACTACGCCGACGTGTTCAAGGGCGACGACCGCTGGCGCAACCTGCCAACCCCGAGCGGCAACACCTTTGAGTGGGACCCGAATTCGACCTACGTGCGCAAGCCGCCGTATTTCGAGGGGATGACAGCCAAACCCGAACCGGTCGGCAACATCAGCGGTGCCCGGGTGCTGGCGCTGCTCGGTGATTCGGTGACCACCGACCACATCTCCCCCGCCGGCGCCATCAAGCCCGGAACTCCCGCGGCGCGCTACCTCGACGAACACGGTGTCGACCGCAAGGACTACAACTCCTTCGGTTCTCGCCGCGGCAACCACGAGGTGATGATTCGTGGCACCTTCGCCAACATCCGGCTGCGTAACCAACTGCTAGACGACGTGTCCGGCGGTTATACCCGCGACTTCACCCAGCCGGGCGGTCCGCAGGCGTTCATCTACGACGCCGCGCAGAACTATGCGGCGCAACACATTCCGCTGGTTGTGTTCGGCGGCAAAGAGTACGGGTCGGGTTCGTCACGGGACTGGGCGGCCAAAGGCACATTGCTACTGGGCGTGCGGGCGGTGATCGCCGAGTCATTCGAGCGGATCCACCGGTCCAACCTGATCGGCATGGGCGTGATCCCGCTGCAGTTCCCCGAAGGAAAGTCAGCGTCGTCGTTGGGACTCGACGGTACCGAGGTCTTCGACATCACCGGTATCGACGTGCTTAACGACGGCAAGACACCCAAGACGGTGTGCGTCCAGGCCACCAAGGGCGACGGCGCCACGATCGAGTTCGACGCCGTGGTGCGCATCGACACCCCCGGTGAGGCGGACTACTACCGCAACGGCGGCATCCTGCAGTACGTGCTGCGCAACATACTGAAGTCAGGCTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein which binds to selected IRE-like sequences present within the UTRs (untranslated regions) of 3' trxC and 5' IdeR mRNA (PubMed:17384188). Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate (By similarity). {ECO:0000250|UniProtKB:Q8ZP52, ECO:0000269|PubMed:17384188}.

Family

Aconitase/IPM isomerase family

GO

metal ion binding GO:0046872 molecular_function
propionate metabolic process, methylcitrate cycle GO:0019679 biological_process
response to iron ion GO:0010039 biological_process
mRNA binding GO:0003729 molecular_function
mRNA 3'-UTR binding GO:0003730 molecular_function
aconitate hydratase activity GO:0003994 molecular_function
iron-responsive element binding GO:0030350 molecular_function
4 iron, 4 sulfur cluster binding GO:0051539 molecular_function
plasma membrane GO:0005886 cellular_component
2-methylisocitrate dehydratase activity GO:0047456 molecular_function
cytosol GO:0005829 cellular_component
extracellular region GO:0005576 cellular_component
cell wall GO:0005618 cellular_component
citrate metabolic process GO:0006101 biological_process
tricarboxylic acid cycle GO:0006099 biological_process

InterPro

UniProt: O53166
GenBank: Rv1475c
EnsemblBacteria: Rv1475c
Mycobrowser: Rv1475c

Summary

Name: Aconitate hydratase A (ACN) (Aconitase) (EC 4.2.1.3) ((2R,3S)-2-methylisocitrate dehydratase) ((2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase) (Iron-responsive protein-like) (IRP-like) (Probable 2-methyl-cis-aconitate hydratase) (EC 4.2.1.99) (RNA-binding protein)
Family: Aconitase/IPM isomerase family
Protein Sequence: MTSKSVNSFGAHDTLKVGEKSYQIYRLDAVPNTAKLPYSLKVLAENLLRNEDGSNITKDHIEAIANWDPKAEPSIEIQYTPARVVMQDFTGVPCIVDLATMREAIADLGGNPDKVNPLAPADLVIDHSVIADLFGRADAFERNVEIEYQRNGERYQFLRWGQGAFDDFKVVPPGTGIVHQVNIEYLASVVMTRDGVAYPDTCVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPRVVGFRLTGEIQPGVTATDVVLTVTEMLRQHGVVGKFVEFYGEGVAEVPLANRATLGNMSPEFGSTAAIFPIDEETIKYLRFTGRTPEQVALVEAYAKAQGMWHDPKHEPEFSEYLELNLSDVVPSIAGPKRPQDRIALAQAKSTFREQIYHYVGNGSPDSPHDPHSKLDEVVEETFPASDPGQLTFANDDVATDETVHSAAAHADGRVSNPVRVKSDELGEFVLDHGAVVIAAITSCTNTSNPEVMLGAALLARNAVEKGLTSKPWVKTTIAPGSQVVNDYYDRSGLWPYLEKLGFYLVGYGCTTCIGNSGPLPEEISKAVNDNDLSVTAVLSGNRNFEGRINPDVKMNYLASPPLVIAYALAGTMDFDFQTQPLGQDKDGKNVFLRDIWPSQQDVSDTIAAAINQEMFTRNYADVFKGDDRWRNLPTPSGNTFEWDPNSTYVRKPPYFEGMTAKPEPVGNISGARVLALLGDSVTTDHISPAGAIKPGTPAARYLDEHGVDRKDYNSFGSRRGNHEVMIRGTFANIRLRNQLLDDVSGGYTRDFTQPGGPQAFIYDAAQNYAAQHIPLVVFGGKEYGSGSSRDWAAKGTLLLGVRAVIAESFERIHRSNLIGMGVIPLQFPEGKSASSLGLDGTEVFDITGIDVLNDGKTPKTVCVQATKGDGATIEFDAVVRIDTPGEADYYRNGGILQYVLRNILKSG
Mass: 102,449 Da
Length: 943 Aa

Rv1475c doesn't seem to be a targeted by any drug.

Glyoxylate and dicarboxylate metabolism mtu00630
mtu00630
PATHWAY_MAP mtu00630 Glyoxylate and dicarboxylate metabolism

Citrate cycle (TCA cycle) mtu00020
mtu00020
Metabolism; Carbohydrate metabolism

Mycobacterium tuberculosis Rv1474c is a TetR-like transcriptional repressor that regulates aconitase, an essential enzyme and RNA-binding protein, in an iron-responsive manner.: Tuberculosis (Edinb). 2017 Mar;103:71-82. doi: 10.1016/j.tube.2017.01.003. Epub 2017 Jan 17.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Iron-dependent RNA-binding activity of Mycobacterium tuberculosis aconitase.: J Bacteriol. 2007 Jun;189(11):4046-52. doi: 10.1128/JB.00026-07. Epub 2007 Mar 23.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.