Gene - combatTB X

fabG1

Type: protein_coding
Name: fabG1
Locus Name: Rv1483
Product: 3-oxoacyl-[acyl-carrier protein] reductase FabG1 (3-ketoacyl-acyl carrier protein reductase) (mycolic acid biosynthesis a protein)
Functional Category: Lipid metabolism
Location: 1673440..1674183 (+ strand)
Gene Length: 743 bp
Nucleotides: TGACTGCCACAGCCACTGAAGGGGCCAAACCCCCATTCGTATCCCGTTCAGTCCTGGTTACCGGAGGAAACCGGGGGATCGGGCTGGCGATCGCACAGCGGCTGGCTGCCGACGGCCACAAGGTGGCCGTCACCCACCGTGGATCCGGAGCGCCAAAGGGGCTGTTTGGCGTCGAATGTGACGTCACCGACAGCGACGCCGTCGATCGCGCCTTCACGGCGGTAGAAGAGCACCAGGGTCCGGTCGAGGTGCTGGTGTCCAACGCCGGCCTATCCGCGGACGCATTCCTCATGCGGATGACCGAGGAAAAGTTCGAGAAGGTCATCAACGCCAACCTCACCGGGGCGTTCCGGGTGGCTCAACGGGCATCGCGCAGCATGCAGCGCAACAAATTCGGTCGAATGATATTCATAGGTTCGGTCTCCGGCAGCTGGGGCATCGGCAACCAGGCCAACTACGCAGCCTCCAAGGCCGGAGTGATTGGCATGGCCCGCTCGATCGCCCGCGAGCTGTCGAAGGCAAACGTGACCGCGAATGTGGTGGCCCCGGGCTACATCGACACCGATATGACCCGCGCGCTGGATGAGCGGATTCAGCAGGGGGCGCTGCAATTTATCCCAGCGAAGCGGGTCGGCACCCCCGCCGAGGTCGCCGGGGTGGTCAGCTTCCTGGCTTCCGAGGATGCGAGCTATATCTCCGGTGCGGTCATCCCGGTCGACGGCGGCATGGGTATGGGCCACTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis. MabA preferentially metabolizes long-chain substrates (C8-C20) and has a poor affinity for the C4 substrate. {ECO:0000269|PubMed:11932442, ECO:0000269|PubMed:19685079, ECO:0000269|PubMed:9802011}.

Family

Short-chain dehydrogenases/reductases (SDR) family

GO

short-chain fatty acid metabolic process GO:0046459 biological_process
mycolic acid biosynthetic process GO:0071768 biological_process
3-oxo-glutaryl-[acp] methyl ester reductase activity GO:0102131 molecular_function
3-oxo-pimeloyl-[acp] methyl ester reductase activity GO:0102132 molecular_function
plasma membrane GO:0005886 cellular_component
NADPH binding GO:0070402 molecular_function
oxidation-reduction process GO:0055114 biological_process
acetoacetyl-CoA reductase activity GO:0018454 molecular_function
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity GO:0004316 molecular_function

InterPro

UniProt: P9WGT3
GenBank: Rv1483
EnsemblBacteria: Rv1483
Mycobrowser: Rv1483

Summary

Name: 3-oxoacyl-[acyl-carrier-protein] reductase FabG1 (EC 1.1.1.100) (3-ketoacyl-acyl carrier protein reductase) (Beta-ketoacyl-ACP reductase) (Beta-ketoacyl-acyl carrier protein reductase)
Family: Short-chain dehydrogenases/reductases (SDR) family
Protein Sequence: MTATATEGAKPPFVSRSVLVTGGNRGIGLAIAQRLAADGHKVAVTHRGSGAPKGLFGVECDVTDSDAVDRAFTAVEEHQGPVEVLVSNAGLSADAFLMRMTEEKFEKVINANLTGAFRVAQRASRSMQRNKFGRMIFIGSVSGSWGIGNQANYAASKAGVIGMARSIARELSKANVTANVVAPGYIDTDMTRALDERIQQGALQFIPAKRVGTPAEVAGVVSFLASEDASYISGAVIPVDGGMGMGH
Mass: 25,697 Da
Length: 247 Aa

Rv1483 doesn't seem to be a targeted by any drug.

Rv1483 doesn't seem to be involved in any pathway.

Lack of dynamics in the MabA active site kills the enzyme activity: practical consequences for drug-design studies.: Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):923-5. doi: 10.1107/S0907444907024158. Epub 2007 Jul 17.
Crystal structure of MabA from Mycobacterium tuberculosis, a reductase involved in long-chain fatty acid biosynthesis.: J Mol Biol. 2002 Jul 5;320(2):249-61. doi: 10.1016/S0022-2836(02)00463-1.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
The essential mycobacterial genes, fabG1 and fabG4, encode 3-oxoacyl-thioester reductases that are functional in yeast mitochondrial fatty acid synthase type 2.: Mol Genet Genomics. 2009 Oct;282(4):407-16. doi: 10.1007/s00438-009-0474-2. Epub 2009 Aug 14.
MabA (FabG1), a Mycobacterium tuberculosis protein involved in the long-chain fatty acid elongation system FAS-II.: Microbiology. 2002 Apr;148(Pt 4):951-60. doi: 10.1099/00221287-148-4-951.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
The mabA gene from the inhA operon of Mycobacterium tuberculosis encodes a 3-ketoacyl reductase that fails to confer isoniazid resistance.: Microbiology. 1998 Oct;144 ( Pt 10):2697-704. doi: 10.1099/00221287-144-10-2697.