Gene - combatTB X

hemZ

Type: protein_coding
Name: hemZ
Locus Name: Rv1485
Product: Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase)
Functional Category: Intermediary metabolism and respiration
Location: 1675017..1676051 (+ strand)
Gene Length: 1034 bp
Nucleotides: TGCAATTTGATGCCGTCCTGCTGCTGTCGTTCGGCGGACCGGAAGGGCCCGAGCAGGTGCGGCCGTTCCTGGAGAACGTTACCCGGGGCCGCGGTGTGCCTGCCGAACGGTTGGACGCGGTGGCCGAGCACTACCTGCATTTCGGTGGGGTATCACCGATCAATGGCATTAATCGCACACTGATCGCGGAGCTGGAGGCGCAGCAAGAACTGCCGGTGTACTTCGGTAACCGCAACTGGGAGCCGTATGTAGAAGATGCCGTTACGGCCATGCGCGACAACGGTGTCCGGCGTGCAGCGGTCTTTGCGACATCTGCGTGGAGCGGTTACTCGAGCTGCACACAGTACGTGGAGGACATCGCGCGGGCCCGCCGCGCGGCCGGGCGCGACGCGCCTGAACTGGTAAAACTGCGGCCCTACTTCGACCATCCGCTGTTCGTCGAGATGTTCGCCGACGCCATCACCGCGGCCGCCGCAACCGTGCGCGGTGATGCCCGGCTGGTGTTCACCGCGCATTCGATCCCGACGGCCGCCGACCGCCGCTGTGGCCCCAACCTCTACAGCCGCCAAGTCGCCTACGCCACAAGGCTGGTCGCGGCCGCTGCCGGATACTGCGACTTTGACCTGGCCTGGCAGTCGAGATCGGGCCCGCCGCAGGTGCCCTGGCTGGAGCCAGACGTTACCGACCAGCTCACCGGTCTGGCTGGGGCCGGCATCAACGCGGTGATCGTGTGTCCCATTGGATTCGTCGCCGACCATATCGAGGTGGTGTGGGATCTCGACCACGAGTTGCGATTACAAGCCGAGGCAGCGGGCATCGCGTACGCCCGGGCCAGCACCCCCAATGCCGACCCGCGGTTCGCTCGACTAGCCAGAGGTTTGATCGACGAACTCCGTTACGGCCGTATACCTGCGCGGGTGAGTGGCCCCGATCCGGTGCCGGGCTGTCTGTCCAGCATCAACGGCCAGCCATGCCGTCCGCCGCACTGCGTGGCTAGCGTCAGTCCGGCCAGGCCGAGTGCAGGATCGCCGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the biosynthesis of heme. Catalyzes the ferrous insertion into protoporphyrin IX to form protoheme. {ECO:0000269|PubMed:11948160, ECO:0000269|PubMed:15850757}.

Family

Ferrochelatase family

GO

metal ion binding GO:0046872 molecular_function
plasma membrane GO:0005886 cellular_component
cytoplasm GO:0005737 cellular_component
2 iron, 2 sulfur cluster binding GO:0051537 molecular_function
ferrochelatase activity GO:0004325 molecular_function
heme biosynthetic process GO:0006783 biological_process

InterPro

UniProt: P9WNE3
GenBank: Rv1485
EnsemblBacteria: Rv1485
Mycobrowser: Rv1485

Summary

Name: Ferrochelatase (EC 4.99.1.1) (Heme synthase) (Protoheme ferro-lyase)
Family: Ferrochelatase family
Protein Sequence: MQFDAVLLLSFGGPEGPEQVRPFLENVTRGRGVPAERLDAVAEHYLHFGGVSPINGINRTLIAELEAQQELPVYFGNRNWEPYVEDAVTAMRDNGVRRAAVFATSAWSGYSSCTQYVEDIARARRAAGRDAPELVKLRPYFDHPLFVEMFADAITAAAATVRGDARLVFTAHSIPTAADRRCGPNLYSRQVAYATRLVAAAAGYCDFDLAWQSRSGPPQVPWLEPDVTDQLTGLAGAGINAVIVCPIGFVADHIEVVWDLDHELRLQAEAAGIAYARASTPNADPRFARLARGLIDELRYGRIPARVSGPDPVPGCLSSINGQPCRPPHCVASVSPARPSAGSP
Mass: 37,144 Da
Length: 344 Aa

Rv1485 doesn't seem to be a targeted by any drug.

Porphyrin and chlorophyll metabolism mtu00860
mtu00860
PATHWAY_MAP mtu00860 Porphyrin and chlorophyll metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
HemZ is essential for heme biosynthesis in Mycobacterium tuberculosis.: Tuberculosis (Edinb). 2005 May;85(3):197-204. doi: 10.1016/j.tube.2005.01.002.
Identification of [2Fe-2S] clusters in microbial ferrochelatases.: J Bacteriol. 2002 May;184(9):2460-4. doi: 10.1128/jb.184.9.2460-2464.2002.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.