Gene - combatTB X

pks5

Type: protein_coding
Name: pks5
Locus Name: Rv1527c
Product: Probable polyketide synthase Pks5
Functional Category: Lipid metabolism
Location: 1722083..1728409 (- strand)
Gene Length: 6326 bp
Nucleotides: GTGGGTAAGGAGAGAACAAAGACCGTGGATAGGACACGTGTTACGCCGGTAGCTGTCATTGGTATGGGGTGCCGGCTGCCGGGGGGCATCGACTCACCCGATCGGTTGTGGGAGGCGTTGCTGCGGGGCGACGATCTGGTCACCGAGATCCCCGCCGACCGCTGGGACATCGACGAGTACTACGACCCCGAACCCGGCGTGCCCGGACGCACCGACTGCAAATGGGGCGCGTACCTCGATAACGTCGGCGACTTTGATCCCGAGTTCTTCGGGATCGGGGAGAAAGAAGCGATAGCGATCGATCCGCAGCACCGCTTGTTGCTGGAAACCTCCTGGGAAGCCATGGAGCACGGCGGCCTAACACCGAACCAGATGGCCTCGCGGACAGGGGTTTTCGTGGGGTTGGTTCATACCGACTACATATTGGTACACGCCGACAACCAGACTTTCGAAGGGCCGTACGGCAACACGGGCACCAATGCCTGCTTCGCATCCGGGCGGGTGGCGTACGCCATGGGCTTGCAGGGTCCCGCGATCACGGTAGATACCGCATGCTCGTCTGGCCTCACCGCTATCCACCTGGCTTGTCGTAGCCTGCACGACGGCGAAAGCGACATTGCGCTAGCCGGCGGTGTCTACGTGATGCTCGAACCGCGGCGGTTCGCCTCGGGCTCGGCGTTGGGCATGTTGTCTGCGACCGGGCGCTGTCATGCGTTCGACGTTTCCGCGGACGGTTTCGTCTCCGGTGAGGGTTGTGTCATGTTGGCGCTCAAGCGGTTGCCGGATGCGCTGGCCGATGGGGACCGGATCCTGGCCGTAATTCGTGGCACGGCCGCCAACCAAGACGGCCACACCGTCAATATCGCGACACCATCACGGTCCGCGCAGGTCGCGGCGTATCGGGAGGCGTTGGATGTGGCCGGTGTGGACCCGGCTACGGTGGGCATGGTCGAGGCGCACGGCCCGGGCACCCCGGTGGGTGACCCCATCGAATACGCCAGCCTGGCCGAGGTATACGGCAACGACGGCCCCTGCGCGCTGGCATCGGTGAAGACCAATTTCGGCCACACCCAGTCGGCCGCTGGAGCGCTGGGACTGATGAAGGCGGTCCTGGCCCTCCAACACGGCGTGGTCCCACAGAATCTGCACTTCACGGCCCTGCCTGACAAGCTTGCCGCAATCGAAACCAACCTGTTTGTGCCGCAAGAGATTACGCCGTGGCCCGGCGCCGATCAAGAAACGCCCCGGCGCGCGGCGGTGTCGTCGTATGGCATGACGGGCACCAATGTGCACGCCATTGTCGAGCAGGCACCGGTGCCAGCCCCCGAATCCGGTGCACCAGGCGACACCCCGGCCACACCCGGTATCGACGGCGCGCTGCTGTTCGCGCTGTCGGCCAGCTCGCAGGACGCGCTGCGGCAGACCGCCGCGCGGCTGGCCGATTGGGTCGACGCCCAAGGACCCGAGCTGGCGCCGGCGGATCTGGCCTACACCCTGGCCCGCCGGCGCGGACACCGGCCGGTGCGCACCGCCGTACTGGCCGCCACCACCGCGGAGCTGACCGAGGCGCTGCGGGAAGTCGCCACCGGCGAACCCCCCTACCCACCCGCGGTCGGCCAAGACGACCGCGGACCGGTCTGGGTGTTCTCCGGGCAAGGCTCGCAATGGGCGGGCATGGGCGCCGACCTGCTGGCCACCGAACCGGTATTCGCCGCCACCATCGCCGCGATCGAACCACTGATCGCCGCGGAATCCGGCTTCTCGGTGACCGAAGCCATGACCGCCCCCGAGGTCGTGACCGGCATCGACCGGGTGCAACCGACCCTGTTTGCCATGCAGGTCGCGCTGGCGGCCACAATGAAGTCCTACGGCGTAGCGCCCGGCGCGGTCATCGGCCACTCGCTGGGCGAGTCCGCGGCCGCGGTGGTCGCCGGCGCGCTCTGCCTCGAAGACGGAGTGCGCGTCATCTGCCGGCGGTCGGCGCTGATGACCCGTATCGCCGGCGCCGGCGCCATGGCATCGGTGGAACTGCCTGCTCAACAAGTGCTTTCGGAGTTGATGGCGCGCGGCGTCAACGACGCCGTGGTCGCGGTGGTGGCCTCCCCGCAGTCCACCGTGATCGGCGGGGCCACCCAGACGGTACGCGACCTGGTGGCCGCCTGGGAGCAACGCGACGTGCTGGCCCGTGAGGTGGCCGTCGACGTAGCATCGCACTCCCCGCAGGTCGACCCGATCCTCGACGAACTGGCCGAAGCGCTCGCCGAAATCAGCCCGCTGCAACCGGAAATCCCCTACTACTCGGCGACCTCGTTCGACCCGCGCGAGGAGCCCTACTGCGACGCCTACTACTGGGTGGACAACCTGCGCCACACCGTACGCTTCGCCGCGGCGGTGCAAGCCGCCCTCGAAGACGGCTACCGGGTCTTCACCGAGCTGACCCCCCACCCCCTGCTGACCCACGCGGTCGACCAGACCGCCCGCAGCCTCGACATGTCCGCGGCTGCGCTGGCCGGCATGCGCCGCGAACAACCACTGCCCCACGGGCTGCGCGCACTGGCCGGCGACCTCTACGCCGCCGGCGCCGCCGTCGACTTCGCCGTGCTCTACCCCACCGGCCGACTGATCAACGCACCACTGCCCACATGGAACCACCGTCGTCTGCTGCTCGACGACACGACCCGTCGCATCGCCCACGCCAATACCGTCGCCGTACACCCATTGCTGGGCTCCCACGTGCGCCTGCCCGAGGAGCCGGAACGCCACGTCTGGCAGGGCGAGGTCGGCACCGTGACACAACCCTGGCTGGCCGATCACCAAATCCACGGCGCGGCCGCCCTTCCGGGAGCCGCCTACTGCGAGATGGCGTTGGCCGCAGCCCGCGCCGTCCTCGGCGAAGCCTCCGAAGTCCGCGACATCCGATTCGAGCAGATGTTGCTGCTCGACGACGAAACCCCCATCGGCGTCACCGCCACGGTAGAAGCCCCCGGCGTTGTCCCACTCACAGTAGAGACCAGCCATGATGGTAGGTACACACGGCAACTCGCCGCGGTTCTGCACGTTGTCCGGGAAGCCGACGACGCCCCGGACCAGCCACCCCAGAAAAACATCGCCGAACTGCTGGCCAGCCATCCCCACAAGGTGGACGGCGCCGAGGTTCGGCAATGGCTGGACAAGCGTGGTCATCGCTTAGGGCCGGCTTTTGCGGGTCTGGTTGACGCGTACATCGCCGAAGGGGCCGGCGACACGGTGCTGGCCGAGGTCAACCTACCGGGCCCGCTTCGCTCGCAGGTCAAAGCTTACGGCGTGCATCCCGTGCTGCTGGATGCGTGCTTCCAGTCGGTGGCGGCTCATCCCGCCGTCCAGGGTATGGCCGACGGTGGTTTGTTGCTGCCGTTGGGTGTGCGGCGGTTGCGGTCCTATGGGTCGGCCCGTCATGCCCGGTACTGCTGCACGACGGTGACCGCGTGCGGTGTGGGCGTGGAAGCCGACCTGGACGTGTTGGACGAGCACGGCGCGGTGGTGTTGGCGGTGCGTGGGCTGCAGTTGGGTACCGGCGCGTCGCAGGCCAGCGAACGTGCCCGGGTTTTGGGTGAGCGGCTGCTGAGCATCGAATGGCACGAACGTGAGCTGCCCGAAAACAGCCACGCCGAACCCGGCGCCTGGCTGCTGATCAGCACGTGTGACGCTACGGATTTGGTGGCCGCACAGTTGACCGACGCGCTGAAGGTGCACGACGCGCAGTGCACAACGATGTCCTGGCCGCAGCGCGCCGACCACGCAGCCCAGGCCGCGCGGCTGCGCGACCAGCTCGGCACCGGCGGGTTCACCGGCGTATTCGTGTTGACCGCGCCGCAGACCGGCGACCCCGACGCGGAATCCCCCGTGCGCGGCGGCGAGCTTGTCAAACATGTGGTGCGTATCGCCCGCGAGATTCCAGAGATCACGGCCCAAGAGCCGCGGTTGTATGTGCTGACCCACAATGCTCAGGCGGTGTTGTCTGGTGACCGACCCAATCTTGAGCAGGGTGGGATGCGGGGGTTGTTGCGGGTGATTGGTGCTGAGCATCCGCATTTGAAGGCCAGCTATGTCGATGTGGATGAGCAGACCGGCGCGGAGTCGGTGGCGCGTCAGCTGCTGGCGGCCAGCGGCGAGGATGAGACCGCCTGGCGCAACGACCAGTGGTACACCGCCCGGTTGTGCCCAGCCCCGCTGCGCCCCGAGGAGCGGCAAACCACCGTCGTCGACCACGCCGAGGCCGGCATGCGCCTACAGATCCGTACTCCCGGTGACCTGCAAACCTTGGAGTTCGCCGCGTTCGACCGGGTCCCGCCGGGCCCGGGAGAGATCGAGGTCGCCGTCACCGCGTCCAGCATCAACTTCGCCGACGTGCTGGTCACCTTCGGCCGCTACCAAACCCTCGACGGGCGACAGCCGCAGCTGGGCACCGATTTCGCCGGGGTGGTGAGCGCGGTCGGGCCCGGGGTCAGCGAACTCAAGGTCGGTGACCGGGTCGGCGGCATGTCACCCAACGGCTGCTGGGCCACGTTCGTCACCTGCGACGCCCGCCTGGCCACCAGACTGCCCGAGGGGCTGACCGACGCCCAAGCCGCGGCAGTGACCACCGCCAGCGCCACCGCGTGGTACGGGCTGCAGGACCTGGCGCGGATCAAGGCCGGCGACAAGGTGCTCATCCACTCCGCCACCGGCGGAGTGGGCCAGGCCGCAATCGCGATCGCGCGTGCGGCCGGAGCCCAGATCTACGCCACCGCAGGCAACGAAAAGCGTCGAGACCTGCTGCGCGACATGGGCATCGAACACGTCTATGACTCGCGCAGCGTCGAGTTCGCCGAGCAGATCCGCCGCGACACCGCCGGCTACGGCGTGGACATCGTGCTCAACTCCGTGACCGGTGCCGCACAGTTGGCCGGCCTCAAACTACTGGCCCTGGGCGGCCGCTTCATCGAAATCGGCAAACGCGACATCTACTCCAATACCCGACTGGAACTCTTGCCATTCCGGCGCAATCTGGCGTTCTACGGTCTAGACCTGGGGTTGATGTCGGTGAGCCATCCGGCTGCGGTCCGCGAACTGTTGAGCACGGTGTATCGGTTGACCGTCGAGGGTGTGCTGCCGATGCCGCAGAGCACCCATTACCCGCTGGCCGAGGCGGCCACCGCGATCCGGGTGATGGGCGCGGCCGAGCACACCGGCAAGCTCATCCTCGACGTCCCCCACGCCGGGCGCAGCAGCGTGGTGCTTCCCCCCGAACAGGCTCGGGTTTTCCGTTCAGACGGGTCCTACATCATCACCGGTGGCCTGGGTGGGCTGGGCTTATTCCTGGCCGAGAAGATGGCCAACGCCGGGGCCGGCCGCATCGTGCTCAGCTCGCGCTCACAGCCCAGCCAAAAGGCGTTGGAGACCATCGAACTCGTCCGCGCGATCGGGTCTGACGTAGTGGTGGAGTGCGGCGATATCGCCCAGCCGGACACCGCCGACCGGTTGGTCACGGCGGCCACCGCGACGGGTTTGCCGCTGCGCGGCGTGCTGCACGCGGCCGCGGTGGTCGAGGACGCCACCTTGGCCAACATCACCGACGAACTCATCGAACGCGACTGGGCGCCCAAGGCATATGGCGCCTGGCAACTGCACCGGGCCACCGCGGACCAGCCGCTGGACTGGTTCTGCTCATTCTCCTCAGCGGCCGCCCTGGTGGGCTCGCCGGGTCAGGGCGCCTACGCCGCGGCCAACAGCTGGCTGGACACCTTCACCCACTGGCGGCGAGCCCAAGACCTACCAGCCACCTCCATCGCCTGGGGCGCCTGGGGCCAGATCGGCCGCGCGATCGCCTTTGCCGAACAAACCGGCGACGCCATCGCCCCCGAGGAAGGCGCCTACGCCTTCGAGACCCTGCTGCGCCACAACCGCGCCTACAGCGGCTACGCCCCGGTGATCGGATCACCCTGGCTGACAGCCTTCGCCCAGCACAGCCCATTCGCGGAGAAGTTCCAATCCCTGGGGCAAAACCGTTCAGGCACAAGCAAATTCCTTGCCGAGCTGGTGGACCTGCCCCGCGAGGAATGGCCAGACCGGCTGCGGCGGCTACTCTCCAAGCAGGTCGGCCTAATCCTACGCCGCACCATCGACACCGACCGGCTGCTCAGCGAGTACGGCCTGGACTCACTGAGCAGCCAGGAACTGCGTGCGCGGGTGGAAGCCGAAACCGGGATCCGCATTTCCGCCACCGAAATCAACACCACCGTTCGTGGCCTGGCGGATCTGATGTGCGACAAGTTGGCGGCCGACAGGGACGCGCCGGCACCCGCCTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Polyketide synthase likely involved in the biosynthesis of a polymethyl-branched fatty acid (PMB-FA) that might only be produced during host infection. Is required for the full virulence of M.tuberculosis during host infection. {ECO:0000269|PubMed:12855735, ECO:0000305}.

Family

Unknown

GO

pathogenesis GO:0009405 biological_process
cell wall GO:0005618 cellular_component
cytosol GO:0005829 cellular_component
phosphopantetheine binding GO:0031177 molecular_function
plasma membrane GO:0005886 cellular_component
oxidoreductase activity GO:0016491 molecular_function
transferase activity, transferring acyl groups GO:0016746 molecular_function
fatty acid biosynthetic process GO:0006633 biological_process

InterPro

UniProt: O53901
GenBank: Rv1527c
EnsemblBacteria: Rv1527c
Mycobrowser: Rv1527c

Summary

Name: Mycocerosic acid synthase-like polyketide synthase (MAS-like PKS) (EC 2.3.1.-) (Polyketide synthase Pks5)
Family: Unknown
Protein Sequence: MGKERTKTVDRTRVTPVAVIGMGCRLPGGIDSPDRLWEALLRGDDLVTEIPADRWDIDEYYDPEPGVPGRTDCKWGAYLDNVGDFDPEFFGIGEKEAIAIDPQHRLLLETSWEAMEHGGLTPNQMASRTGVFVGLVHTDYILVHADNQTFEGPYGNTGTNACFASGRVAYAMGLQGPAITVDTACSSGLTAIHLACRSLHDGESDIALAGGVYVMLEPRRFASGSALGMLSATGRCHAFDVSADGFVSGEGCVMLALKRLPDALADGDRILAVIRGTAANQDGHTVNIATPSRSAQVAAYREALDVAGVDPATVGMVEAHGPGTPVGDPIEYASLAEVYGNDGPCALASVKTNFGHTQSAAGALGLMKAVLALQHGVVPQNLHFTALPDKLAAIETNLFVPQEITPWPGADQETPRRAAVSSYGMTGTNVHAIVEQAPVPAPESGAPGDTPATPGIDGALLFALSASSQDALRQTAARLADWVDAQGPELAPADLAYTLARRRGHRPVRTAVLAATTAELTEALREVATGEPPYPPAVGQDDRGPVWVFSGQGSQWAGMGADLLATEPVFAATIAAIEPLIAAESGFSVTEAMTAPEVVTGIDRVQPTLFAMQVALAATMKSYGVAPGAVIGHSLGESAAAVVAGALCLEDGVRVICRRSALMTRIAGAGAMASVELPAQQVLSELMARGVNDAVVAVVASPQSTVIGGATQTVRDLVAAWEQRDVLAREVAVDVASHSPQVDPILDELAEALAEISPLQPEIPYYSATSFDPREEPYCDAYYWVDNLRHTVRFAAAVQAALEDGYRVFTELTPHPLLTHAVDQTARSLDMSAAALAGMRREQPLPHGLRALAGDLYAAGAAVDFAVLYPTGRLINAPLPTWNHRRLLLDDTTRRIAHANTVAVHPLLGSHVRLPEEPERHVWQGEVGTVTQPWLADHQIHGAAALPGAAYCEMALAAARAVLGEASEVRDIRFEQMLLLDDETPIGVTATVEAPGVVPLTVETSHDGRYTRQLAAVLHVVREADDAPDQPPQKNIAELLASHPHKVDGAEVRQWLDKRGHRLGPAFAGLVDAYIAEGAGDTVLAEVNLPGPLRSQVKAYGVHPVLLDACFQSVAAHPAVQGMADGGLLLPLGVRRLRSYGSARHARYCCTTVTACGVGVEADLDVLDEHGAVVLAVRGLQLGTGASQASERARVLGERLLSIEWHERELPENSHAEPGAWLLISTCDATDLVAAQLTDALKVHDAQCTTMSWPQRADHAAQAARLRDQLGTGGFTGVFVLTAPQTGDPDAESPVRGGELVKHVVRIAREIPEITAQEPRLYVLTHNAQAVLSGDRPNLEQGGMRGLLRVIGAEHPHLKASYVDVDEQTGAESVARQLLAASGEDETAWRNDQWYTARLCPAPLRPEERQTTVVDHAEAGMRLQIRTPGDLQTLEFAAFDRVPPGPGEIEVAVTASSINFADVLVTFGRYQTLDGRQPQLGTDFAGVVSAVGPGVSELKVGDRVGGMSPNGCWATFVTCDARLATRLPEGLTDAQAAAVTTASATAWYGLQDLARIKAGDKVLIHSATGGVGQAAIAIARAAGAQIYATAGNEKRRDLLRDMGIEHVYDSRSVEFAEQIRRDTAGYGVDIVLNSVTGAAQLAGLKLLALGGRFIEIGKRDIYSNTRLELLPFRRNLAFYGLDLGLMSVSHPAAVRELLSTVYRLTVEGVLPMPQSTHYPLAEAATAIRVMGAAEHTGKLILDVPHAGRSSVVLPPEQARVFRSDGSYIITGGLGGLGLFLAEKMANAGAGRIVLSSRSQPSQKALETIELVRAIGSDVVVECGDIAQPDTADRLVTAATATGLPLRGVLHAAAVVEDATLANITDELIERDWAPKAYGAWQLHRATADQPLDWFCSFSSAAALVGSPGQGAYAAANSWLDTFTHWRRAQDLPATSIAWGAWGQIGRAIAFAEQTGDAIAPEEGAYAFETLLRHNRAYSGYAPVIGSPWLTAFAQHSPFAEKFQSLGQNRSGTSKFLAELVDLPREEWPDRLRRLLSKQVGLILRRTIDTDRLLSEYGLDSLSSQELRARVEAETGIRISATEINTTVRGLADLMCDKLAADRDAPAPA
Mass: 223,889 Da
Length: 2108 Aa

Rv1527c doesn't seem to be a targeted by any drug.

R-MTU-9635470 R-MTU-9635470
R-MTU-9635470
R-MTU-9635470

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Virulence attenuation of two Mas-like polyketide synthase mutants of Mycobacterium tuberculosis.: Microbiology. 2003 Jul;149(Pt 7):1837-47. doi: 10.1099/mic.0.26278-0.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.