Gene - combatTB X

ileS

Type: protein_coding
Name: ileS
Locus Name: Rv1536
Product: Isoleucyl-tRNA synthetase IleS
Functional Category: Information pathways
Location: 1736519..1739644 (+ strand)
Gene Length: 3125 bp
Nucleotides: TGACCGATAACGCATATCCAAAGCTGGCCGGCGGGGCACCCGACCTCCCGGCACTCGAACTCGAGGTCCTCGACTACTGGTCCCGTGACGACACCTTCCGGGCCAGCATTGCTCGCCGCGATGGCGCCCCCGAGTATGTGTTCTATGACGGGCCGCCGTTTGCCAACGGTCTGCCGCATTATGGGCACCTGCTCACCGGCTACGTCAAAGACATCGTGCCGCGATATCGCACTATGCGCGGTTACAAGGTGGAGCGTCGCTTCGGCTGGGACACTCACGGGCTGCCCGCCGAACTCGAAGTCGAGCGCCAGCTTGGCATCACTGACAAATCCCAGATCGAGGCCATGGGTATCGCCGCCTTCAACGATGCCTGCCGCGCATCCGTGTTGCGCTACACCGACGAGTGGCAGGCGTATGTAACTCGGCAAGCTCGCTGGGTCGACTTCGACAACGATTACAAGACGCTCGATCTGGCTTACATGGAGTCGGTGATTTGGGCCTTCAAACAGTTGTGGGACAAGGGCCTGGCCTACGAGGGCTACCGGGTGCTGCCGTACTGCTGGCGCGACGAAACTCCGCTGTCGAATCACGAACTGCGGATGGACGACGACGTCTACCAAAGCCGCCAAGATCCCGCGGTAACGGTGGGCTTCAAGGTGGTGGGTGGCCAACCAGACAACGGGCTAGACGGTGCCTACTTGCTGGTGTGGACGACGACTCCGTGGACCCTGCCGTCGAACCTCGCAGTTGCGGTAAGCCCGGACATCACCTACGTACAGGTCCAGGCGGGCGATCGCCGTTTCGTACTGGCCGAGGCACGGCTGGCCGCTTACGCCCGCGAACTCGGTGAAGAGCCCGTGGTGCTCGGCACCTATCGCGGCGCCGAACTGCTGGGCACCCGCTACCTGCCGCCGTTTGCCTATTTCATGGACTGGCCCAACGCTTTTCAGGTGCTAGCAGGCGACTTTGTAACGACCGACGATGGCACCGGCATCGTGCATATGGCACCGGCCTATGGTGAGGACGACATGGTGGTCGCGGAGGCGGTCGGTATCGCGCCGGTGACTCCGGTCGACTCCAAGGGACGCTTCGACGTCACCGTTGCCGATTACCAAGGGCAGCATGTCTTTGACGCCAACGCGCAGATCGTCCGGGACCTGAAGACCCAAAGCGGCCCGGCTGCGGTGAATGGCCCAGTGTTGATTCGTCACGAAACCTACGAGCACCCTTACCCACACTGCTGGCGATGCCGTAACCCGCTGATCTACCGGTCGGTGTCGTCGTGGTTCGTCAGGGTGACGGACTTCCGAGACCGCATGGTGGAGCTAAACCAGCAGATCACGTGGTATCCCGAACACGTCAAGGACGGCCAGTTCGGCAAGTGGCTGCAGGGCGCCCGCGATTGGTCGATCTCCCGGAATCGCTACTGGGGTACCCCGATTCCGGTATGGAAGTCCGACGACCCGGCCTACCCGCGCATCGATGTCTACGGCAGCCTCGACGAGCTGGAGCGCGACTTCGGCGTACGCCCGGCCAATTTGCACCGGCCCTACATCGACGAGCTCACCCGTCCCAACCCAGACGATCCGACTGGCCGTAGCACGATGCGACGCATTCCCGATGTGCTCGACGTGTGGTTCGACTCGGGATCCATGCCGTATGCCCAGGTGCACTACCCGTTCGAGAACCTGGATTGGTTCCAGGGACACTACCCCGGCGACTTCATCGTCGAGTACATCGGGCAGACCCGTGGCTGGTTTTACACACTGCATGTGTTGGCGACCGCGCTCTTTGACCGGCCGGCATTCAAAACCTGTGTGGCGCATGGGATTGTCCTTGGTTTCGATGGCCAGAAGATGAGCAAGTCGCTGCGCAACTATCCAGACGTAACAGAGGTGTTCGATCGCGACGGCTCCGACGCCATGCGGTGGTTCCTGATGGCATCGCCGATTCTGCGCGGCGGCAACCTGATCGTCACTGAGCAAGGAATTCGCGACGGTGTGCGACAAGTCCTGCTGCCCCTGTGGAACACCTACAGCTTCCTGGCGCTGTATGCACCGAAAGTCGGTACCTGGCGCGTCGATTCGGTGCACGTGCTGGATCGCTATATCCTGGCCAAGCTGGCGGTGCTGCGCGACGACCTCAGCGAGTCGATGGAAGTTTACGATATTCCCGGTGCCTGTGAACATTTGCGTCAGTTCACTGAGGCGTTGACTAATTGGTATGTGCGACGGTCGCGTTCGCGGTTCTGGGCAGAAGACGCCGATGCCATCGACACGCTACACACCGTGTTGGAGGTGACCACGAGGCTGGCCGCCCCGCTGCTTCCGCTGATCACCGAGATAATCTGGCGTGGTCTGACACGCGAGCGATCGGTGCACCTGACGGACTGGCCAGCGCCCGACCTGCTGCCGTCGGATGCCGACCTGGTCGCCGCGATGGACCAGGTCCGCGACGTGTGCTCGGCGGCATCCTCGCTGCGCAAGGCCAAGAAGCTACGGGTGCGCCTGCCGCTACCGAAACTCATTGTGGCAGTTGAGAATCCGCAACTTCTGAGGCCGTTCGTCGACCTCATTGGCGACGAGCTTAACGTGAAGCAGGTCGAACTGACCGATGCCATCGACACCTATGGCCGATTCGAGCTCACGGTCAACGCCCGGGTAGCCGGACCACGGCTGGGCAAAGATGTGCAGGCCGCCATCAAGGCGGTCAAGGCCGGCGACGGCGTCATAAACCCGGACGGCACCTTGTTGGCGGGCCCCGCGGTGCTGACGCCCGACGAGTACAACTCCCGGCTGGTGGCCGCCGACCCGGAGTCCACCGCGGCGTTGCCCGACGGCGCCGGGCTGGTCGTTCTGGATGGCACCGTCACTGCCGAACTCGAAGCCGAGGGCTGGGCCAAAGATCGCATCCGCGAACTGCAAGAGCTGCGTAAGTCGACCGGGCTGGACGTTTCCGACCGCATCCGGGTGGTGATGTCGGTGCCTGCGGAACGCGAAGACTGGGCGCGCACCCATCGCGACCTCATTGCCGGAGAAATCTTGGCTACCGACTTCGAATTCGCCGACCTCGCCGATGGTGTGGCCATCGGCGACGGCGTGCGGGTAAGCATCGAAAAGACCTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity). {ECO:0000250}.; FUNCTION: Confers high-level resistance to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog that competitively inhibits activation by Ile-tRNA synthetase, thus inhibiting protein biosynthesis.

Family

Class-I aminoacyl-tRNA synthetase family, IleS type 2 subfamily

GO

response to antibiotic GO:0046677 biological_process
cell wall GO:0005618 cellular_component
ATP binding GO:0005524 molecular_function
plasma membrane GO:0005886 cellular_component
cytoplasm GO:0005737 cellular_component
zinc ion binding GO:0008270 molecular_function
isoleucine-tRNA ligase activity GO:0004822 molecular_function
isoleucyl-tRNA aminoacylation GO:0006428 biological_process
aminoacyl-tRNA editing activity GO:0002161 molecular_function
tRNA binding GO:0000049 molecular_function

InterPro

UniProt: P9WFV3
GenBank: Rv1536
EnsemblBacteria: Rv1536
Mycobrowser: Rv1536

Summary

Name: Isoleucine--tRNA ligase (EC 6.1.1.5) (Isoleucyl-tRNA synthetase) (IleRS)
Family: Class-I aminoacyl-tRNA synthetase family, IleS type 2 subfamily
Protein Sequence: MTDNAYPKLAGGAPDLPALELEVLDYWSRDDTFRASIARRDGAPEYVFYDGPPFANGLPHYGHLLTGYVKDIVPRYRTMRGYKVERRFGWDTHGLPAELEVERQLGITDKSQIEAMGIAAFNDACRASVLRYTDEWQAYVTRQARWVDFDNDYKTLDLAYMESVIWAFKQLWDKGLAYEGYRVLPYCWRDETPLSNHELRMDDDVYQSRQDPAVTVGFKVVGGQPDNGLDGAYLLVWTTTPWTLPSNLAVAVSPDITYVQVQAGDRRFVLAEARLAAYARELGEEPVVLGTYRGAELLGTRYLPPFAYFMDWPNAFQVLAGDFVTTDDGTGIVHMAPAYGEDDMVVAEAVGIAPVTPVDSKGRFDVTVADYQGQHVFDANAQIVRDLKTQSGPAAVNGPVLIRHETYEHPYPHCWRCRNPLIYRSVSSWFVRVTDFRDRMVELNQQITWYPEHVKDGQFGKWLQGARDWSISRNRYWGTPIPVWKSDDPAYPRIDVYGSLDELERDFGVRPANLHRPYIDELTRPNPDDPTGRSTMRRIPDVLDVWFDSGSMPYAQVHYPFENLDWFQGHYPGDFIVEYIGQTRGWFYTLHVLATALFDRPAFKTCVAHGIVLGFDGQKMSKSLRNYPDVTEVFDRDGSDAMRWFLMASPILRGGNLIVTEQGIRDGVRQVLLPLWNTYSFLALYAPKVGTWRVDSVHVLDRYILAKLAVLRDDLSESMEVYDIPGACEHLRQFTEALTNWYVRRSRSRFWAEDADAIDTLHTVLEVTTRLAAPLLPLITEIIWRGLTRERSVHLTDWPAPDLLPSDADLVAAMDQVRDVCSAASSLRKAKKLRVRLPLPKLIVAVENPQLLRPFVDLIGDELNVKQVELTDAIDTYGRFELTVNARVAGPRLGKDVQAAIKAVKAGDGVINPDGTLLAGPAVLTPDEYNSRLVAADPESTAALPDGAGLVVLDGTVTAELEAEGWAKDRIRELQELRKSTGLDVSDRIRVVMSVPAEREDWARTHRDLIAGEILATDFEFADLADGVAIGDGVRVSIEKT
Mass: 117,340 Da
Length: 1041 Aa

Rv1536 doesn't seem to be a targeted by any drug.

Rv1536 doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
A eubacterial Mycobacterium tuberculosis tRNA synthetase is eukaryote-like and resistant to a eubacterial-specific antisynthetase drug.: Biochemistry. 1996 Aug 6;35(31):9995-10003. doi: 10.1021/bi9603027.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.