Gene - combatTB X

dnaE1

Type: protein_coding
Name: dnaE1
Locus Name: Rv1547
Product: Probable DNA polymerase III (alpha chain) DnaE1 (DNA nucleotidyltransferase)
Functional Category: Information pathways
Location: 1747694..1751248 (+ strand)
Gene Length: 3554 bp
Nucleotides: TGAGCGGTTCATCTGCGGGGTCCTCCTTCGTGCACCTGCACAACCACACCGAGTATTCGATGCTGGACGGTGCCGCGAAGATCACGCCCATGCTCGCCGAGGTGGAGCGGCTGGGGATGCCCGCGGTGGGGATGACCGACCACGGAAACATGTTCGGTGCCAGCGAGTTCTACAACTCCGCGACCAAGGCCGGGATCAAGCCGATCATCGGCGTGGAGGCATACATCGCGCCGGGCTCGCGGTTCGACACCCGGCGCATCCTGTGGGGTGACCCCAGCCAAAAGGCCGACGACGTCTCCGGCAGCGGCTCCTACACGCACCTGACGATGATGGCCGAGAACGCCACCGGTCTGCGCAACCTGTTCAAGCTGTCCTCGCATGCTTCCTTCGAGGGCCAGCTGAGCAAGTGGTCGCGCATGGACGCCGAGCTCATCGCCGAACACGCCGAGGGCATCATCATCACCACCGGATGCCCGTCGGGGGAGGTGCAGACCCGCCTGCGGCTCGGCCAGGATCGGGAGGCGCTCGAAGCCGCGGCGAAGTGGCGGGAGATCGTCGGACCGGACAACTACTTCCTTGAGCTGATGGACCACGGGCTGACCATCGAACGCCGGGTCCGTGACGGTCTGCTCGAGATCGGACGCGCGCTCAACATTCCGCCTCTTGCCACCAATGACTGCCACTACGTGACCCGCGACGCCGCCCACAACCATGAGGCTTTGTTGTGTGTGCAGACCGGCAAGACCCTCTCGGATCCGAATCGCTTCAAGTTCGACGGTGACGGCTACTACCTGAAGTCGGCCGCCGAGATGCGCCAGATCTGGGACGACGAAGTGCCGGGCGCGTGTGACTCCACCTTGTTGATCGCCGAACGGGTGCAGTCCTACGCCGACGTGTGGACACCGCGCGACCGGATGCCCGTGTTTCCGGTGCCCGATGGGCATGACCAGGCGTCCTGGCTGCGTCACGAGGTGGACGCCGGGCTTCGCCGGCGATTTCCGGCCGGTCCGCCGGACGGGTACCGCGAGCGCGCCGCCTACGAGATCGACGTCATCTGCTCCAAAGGTTTCCCATCGTACTTTCTGATCGTCGCCGACCTGATCAGCTACGCGCGGTCGGCGGGCATAAGGGTGGGTCCCGGCCGCGGCTCGGCCGCCGGCTCGCTGGTCGCCTACGCGCTGGGCATCACCGACATCGACCCGATTCCACACGGTCTGCTGTTCGAGCGGTTCCTCAACCCCGAGCGCACCTCGATGCCCGACATCGATATCGACTTCGACGACCGGCGCCGCGGTGAGATGGTGCGCTACGCAGCCGACAAGTGGGGCCACGACCGGGTCGCGCAGGTCATCACCTTCGGCACCATCAAAACCAAAGCGGCGCTGAAGGATTCGGCGCGAATCCACTACGGGCAGCCCGGGTTCGCCATCGCCGACCGGATCACCAAGGCGTTGCCGCCGGCGATCATGGCCAAAGACATCCCGCTGTCTGGGATCACCGATCCCAGCCACGAACGGTACAAGGAGGCCGCCGAGGTCCGCGGCCTGATCGAAACCGACCCGGACGTACGCACCATCTACCAGACCGCACGCGGGTTGGAAGGCCTGATCCGCAACGCGGGTGTGCACGCCTGCGCGGTGATCATGAGCAGCGAGCCGCTGACTGAGGCCATCCCGTTGTGGAAGCGGCCGCAGGACGGGGCCATCATCACCGGCTGGGATTACCCGGCGTGCGAGGCCATCGGTCTGCTGAAAATGGACTTCCTGGGCCTGCGGAACCTGACGATCATCGGCGACGCGATCGACAACGTCAGGGCCAACAGGGGTATCGACCTCGACCTGGAATCCGTGCCGCTGGACGACAAGGCCACCTATGAGCTGCTGGGCCGCGGCGACACCCTGGGCGTGTTCCAGCTCGACGGCGGGCCCATGCGCGACCTGCTGCGCCGCATGCAGCCGACCGGGTTCGAAGACGTCGTCGCCGTTATCGCGCTGTACCGGCCCGGCCCGATGGGCATGAACGCACACAACGACTATGCCGACCGCAAGAACAACCGGCAGGCCATCAAACCTATTCACCCGGAACTCGAAGAACCGCTGCGCGAGATCCTCGCCGAGACCTACGGCCTCATCGTCTATCAAGAGCAGATCATGCGCATCGCGCAGAAGGTGGCGAGCTACTCGTTGGCCCGCGCCGACATTCTACGCAAGGCCATGGGCAAGAAGAAACGCGAGGTGCTGGAGAAGGAGTTCGAGGGCTTCTCCGATGGCATGCAGGCCAACGGGTTCTCTCCGGCGGCCATCAAGGCGCTGTGGGACACCATCCTGCCGTTCGCTGACTACGCGTTCAACAAGTCACATGCCGCCGGCTACGGCATGGTGTCCTACTGGACGGCCTACCTCAAGGCCAACTATCCCGCCGAGTACATGGCCGGTCTGTTGACGTCGGTCGGCGACGATAAAGACAAGGCCGCGGTTTATCTGGCCGACTGCCGCAAGCTCGGCATCACCGTGCTCCCGCCCGACGTCAACGAATCTGGCTTGAACTTCGCATCGGTCGGCCAAGACATCCGCTACGGGCTGGGCGCGGTGCGCAACGTTGGCGCTAATGTCGTGGGCTCGTTGCTCCAAACCCGCAACGACAAGGGCAAGTTCACCGACTTTTCGGACTACCTGAACAAGATCGACATCTCGGCGTGCAACAAGAAGGTGACCGAATCGCTGATCAAGGCGGGTGCGTTCGACTCGCTGGGGCATGCCCGCAAGGGTCTTTTCCTGGTGCACAGCGATGCGGTGGACTCGGTGCTGGGCACCAAGAAGGCCGAGGCACTGGGGCAGTTCGATCTCTTCGGCAGCAATGATGATGGGACCGGCACCGCAGATCCCGTGTTCACCATCAAGGTGCCCGATGATGAGTGGGAGGACAAACACAAACTCGCCCTAGAGCGCGAGATGCTGGGACTGTACGTCTCGGGGCATCCCCTCAACGGTGTGGCACACTTGCTGGCTGCCCAGGTCGACACCGCGATCCCAGCGATCCTCGACGGCGATGTCCCCAACGATGCCCAAGTGCGGGTGGGCGGCATCCTGGCGTCGGTGAACCGGAGGGTCAACAAAAACGGAATGCCATGGGCTTCAGCGCAATTGGAGGATCTCACGGGCGGCATCGAGGTGATGTTCTTCCCGCACACCTACTCCAGCTATGGTGCCGACATCGTCGACGATGCCGTCGTGCTGGTCAACGCCAAGGTGGCGGTCCGTGACGACCGCATCGCATTGATCGCCAATGACCTCACAGTGCCCGACTTTTCCAACGCCGAGGTGGAGCGGCCGCTGGCGGTCAGCTTGCCCACCCGGCAGTGCACCTTTGACAAGGTGAGTGCGCTCAAACAGGTGTTGGCGCGCCACCCCGGCACCTCGCAGGTGCATCTGCGGCTCATCAGCGGAGACCGGATCACCACGCTGGCACTTGATCAGTCGTTGCGGGTGACGCCGTCGCCGGCGTTGATGGGTGACCTCAAGGAGCTGCTCGGCCCTGGATGTCTGGGGAGTTAG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity). {ECO:0000250}.

Family

DNA polymerase type-C family, DnaE subfamily

GO

DNA-directed DNA polymerase activity GO:0003887 molecular_function
cell wall GO:0005618 cellular_component
3'-5' exonuclease activity GO:0008408 molecular_function
DNA replication GO:0006260 biological_process
plasma membrane GO:0005886 cellular_component
cytoplasm GO:0005737 cellular_component

InterPro

UniProt: P9WNT7
GenBank: Rv1547
EnsemblBacteria: Rv1547
Mycobrowser: Rv1547

Summary

Name: DNA polymerase III subunit alpha (EC 2.7.7.7)
Family: DNA polymerase type-C family, DnaE subfamily
Protein Sequence: MSGSSAGSSFVHLHNHTEYSMLDGAAKITPMLAEVERLGMPAVGMTDHGNMFGASEFYNSATKAGIKPIIGVEAYIAPGSRFDTRRILWGDPSQKADDVSGSGSYTHLTMMAENATGLRNLFKLSSHASFEGQLSKWSRMDAELIAEHAEGIIITTGCPSGEVQTRLRLGQDREALEAAAKWREIVGPDNYFLELMDHGLTIERRVRDGLLEIGRALNIPPLATNDCHYVTRDAAHNHEALLCVQTGKTLSDPNRFKFDGDGYYLKSAAEMRQIWDDEVPGACDSTLLIAERVQSYADVWTPRDRMPVFPVPDGHDQASWLRHEVDAGLRRRFPAGPPDGYRERAAYEIDVICSKGFPSYFLIVADLISYARSAGIRVGPGRGSAAGSLVAYALGITDIDPIPHGLLFERFLNPERTSMPDIDIDFDDRRRGEMVRYAADKWGHDRVAQVITFGTIKTKAALKDSARIHYGQPGFAIADRITKALPPAIMAKDIPLSGITDPSHERYKEAAEVRGLIETDPDVRTIYQTARGLEGLIRNAGVHACAVIMSSEPLTEAIPLWKRPQDGAIITGWDYPACEAIGLLKMDFLGLRNLTIIGDAIDNVRANRGIDLDLESVPLDDKATYELLGRGDTLGVFQLDGGPMRDLLRRMQPTGFEDVVAVIALYRPGPMGMNAHNDYADRKNNRQAIKPIHPELEEPLREILAETYGLIVYQEQIMRIAQKVASYSLARADILRKAMGKKKREVLEKEFEGFSDGMQANGFSPAAIKALWDTILPFADYAFNKSHAAGYGMVSYWTAYLKANYPAEYMAGLLTSVGDDKDKAAVYLADCRKLGITVLPPDVNESGLNFASVGQDIRYGLGAVRNVGANVVGSLLQTRNDKGKFTDFSDYLNKIDISACNKKVTESLIKAGAFDSLGHARKGLFLVHSDAVDSVLGTKKAEALGQFDLFGSNDDGTGTADPVFTIKVPDDEWEDKHKLALEREMLGLYVSGHPLNGVAHLLAAQVDTAIPAILDGDVPNDAQVRVGGILASVNRRVNKNGMPWASAQLEDLTGGIEVMFFPHTYSSYGADIVDDAVVLVNAKVAVRDDRIALIANDLTVPDFSNAEVERPLAVSLPTRQCTFDKVSALKQVLARHPGTSQVHLRLISGDRITTLALDQSLRVTPSPALMGDLKELLGPGCLGS
Mass: 129,323 Da
Length: 1184 Aa

Rv1547 doesn't seem to be a targeted by any drug.

Homologous recombination mtu03440
mtu03440
Genetic Information Processing; Replication and repair

Mismatch repair mtu03430
mtu03430
The Escherichia coli MMR pathway has been extensively studied and is well characterized. In E. coli, the mismatch-activated MutS-MutL-ATP complex licenses MutH to incise the nearest unmethylated GATC sequence. UvrD and an exonuclease generate a gap. This gap is filled by pol III and DNA ligase. The GATC sites are then methylated by Dam. Several human MMR proteins have been identified based on their homology to E. coli MMR proteins. These include human homologs of MutS and MutL. Although E. coli MutS and MutL proteins are homodimers, human MutS and MutL homologs are heterodimers. The role of hemimethylated dGATC sites as a signal for strand discrimination is not conserved from E. coli to human. Human MMR is presumed to be nick-directed in vivo, and is thought to discriminate daughter and template strands using a strand-specific nick.

DNA replication mtu03030
mtu03030
Genetic Information Processing; Replication and repair

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.