Gene - combatTB X

ilvA

Type: protein_coding
Name: ilvA
Locus Name: Rv1559
Product: Probable threonine dehydratase IlvA
Functional Category: Intermediary metabolism and respiration
Location: 1763428..1764717 (+ strand)
Gene Length: 1289 bp
Nucleotides: TGTCCGCCGAACTGAGCCAGAGCCCGAGCAGCTCGCCGCTGTTTTCACTATCTGGGGCAGACATCGACCGTGCCGCCAAGCGGATCGCACCGGTAGTCACGCCCACCCCGTTGCAACCTAGCGATCGGTTGTCGGCGATCACTGGCGCCACGGTCTACCTCAAGCGCGAAGACTTGCAGACGGTGCGCTCTTATAAGCTACGCGGAGCGTACAACCTGTTGGTGCAGTTGTCCGATGAGGAACTGGCCGCGGGCGTGGTGTGTTCTTCTGCGGGCAACCACGCGCAGGGCTTCGCGTATGCGTGTCGCTGTCTGGGTGTGCACGGCCGGGTCTACGTACCTGCCAAAACCCCCAAGCAGAAGCGTGACCGGATCCGCTACCACGGCGGGGAGTTCATCGACCTGATCGTGGGTGGGTCGACCTATGATCTGGCTGCGGCGGCGGCCCTTGAGGACGTGGAACGCACCGGGGCCACGCTGGTACCGCCGTTTGACGACCTGCGCACCATCGCCGGCCAGGGCACGATAGCCGTCGAAGTGCTTGGCCAGCTCGAGGACGAGCCGGACCTGGTGGTGGTCCCGGTGGGTGGCGGCGGCTGCATCGCGGGGATCACCACCTACCTGGCCGAGCGGACGACCAACACCGCGGTGCTGGGCGTCGAGCCGGCTGGTGCGGCCGCCATGATGGCCGCGCTCGCGGCGGGCGAGCCGGTGACGCTGGACCATGTCGACCAGTTCGTCGACGGCGCCGCGGTGAACCGGGCGGGCACGCTGACCTATGCCGCGCTAGCCGCCGCCGGCGACATGGTTTCGCTCACCACCGTCGACGAGGGTGCGGTGTGCACGGCGATGCTCGATCTGTATCAGAACGAGGGCATCATCGCCGAACCGGCCGGTGCCCTGTCGGTCGCCGGTCTGTTGGAAGCCGACATCGAGCCCGGGTCCACCGTGGTGTGCCTGATTTCGGGCGGCAACAACGACGTGTCCCGTTACGGGGAGGTGTTGGAGCGCTCGCTGGTCCACCTGGGCCTCAAGCACTATTTCCTGGTCGACTTCCCGCAGGAGCCCGGTGCGCTGCGCCGGTTTCTCGACGACGTGCTCGGACCCAACGACGACATCACCTTGTTCGAGTACGTCAAGCGCAACAACCGGGAGACCGGTGAGGCGCTGGTGGGTATCGAGCTGGGATCGGCCGCGGATCTAGACGGTCTGCTGGCCCGGATGCGGGCGACCGACATTCACGTCGAGGCGTTGGAACCGGGGTCGCCGGCTTACCGCTATCTGCTGTAG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity). {ECO:0000250}.

Family

Serine/threonine dehydratase family

GO

isoleucine biosynthetic process GO:0009097 biological_process
plasma membrane GO:0005886 cellular_component
pyridoxal phosphate binding GO:0030170 molecular_function
threonine metabolic process GO:0006566 biological_process
threonine catabolic process GO:0006567 biological_process
L-threonine ammonia-lyase activity GO:0004794 molecular_function

InterPro

UniProt: P9WG95
GenBank: Rv1559
EnsemblBacteria: Rv1559
Mycobrowser: Rv1559

Summary

Name: L-threonine dehydratase biosynthetic IlvA (EC 4.3.1.19) (Threonine deaminase)
Family: Serine/threonine dehydratase family
Protein Sequence: MSAELSQSPSSSPLFSLSGADIDRAAKRIAPVVTPTPLQPSDRLSAITGATVYLKREDLQTVRSYKLRGAYNLLVQLSDEELAAGVVCSSAGNHAQGFAYACRCLGVHGRVYVPAKTPKQKRDRIRYHGGEFIDLIVGGSTYDLAAAAALEDVERTGATLVPPFDDLRTIAGQGTIAVEVLGQLEDEPDLVVVPVGGGGCIAGITTYLAERTTNTAVLGVEPAGAAAMMAALAAGEPVTLDHVDQFVDGAAVNRAGTLTYAALAAAGDMVSLTTVDEGAVCTAMLDLYQNEGIIAEPAGALSVAGLLEADIEPGSTVVCLISGGNNDVSRYGEVLERSLVHLGLKHYFLVDFPQEPGALRRFLDDVLGPNDDITLFEYVKRNNRETGEALVGIELGSAADLDGLLARMRATDIHVEALEPGSPAYRYLL
Mass: 45,041 Da
Length: 429 Aa

Rv1559 doesn't seem to be a targeted by any drug.

Glycine, serine and threonine metabolism mtu00260
mtu00260
Metabolism; Amino acid metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.