Gene - combatTB X

polA

Type: protein_coding
Name: polA
Locus Name: Rv1629
Product: Probable DNA polymerase I PolA
Functional Category: Information pathways
Location: 1830665..1833379 (+ strand)
Gene Length: 2714 bp
Nucleotides: TGGTGACTACTGCCAGTGCCCCCAGCGAGGATCGAGCCAAGCCGACGCTGATGTTGCTGGATGGCAATTCGCTGGCGTTTCGGGCGTTCTACGCACTGCCCGCGGAGAACTTCAAGACCCGCGGCGGGCTGACCACCAACGCCGTCTACGGCTTCACCGCCATGCTGATCAACCTGCTGCGCGATGAAGCCCCGACGCACATCGCGGCGGCTTTCGACGTGTCCCGGCAGACCTTCCGCTTGCAACGCTACCCGGAGTACAAGGCCAACCGATCGTCGACCCCCGACGAGTTCGCTGGCCAGATCGACATCACCAAAGAAGTGCTGGGCGCACTCGGCATCACCGTGCTCTCCGAGCCGGGGTTCGAGGCCGACGACCTCATCGCCACGCTGGCCACCCAGGCCGAGAACGAGGGCTACCGGGTGCTGGTGGTCACCGGGGATCGTGACGCACTGCAACTGGTCAGTGACGATGTGACGGTGCTCTACCCCCGCAAGGGCGTCAGCGAACTTACGCGCTTCACACCGGAGGCCGTCGTCGAAAAGTACGGGCTCACCCCTAGGCAGTACCCGGACTTCGCCGCGCTGCGCGGCGACCCCAGCGATAACCTGCCCGGCATACCCGGGGTGGGGGAGAAGACCGCCGCCAAATGGATCGCCGAGTACGGCTCGCTGCGGTCACTGGTGGACAACGTTGACGCCGTGCGCGGCAAGGTGGGCGATGCGCTGCGGGCGAACCTGGCCAGCGTGGTGCGCAACCGTGAGCTCACCGACCTGGTTCGCGACGTGCCGCTGGCCCAGACCCCGGACACGCTGCGGCTGCAGCCCTGGGATCGCGACCACATTCACCGGCTCTTCGACGACCTGGAGTTTCGGGTGTTGCGCGACCGGTTGTTCGACACGTTGGCCGCGGCCGGGGGACCCGAGGTCGACGAGGGGTTCGACGTGCGCGGCGGCGCGTTGGCGCCCGGCACGGTTAGGCAATGGTTGGCCGAGCACGCCGGCGACGGGCGCCGAGCGGGCCTGACGGTGGTGGGTACCCATCTGCCGCACGGTGGGGACGCTACCGCTATGGCCGTCGCCGCCGCCGACGGCGAAGGCGCTTACCTCGATACCGCGACGCTGACGCCCGACGACGACGCCGCGTTGGCGGCCTGGCTAGCGGATCCAGCTAAACCCAAAGCCTTGCATGAGGCAAAGGCGGCCGTTCATGACCTGGCGGGTCGTGGTTGGACCTTGGAGGGCGTCACCTCCGACACCGCACTGGCGGCCTACCTGGTGCGGCCGGGGCAGCGCAGCTTCACCCTCGACGACCTCTCGCTGCGCTATCTGCGTCGCGAGCTGCGTGCGGAAACACCGCAGCAGCAACAACTTTCACTGCTCGATGACGACGATACGGACGCCGAGACCATTCAAACGACGATCCTGCGGGCGCGGGCAGTCATCGACCTGGCCGACGCGCTGGACGCCGAGTTAGCGCGTATCGACTCCACCGCGCTGCTGGGGGAGATGGAGCTGCCGGTCCAGCGGGTGCTGGCGAAGATGGAAAGTGCCGGTATCGCCGTCGACCTGCCCATGTTGACCGAGCTGCAAAGCCAGTTTGGCGACCAGATCCGCGACGCCGCCGAGGCCGCCTACGGCGTGATCGGCAAGCAAATCAACCTGGGCTCACCCAAGCAGCTGCAGGTCGTGCTGTTCGACGAACTGGGCATGCCGAAGACCAAACGCACCAAGACCGGCTACACCACGGATGCCGACGCGCTGCAGTCGTTGTTCGACAAGACCGGGCATCCGTTTCTGCAACATCTGCTCGCCCACCGCGACGTCACCCGGCTCAAGGTCACCGTCGACGGGTTGCTCCAAGCGGTGGCCGCCGACGGCCGCATCCACACCACGTTCAACCAGACGATCGCCGCGACCGGCCGGCTCTCCTCGACCGAACCCAACCTGCAGAACATCCCGATCCGCACCGACGCGGGCCGGCGGATCCGGGACGCGTTCGTGGTCGGGGACGGCTACGCCGAGTTGATGACGGCCGACTACAGCCAGATCGAGATGCGGATCATGGCGCACCTGTCCGGGGACGAGGGCCTCATCGAGGCGTTCAACACCGGGGAGGACCTGCATTCGTTCGTCGCGTCCCGGGCGTTCGGCGTGCCCATCGACGAGGTCACCGGCGAGCTGCGGCGCCGGGTCAAGGCGATGTCCTACGGGCTGGCTTACGGGTTGAGCGCCTACGGCCTGTCGCAGCAGTTGAAAATCTCCACCGAGGAAGCCAACGAGCAGATGGACGCGTATTTCGCCCGATTCGGCGGGGTGCGCGACTACCTGCGCGCCGTAGTCGAGCGGGCCCGCAAGGACGGCTACACCTCGACGGTGCTGGGCCGTCGCCGCTACCTGCCCGAGCTGGACAGCAGCAACCGTCAAGTGCGGGAGGCCGCCGAGCGGGCGGCGCTGAACGCGCCGATCCAGGGCAGCGCGGCCGACATCATCAAGGTGGCCATGATCCAGGTCGACAAGGCGCTCAACGAGGCACAGCTGGCGTCGCGCATGCTGCTGCAGGTCCACGACGAGCTGCTGTTCGAAATCGCCCCCGGTGAACGCGAGCGGGTCGAGGCCCTGGTGCGCGACAAGATGGGCGGCGCTTACCCGCTCGACGTCCCGCTGGAGGTGTCGGTGGGCTACGGCCGCAGCTGGGACGCGGCGGCGCACTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.

Family

DNA polymerase type-A family

GO

DNA-directed DNA polymerase activity GO:0003887 molecular_function
cell wall GO:0005618 cellular_component
3'-5' exonuclease activity GO:0008408 molecular_function
5'-3' exonuclease activity GO:0008409 molecular_function
DNA binding GO:0003677 molecular_function
DNA-dependent DNA replication GO:0006261 biological_process
DNA repair GO:0006281 biological_process
plasma membrane GO:0005886 cellular_component
cytoplasm GO:0005737 cellular_component

InterPro

UniProt: P9WNU5
GenBank: Rv1629
EnsemblBacteria: Rv1629
Mycobrowser: Rv1629

Summary

Name: DNA polymerase I (POL I) (EC 2.7.7.7)
Family: DNA polymerase type-A family
Protein Sequence: MVTTASAPSEDRAKPTLMLLDGNSLAFRAFYALPAENFKTRGGLTTNAVYGFTAMLINLLRDEAPTHIAAAFDVSRQTFRLQRYPEYKANRSSTPDEFAGQIDITKEVLGALGITVLSEPGFEADDLIATLATQAENEGYRVLVVTGDRDALQLVSDDVTVLYPRKGVSELTRFTPEAVVEKYGLTPRQYPDFAALRGDPSDNLPGIPGVGEKTAAKWIAEYGSLRSLVDNVDAVRGKVGDALRANLASVVRNRELTDLVRDVPLAQTPDTLRLQPWDRDHIHRLFDDLEFRVLRDRLFDTLAAAGGPEVDEGFDVRGGALAPGTVRQWLAEHAGDGRRAGLTVVGTHLPHGGDATAMAVAAADGEGAYLDTATLTPDDDAALAAWLADPAKPKALHEAKAAVHDLAGRGWTLEGVTSDTALAAYLVRPGQRSFTLDDLSLRYLRRELRAETPQQQQLSLLDDDDTDAETIQTTILRARAVIDLADALDAELARIDSTALLGEMELPVQRVLAKMESAGIAVDLPMLTELQSQFGDQIRDAAEAAYGVIGKQINLGSPKQLQVVLFDELGMPKTKRTKTGYTTDADALQSLFDKTGHPFLQHLLAHRDVTRLKVTVDGLLQAVAADGRIHTTFNQTIAATGRLSSTEPNLQNIPIRTDAGRRIRDAFVVGDGYAELMTADYSQIEMRIMAHLSGDEGLIEAFNTGEDLHSFVASRAFGVPIDEVTGELRRRVKAMSYGLAYGLSAYGLSQQLKISTEEANEQMDAYFARFGGVRDYLRAVVERARKDGYTSTVLGRRRYLPELDSSNRQVREAAERAALNAPIQGSAADIIKVAMIQVDKALNEAQLASRMLLQVHDELLFEIAPGERERVEALVRDKMGGAYPLDVPLEVSVGYGRSWDAAAH
Mass: 98,472 Da
Length: 904 Aa

Rv1629 doesn't seem to be a targeted by any drug.

Homologous recombination mtu03440
mtu03440
Genetic Information Processing; Replication and repair

Nucleotide excision repair mtu03420
mtu03420
Genetic Information Processing; Replication and repair

Base excision repair mtu03410
mtu03410
Genetic Information Processing; Replication and repair

DNA replication mtu03030
mtu03030
Genetic Information Processing; Replication and repair

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
A PCR method for the sequence analysis of the gyrA, polA and rnhA gene segments from mycobacteria.: Gene. 1993 Dec 22;136(1-2):287-90.