Gene - combatTB X

uvrB

Type: protein_coding
Name: uvrB
Locus Name: Rv1633
Product: Probable excinuclease ABC (subunit B-helicase) UvrB
Functional Category: Information pathways
Location: 1837075..1839171 (+ strand)
Gene Length: 2096 bp
Nucleotides: TGCGCGCCGGCGGTCACTTCGAGGTGGTCAGTCCGCATGCTCCGGCCGGCGACCAGCCGGCCGCAATCGACGAGCTGGAGCGGCGGATCAACGCGGGGGAGCGTGACGTGGTGTTGCTCGGCGCCACCGGCACCGGGAAGTCGGCGACCACCGCGTGGCTGATCGAACGCCTGCAGCGGCCCACCCTGGTGATGGCGCCCAACAAGACGTTGGCCGCCCAGCTGGCGAACGAACTGCGAGAGATGTTGCCGCACAACGCCGTCGAGTACTTCGTCTCGTACTACGACTACTACCAGCCGGAGGCGTATATCGCGCAGACCGACACTTATATCGAAAAGGATAGCTCCATCAACGACGACGTGGAGCGGCTGCGGCACTCCGCGACCTCGGCGCTGCTGTCGCGTCGTGACGTGGTGGTGGTGGCTTCGGTGTCCTGCATCTACGGCCTGGGCACACCGCAGTCCTACCTGGACCGCTCCGTCGAGCTGAAGGTGGGCGAGGAAGTGCCGCGCGATGGGCTGCTGCGGCTGCTGGTCGACGTGCAATACACCCGAAACGACATGTCCTTTACTCGCGGCTCGTTTCGGGTGCGCGGCGACACCGTCGAGATCATCCCCTCCTACGAAGAGCTGGCGGTTCGCATCGAGTTCTTCGGCGACGAGATCGAGGCGCTGTACTATCTGCACCCGCTGACCGGCGAGGTTATCCGCCAGGTCGACTCGCTGCGGATCTTTCCCGCTACCCATTACGTCGCCGGTCCGGAGCGGATGGCGCATGCCGTCTCGGCCATCGAGGAAGAACTCGCCGAGCGACTCGCCGAGCTTGAGAGCCAGGGCAAGCTGCTGGAGGCGCAGCGGCTGCGGATGCGCACCAACTACGACATCGAAATGATGCGGCAGGTCGGGTTCTGCTCGGGCATCGAGAACTACTCCCGCCACATCGACGGTAGGGGGCCCGGCACGCCGCCCGCGACCCTGCTCGACTATTTCCCCGAGGATTTCCTGCTCGTTATCGACGAGTCACATGTCACCGTGCCGCAGATCGGCGGCATGTACGAGGGCGACATCTCCCGCAAGCGCAACCTGGTGGAGTACGGTTTCCGGCTGCCGTCGGCGTGCGACAACCGTCCGCTGACCTGGGAGGAGTTCGCTGACCGGATCGGGCAGACGGTGTATCTGTCTGCCACCCCGGGGCCCTACGAGCTCAGCCAGACCGGCGGCGAGTTCGTCGAGCAGGTGATCCGGCCGACCGGTCTGGTGGACCCGAAAGTGGTAGTCAAGCCGACCAAAGGGCAGATCGACGACCTGATCGGCGAGATCCGCACACGGGCAGACGCCGACCAGCGGGTGCTGGTGACGACGCTGACCAAGAAGATGGCCGAAGACCTCACCGACTACCTGCTGGAGATGGGCATTCGGGTGCGCTACCTGCATTCGGAGGTCGACACGTTGCGCCGGGTCGAGTTGTTGCGCCAGCTGCGTCTGGGTGACTACGACGTGCTGGTCGGCATCAACCTGCTCCGCGAGGGCCTAGACCTGCCCGAGGTGTCGCTGGTGGCGATCCTCGACGCCGACAAAGAAGGATTCCTGCGGTCAAGCCGCAGCCTGATCCAGACCATCGGACGCGCCGCTCGCAACGTGTCCGGCGAGGTGCACATGTACGCCGACAAAATCACCGACTCGATGAGGGAAGCCATCGACGAGACCGAACGCCGGCGGGCCAAGCAGATCGCCTACAACGAGGCCAACGGAATCGACCCACAGCCGCTGCGCAAAAAGATCGCCGACATCCTCGATCAGGTCTATCGGGAGGCCGACGACACCGCCGTCGTCGAGGTCGGCGGATCCGGGCGCAACGCATCCCGCGGCCGGCGGGCTCAGGGTGAGCCCGGCCGGGCGGTCAGCGCCGGCGTGTTCGAGGGCCGCGACACCTCCGCCATGCCGCGCGCTGAGCTGGCCGACCTAATCAAAGACCTCACCGCACAGATGATGGCGGCCGCGCGCGACCTGCAGTTCGAGCTGGCGGCCCGGTTCCGCGACGAGATCGCCGACCTCAAGCGGGAGCTGCGGGGGATGGACGCGGCCGGCCTGAAGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage. {ECO:0000255|HAMAP-Rule:MF_00204}.

Family

UvrB family

GO

SOS response GO:0009432 biological_process
excinuclease repair complex GO:0009380 cellular_component
excinuclease ABC activity GO:0009381 molecular_function
ATP binding GO:0005524 molecular_function
DNA binding GO:0003677 molecular_function
positive regulation by symbiont of host immune response GO:0052556 biological_process
nucleotide-excision repair GO:0006289 biological_process
plasma membrane GO:0005886 cellular_component
cytoplasm GO:0005737 cellular_component
response to nitrosative stress GO:0051409 biological_process
ATPase activity GO:0016887 molecular_function

InterPro

UniProt: P9WFC7
GenBank: Rv1633
EnsemblBacteria: Rv1633
Mycobrowser: Rv1633

Summary

Name: UvrABC system protein B (Protein UvrB) (Excinuclease ABC subunit B)
Family: UvrB family
Protein Sequence: MAFATEHPVVAHSEYRAVEEIVRAGGHFEVVSPHAPAGDQPAAIDELERRINAGERDVVLLGATGTGKSATTAWLIERLQRPTLVMAPNKTLAAQLANELREMLPHNAVEYFVSYYDYYQPEAYIAQTDTYIEKDSSINDDVERLRHSATSALLSRRDVVVVASVSCIYGLGTPQSYLDRSVELKVGEEVPRDGLLRLLVDVQYTRNDMSFTRGSFRVRGDTVEIIPSYEELAVRIEFFGDEIEALYYLHPLTGEVIRQVDSLRIFPATHYVAGPERMAHAVSAIEEELAERLAELESQGKLLEAQRLRMRTNYDIEMMRQVGFCSGIENYSRHIDGRGPGTPPATLLDYFPEDFLLVIDESHVTVPQIGGMYEGDISRKRNLVEYGFRLPSACDNRPLTWEEFADRIGQTVYLSATPGPYELSQTGGEFVEQVIRPTGLVDPKVVVKPTKGQIDDLIGEIRTRADADQRVLVTTLTKKMAEDLTDYLLEMGIRVRYLHSEVDTLRRVELLRQLRLGDYDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSSRSLIQTIGRAARNVSGEVHMYADKITDSMREAIDETERRRAKQIAYNEANGIDPQPLRKKIADILDQVYREADDTAVVEVGGSGRNASRGRRAQGEPGRAVSAGVFEGRDTSAMPRAELADLIKDLTAQMMAAARDLQFELAARFRDEIADLKRELRGMDAAGLK
Mass: 80,407 Da
Length: 719 Aa

Rv1633 doesn't seem to be a targeted by any drug.

Nucleotide excision repair mtu03420
mtu03420
Genetic Information Processing; Replication and repair

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.