Gene - combatTB X

uvrA

Type: protein_coding
Name: uvrA
Locus Name: Rv1638
Product: Probable excinuclease ABC (subunit A-DNA-binding ATPase) UvrA
Functional Category: Information pathways
Location: 1843741..1846659 (+ strand)
Gene Length: 2918 bp
Nucleotides: TGGCTGACCGCCTGATCGTCAAGGGTGCGCGCGAACACAATCTGCGCAGCGTCGACCTCGACCTGCCCCGCGACGCGCTGATCGTCTTCACCGGGTTATCCGGATCGGGCAAGTCCTCGCTCGCGTTCGACACCATCTTCGCCGAGGGGCAGCGGCGTTACGTGGAGTCGCTGTCGGCCTACGCCCGCCAATTTCTCGGGCAGATGGACAAGCCGGACGTCGACTTCATCGAGGGGCTGTCTCCGGCGGTGTCCATCGACCAGAAGTCGACCAACCGCAACCCACGATCGACGGTCGGGACCATCACCGAGGTGTACGACTACCTGCGGCTGTTGTATGCGCGCGCGGGCACGCCGCACTGCCCGACCTGCGGGGAGCGAGTCGCGCGCCAAACCCCGCAACAAATCGTCGATCAGGTGCTGGCCATGCCGGAGGGCACTCGGTTTCTGGTGCTGGCCCCGGTGGTGCGTACCCGCAAGGGCGAGTTCGCCGATCTGTTCGATAAGCTCAACGCCCAGGGCTACAGCCGGGTGCGGGTCGACGGTGTGGTGCATCCGCTGACCGATCCGCCGAAGCTGAAAAAGCAGGAAAAGCACGACATCGAGGTGGTGGTGGACCGTCTCACCGTCAAGGCCGCCGCCAAGCGGCGGCTCACCGATTCGGTGGAAACCGCGCTGAATTTGGCCGACGGGATCGTGGTGCTCGAATTCGTCGATCATGAACTGGGTGCACCGCATCGCGAGCAGCGGTTCTCCGAGAAGCTGGCCTGCCCCAACGGGCACGCGCTGGCCGTCGACGACCTGGAGCCGCGGTCGTTCTCGTTCAACTCGCCCTACGGCGCCTGCCCCGAATGCAGTGGTCTGGGCATCCGCAAGGAGGTCGACCCGGAGCTGGTGGTGCCCGATCCGGATCGCACCCTGGCGCAGGGTGCGGTGGCGCCGTGGTCGAACGGCCACACCGCGGAGTACTTCACCCGGATGATGGCCGGCCTTGGCGAGGCGCTCGGGTTCGACGTCGACACGCCCTGGCGCAAGCTGCCGGCCAAGGCCCGCAAGGCGATTCTGGAAGGCGCCGACGAGCAGGTGCACGTGCGCTACCGCAACCGCTACGGACGCACCCGGTCGTATTACGCCGATTTCGAGGGTGTGCTGGCGTTCCTGCAACGCAAGATGTCCCAAACCGAGTCCGAGCAGATGAAGGAGCGCTACGAGGGTTTCATGCGGGACGTGCCCTGCCCGGTGTGTGCGGGCACCCGGCTCAAGCCCGAGATTCTGGCGGTGACGCTGGCTGGGGAGTCCAAGGGGGAGCACGGCGCCAAGTCCATCGCCGAGGTGTGTGAGCTGTCGATCGCCGACTGCGCGGACTTCCTGAACGCGCTCACGCTGGGTCCGCGCGAGCAAGCGATCGCCGGGCAGGTGCTCAAGGAGATCCGGTCGCGGCTCGGGTTTCTGCTCGACGTCGGGCTGGAGTACCTGTCGCTGTCCCGGGCGGCGGCCACGCTGTCCGGCGGTGAGGCACAACGTATCCGGCTGGCCACCCAGATCGGCTCCGGCCTGGTGGGTGTGCTCTACGTGCTCGACGAGCCGTCCATCGGGCTGCACCAGCGCGACAACCGTCGTCTTATCGAAACCCTCACCCGGTTACGGGATTTGGGGAACACTTTGATCGTCGTCGAGCACGACGAGGACACCATCGAGCATGCGGACTGGATCGTCGACATCGGCCCGGGGGCCGGTGAGCACGGTGGCCGCATCGTGCACAGCGGGCCCTACGATGAACTGCTACGCAACAAGGATTCGATCACCGGCGCCTACCTGTCCGGCCGGGAAAGCATTGAGATACCGGCGATTCGGCGTTCCGTCGACCCCCGTCGTCAACTCACCGTCGTCGGCGCCCGCGAGCACAACTTGCGCGGGATCGATGTGTCTTTCCCGCTGGGTGTGCTGACCTCGGTGACCGGTGTCTCGGGTTCGGGCAAGTCGACGTTGGTCAACGACATCCTGGCCGCGGTGCTGGCCAACCGCCTCAACGGCGCCCGGCAGGTCCCCGGCCGGCACACCCGGGTCACCGGGCTGGACTATCTGGACAAGCTGGTGCGGGTGGACCAATCGCCGATCGGGCGCACACCGCGATCCAACCCGGCCACCTACACCGGTGTGTTCGACAAGATCCGCACCCTGTTCGCCGCCACCACCGAGGCCAAGGTCCGCGGCTATCAACCCGGACGATTCTCGTTCAACGTCAAGGGCGGTCGCTGCGAGGCCTGCACCGGCGACGGCACCATCAAGATCGAGATGAACTTCCTGCCCGACGTGTACGTGCCGTGCGAGGTCTGCCAGGGGGCCCGGTACAACCGCGAAACCCTCGAGGTGCACTACAAGGGCAAGACCGTCTCGGAAGTGCTGGACATGTCCATCGAGGAAGCGGCGGAGTTCTTCGAGCCGATCGCCGGCGTCCATCGCTATCTACGCACCCTGGTCGACGTGGGCCTGGGCTACGTGCGGCTCGGCCAGCCCGCGCCCACGCTGTCCGGCGGTGAGGCCCAGCGGGTCAAGCTGGCCTCGGAGCTGCAGAAGCGCTCCACCGGGCGCACCGTCTACATCCTCGACGAGCCGACGACGGGACTGCACTTCGACGACATACGCAAGCTGCTCAACGTGATCAACGGCCTGGTCGACAAGGGCAATACGGTGATCGTCATCGAACATAACCTGGACGTGATCAAGACATCGGATTGGATCATCGACCTGGGCCCGGAGGGCGGTGCCGGCGGCGGAACCGTTGTCGCCCAAGGCACTCCGGAGGACGTTGCCGCGGTGCCGGCGAGCTACACCGGGAAGTTTCTCGCTGAGGTCGTCGGCGGCGGTGCCTCGGCCGCCACATCGCGGTCGAACAGACGGCGCAACGTCAGCGCCTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate. Alone it slightly inhibits RecA-mediated DNA strand exchange, in concert with UvrD1 greatly inhibits RecA-mediated DNA strand exchange. {ECO:0000255|HAMAP-Rule:MF_00205, ECO:0000269|PubMed:20455546, ECO:0000269|PubMed:22467787}.

Family

ABC transporter superfamily, UvrA family

GO

SOS response GO:0009432 biological_process
excinuclease repair complex GO:0009380 cellular_component
excinuclease ABC activity GO:0009381 molecular_function
cell wall GO:0005618 cellular_component
ATP binding GO:0005524 molecular_function
cytosol GO:0005829 cellular_component
DNA binding GO:0003677 molecular_function
nucleotide-excision repair GO:0006289 biological_process
plasma membrane GO:0005886 cellular_component
negative regulation of strand invasion GO:0060543 biological_process
zinc ion binding GO:0008270 molecular_function
ATPase activity GO:0016887 molecular_function
cellular response to DNA damage stimulus GO:0006974 biological_process

InterPro

UniProt: P9WQK7
GenBank: Rv1638
EnsemblBacteria: Rv1638
Mycobrowser: Rv1638

Summary

Name: UvrABC system protein A (UvrA protein) (Excinuclease ABC subunit A)
Family: ABC transporter superfamily, UvrA family
Protein Sequence: MADRLIVKGAREHNLRSVDLDLPRDALIVFTGLSGSGKSSLAFDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTITEVYDYLRLLYARAGTPHCPTCGERVARQTPQQIVDQVLAMPEGTRFLVLAPVVRTRKGEFADLFDKLNAQGYSRVRVDGVVHPLTDPPKLKKQEKHDIEVVVDRLTVKAAAKRRLTDSVETALNLADGIVVLEFVDHELGAPHREQRFSEKLACPNGHALAVDDLEPRSFSFNSPYGACPECSGLGIRKEVDPELVVPDPDRTLAQGAVAPWSNGHTAEYFTRMMAGLGEALGFDVDTPWRKLPAKARKAILEGADEQVHVRYRNRYGRTRSYYADFEGVLAFLQRKMSQTESEQMKERYEGFMRDVPCPVCAGTRLKPEILAVTLAGESKGEHGAKSIAEVCELSIADCADFLNALTLGPREQAIAGQVLKEIRSRLGFLLDVGLEYLSLSRAAATLSGGEAQRIRLATQIGSGLVGVLYVLDEPSIGLHQRDNRRLIETLTRLRDLGNTLIVVEHDEDTIEHADWIVDIGPGAGEHGGRIVHSGPYDELLRNKDSITGAYLSGRESIEIPAIRRSVDPRRQLTVVGAREHNLRGIDVSFPLGVLTSVTGVSGSGKSTLVNDILAAVLANRLNGARQVPGRHTRVTGLDYLDKLVRVDQSPIGRTPRSNPATYTGVFDKIRTLFAATTEAKVRGYQPGRFSFNVKGGRCEACTGDGTIKIEMNFLPDVYVPCEVCQGARYNRETLEVHYKGKTVSEVLDMSIEEAAEFFEPIAGVHRYLRTLVDVGLGYVRLGQPAPTLSGGEAQRVKLASELQKRSTGRTVYILDEPTTGLHFDDIRKLLNVINGLVDKGNTVIVIEHNLDVIKTSDWIIDLGPEGGAGGGTVVAQGTPEDVAAVPASYTGKFLAEVVGGGASAATSRSNRRRNVSA
Mass: 106,132 Da
Length: 972 Aa

Rv1638 doesn't seem to be a targeted by any drug.

Nucleotide excision repair mtu03420
mtu03420
Genetic Information Processing; Replication and repair

Important role for Mycobacterium tuberculosis UvrD1 in pathogenesis and persistence apart from its function in nucleotide excision repair.: J Bacteriol. 2012 Jun;194(11):2916-23. doi: 10.1128/JB.06654-11. Epub 2012 Mar 30.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Mycobacterium tuberculosis UvrD1 and UvrA proteins suppress DNA strand exchange promoted by cognate and noncognate RecA proteins.: Biochemistry. 2010 Jun 15;49(23):4872-83. doi: 10.1021/bi902021d.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.