Gene - combatTB X

pknF

Type: protein_coding
Name: pknF
Locus Name: Rv1746
Product: Anchored-membrane serine/threonine-protein kinase PknF (protein kinase F) (STPK F)
Functional Category: Regulatory proteins
Location: 1972138..1973568 (+ strand)
Gene Length: 1430 bp
Nucleotides: TGCCGCTCGCGGAAGGTTCGACGTTCGCCGGCTTCACCATCGTCCGGCAGTTGGGATCCGGCGGGATGGGCGAGGTGTACCTGGCCCGGCATCCCAGACTGCCCCGCCAGGACGCGCTCAAGGTACTGCGGGCCGATGTGTCAGCCGACGGCGAATACCGGGCACGGTTCAACCGCGAAGCCGATGCCGCGGCGTCGCTGTGGCATCCACACATCGTCGCCGTCCACGACCGCGGCGAGTTCGACGGCCAGCTCTGGATCGACATGGACTTCGTCGACGGCACCGACACCGTATCCCTTCTCAGGGATCGTTATCCGAACGGGATGCCCGGCCCCGAGGTCACCGAGATCATCACTGCGGTGGCCGAAGCGCTCGACTATGCCCACGAACGTCGGCTGTTGCACCGCGACGTCAAACCCGCCAACATCCTGATCGCCAATCCTGATTCACCTGATCGTCGAATCATGTTGGCCGACTTCGGGATCGCCGGCTGGGTCGATGATCCAAGCGGATTGACCGCCACAAACATGACTGTGGGCACCGTGTCATACGCGGCTCCGGAACAGCTTATGGGCAACGAGCTCGATGGACGGGCCGACCAATACGCACTAGCCGCGACGGCGTTTCACTTGCTGACCGGCTCCCCGCCCTTTCAGCACGCCAACCCCGCCGTGGTGATCAGCCAGCATCTCAGCGCGTCACCCCCGGCGATCGGCGATCGGGTTCCCGAGCTGACACCGCTGGACCCGGTCTTCGCCAAAGCGCTGGCCAAGCAACCCAAGGACCGTTACCAGCGGTGTGTCGACTTCGCGCGCGCACTCGGCCATCGTCTGGGCGGCGCGGGTGATCCTGACGACACGCGGGTGTCGCAACCGGTCGCCGTGGCCGCGCCCGCGAAACGCTCGCTGCTGCGGACCGCCGTCATCGTCCCCGCGGTGCTGGCGATGCTGCTGGTGATGGCCGTCGCGGTCGCCGTGCGGGAGTTCCAGCGTGCTGACGACGAGCGTGCAGCGCAGCCTGCGCGGACGCGGACCACCACATCGGCCGGCACGACCACTTCGGTAGCCCCCGCGAGCACAACGCGCCCGGCCCCCACGACCCCGACCACGACTGGCGCCGCCGACACCGCGACTGCATCGCCGACCGCTGCGGTTGTCGCCATCGGCGCCCTCTGCTTCCCGCTCGGCAGCACCGGCACCACCAAGACCGGGGCGACGGCCTACTGCTCGACGCTGCAAGGCACCAACACCACCATCTGGTCGCTGACCGAGGACACCGTGGCCAGTCCGACTGTGACCGCCACTGCTGACCCGACGGAGGCGCCGCTGCCCATCGAGCAGGAATCGCCGATTCGAGTGTGCATGCAGCAGACCGGCCAGACCCGACGGGAATGTCGCGAGGAGATTCGCAGAAGCAACGGCTGGCCGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Phosphorylates the FHA domains of the ABC transporter Rv1747, the heat-shock protein GroEL 1, and Rv0020c. May play a role in the regulation of glucose transport, cell growth and septum formation. {ECO:0000269|PubMed:15135525, ECO:0000269|PubMed:15866927, ECO:0000269|PubMed:15987910, ECO:0000269|PubMed:19201798, ECO:0000269|PubMed:21622570}.

Family

Protein kinase superfamily, Ser/Thr protein kinase family

GO

protein kinase activity GO:0004672 molecular_function
protein serine/threonine kinase activity GO:0004674 molecular_function
negative regulation of lipid biosynthetic process GO:0051055 biological_process
regulation of cell growth GO:0001558 biological_process
integral component of plasma membrane GO:0005887 cellular_component
cytosol GO:0005829 cellular_component
ATP binding GO:0005524 molecular_function
negative regulation of catalytic activity GO:0043086 biological_process
positive regulation of catalytic activity GO:0043085 biological_process
septin ring assembly GO:0000921 biological_process
regulation of glucose transmembrane transport GO:0010827 biological_process

InterPro

UniProt: P9WI75
GenBank: Rv1746
EnsemblBacteria: Rv1746
Mycobrowser: Rv1746

Summary

Name: Serine/threonine-protein kinase PknF (EC 2.7.11.1)
Family: Protein kinase superfamily, Ser/Thr protein kinase family
Protein Sequence: MPLAEGSTFAGFTIVRQLGSGGMGEVYLARHPRLPRQDALKVLRADVSADGEYRARFNREADAAASLWHPHIVAVHDRGEFDGQLWIDMDFVDGTDTVSLLRDRYPNGMPGPEVTEIITAVAEALDYAHERRLLHRDVKPANILIANPDSPDRRIMLADFGIAGWVDDPSGLTATNMTVGTVSYAAPEQLMGNELDGRADQYALAATAFHLLTGSPPFQHANPAVVISQHLSASPPAIGDRVPELTPLDPVFAKALAKQPKDRYQRCVDFARALGHRLGGAGDPDDTRVSQPVAVAAPAKRSLLRTAVIVPAVLAMLLVMAVAVAVREFQRADDERAAQPARTRTTTSAGTTTSVAPASTTRPAPTTPTTTGAADTATASPTAAVVAIGALCFPLGSTGTTKTGATAYCSTLQGTNTTIWSLTEDTVASPTVTATADPTEAPLPIEQESPIRVCMQQTGQTRRECREEIRRSNGWP
Mass: 50,668 Da
Length: 476 Aa

Rv1746 doesn't seem to be a targeted by any drug.

Rv1746 doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Serine/threonine protein kinases PknF and PknG of Mycobacterium tuberculosis: characterization and localization.: Microbiology. 2001 Aug;147(Pt 8):2307-14. doi: 10.1099/00221287-147-8-2307.
Two FHA domains on an ABC transporter, Rv1747, mediate its phosphorylation by PknF, a Ser/Thr protein kinase from Mycobacterium tuberculosis.: FEMS Microbiol Lett. 2004 May 15;234(2):215-23. doi: 10.1016/j.femsle.2004.03.033.
Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases.: Biochem Biophys Res Commun. 2005 Aug 5;333(3):858-67. doi: 10.1016/j.bbrc.2005.05.173.
An ABC transporter containing a forkhead-associated domain interacts with a serine-threonine protein kinase and is required for growth of Mycobacterium tuberculosis in mice.: Infect Immun. 2005 Aug;73(8):4471-7. doi: 10.1128/IAI.73.8.4471-4477.2005.
Role of Mycobacterium tuberculosis Ser/Thr kinase PknF: implications in glucose transport and cell division.: J Bacteriol. 2005 May;187(10):3415-20. doi: 10.1128/JB.187.10.3415-3420.2005.
Mycobacterium tuberculosis serine/threonine kinases PknB, PknD, PknE, and PknF phosphorylate multiple FHA domains.: Protein Sci. 2005 Jul;14(7):1918-21. doi: 10.1110/ps.051413405.
The Mycobacterium tuberculosis GroEL1 chaperone is a substrate of Ser/Thr protein kinases.: J Bacteriol. 2009 Apr;191(8):2876-83. doi: 10.1128/JB.01569-08. Epub 2009 Feb 6.
Forkhead-associated (FHA) domain containing ABC transporter Rv1747 is positively regulated by Ser/Thr phosphorylation in Mycobacterium tuberculosis.: J Biol Chem. 2011 Jul 22;286(29):26198-209. doi: 10.1074/jbc.M111.246132. Epub 2011 May 26.