Gene - combatTB X

gcvB

Type: protein_coding
Name: gcvB
Locus Name: Rv1832
Product: Probable glycine dehydrogenase GcvB (glycine decarboxylase) (glycine cleavage system P-protein)
Functional Category: Intermediary metabolism and respiration
Location: 2075877..2078702 (+ strand)
Gene Length: 2825 bp
Nucleotides: TGTCCGACCATTCGACGTTCGCAGACCGGCACATCGGTCTGGACAGCCAGGCCGTCGCGACCATGCTCGCCGTGATCGGGGTGGATTCGCTCGATGACCTGGCAGTCAAGGCGGTCCCGGCGGGCATCCTAGACACACTCACCGACACCGGAGCCGCACCGGGTTTGGACAGTCTGCCACCGGCTGCCAGCGAAGCCGAGGCGCTGGCCGAGCTGCGAGCGCTGGCCGACGCTAACACCGTCGCCGTGTCGATGATCGGGCAAGGCTACTACGACACACACACCCCCCCGGTGCTGTTGCGCAACATCATCGAGAACCCGGCCTGGTATACCGCCTACACGCCGTACCAGCCCGAGATTAGTCAGGGTCGGCTGGAAGCCTTGCTGAACTTCCAGACCCTGGTCACCGATCTGACCGGCCTCGAGATCGCGAACGCGTCGATGCTCGACGAGGGCACCGCGGCGGCCGAGGCCATGACTTTGATGCACCGCGCGGCCCGCGGGCCGGTGAAGAGGGTGGTCGTGGACGCCGACGTGTTCACCCAGACCGCGGCGGTGCTGGCCACCCGCGCCAAGCCGCTGGGTATCGAGATCGTCACGGCCGACCTGCGCGCCGGTCTGCCCGACGGCGAATTTTTCGGCGTCATCGCCCAGCTGCCCGGGGCCAGCGGCCGGATCACCGACTGGTCTGCCCTGGTGCAACAGGCCCACGACCGTGGCGCACTGGTGGCCGTCGGCGCCGACTTGTTGGCGCTGACGCTGATCGCGCCGCCCGGAGAGATCGGCGCTGACGTCGCCTTTGGCACCACACAACGGTTCGGAGTGCCGATGGGGTTTGGCGGCCCGCATGCCGGGTACCTTGCGGTGCACGCCAAGCATGCGCGTCAGCTGCCCGGCCGGCTGGTCGGTGTGTCCGTCGACAGTGACGGCACGCCGGCCTATCGGTTGGCGCTGCAGACTCGCGAGCAACACATCCGCCGCGACAAGGCCACCAGCAACATCTGCACCGCACAAGTGCTGTTGGCGGTGCTTGCCGCGATGTACGCGAGCTACCACGGCGCGGGCGGGCTGACCGCCATCGCACGCCGGGTGCATGCCCACGCCGAGGCTATCGCCGGTGCACTGGGCGATGCGTTGGTGCACGACAAGTACTTCGACACGGTGTTGGCCCGGGTGCCCGGTCGTGCCGACGAGGTGCTGGCCAGGGCCAAGGCCAACGGCATCAACCTGTGGCGTGTCGACGCCGACCATGTGTCGGTAGCCTGCGACGAAGCCACCACTGACACCCACGTGGCGGTCGTTCTGGACGCGTTCGGTGTAGCGGCCGCCGCACCCGCCCATACGGACATCGCAACGCGCACATCGGAGTTCCTGACGCATCCAGCGTTCACGCAATACCGCACCGAGACGTCGATGATGCGGTACTTGCGTGCGCTGGCGGATAAGGATATTGCCCTCGACCGCAGCATGATTCCGCTCGGCTCGTGCACGATGAAACTCAACGCCGCCGCCGAGATGGAGTCGATTACCTGGCCTGAATTCGGGCGTCAGCATCCATTTGCCCCGGCATCTGATACCGCTGGGCTGCGTCAACTTGTTGCCGACCTACAGAGTTGGCTGGTGCTGATCACCGGTTATGACGCGGTGTCGCTGCAACCTAACGCGGGCTCGCAAGGCGAGTATGCGGGCCTATTGGCGATCCACGAGTACCACGCCAGCCGGGGTGAACCGCATCGCGACATCTGCCTGATCCCGTCCAGCGCGCACGGCACCAATGCCGCGTCAGCCGCCTTGGCCGGCATGCGCGTGGTGGTGGTGGACTGCCACGACAACGGCGACGTCGACCTCGATGACCTGCGCGCTAAGGTCGGGGAGCATGCCGAGCGGTTGTCGGCGCTAATGATCACCTACCCGTCCACTCACGGCGTGTACGAACACGACATCGCCGAGATCTGCGCTGCCGTGCACGACGCGGGCGGCCAGGTATACGTCGACGGAGCCAACCTCAACGCCCTGGTCGGCCTGGCCCGGCCGGGCAAGTTCGGCGGTGACGTCAGTCACCTCAACCTACACAAGACATTCTGCATTCCGCACGGCGGCGGTGGCCCAGGCGTCGGCCCGGTGGCGGTGCGGGCGCACCTGGCACCGTTTCTGCCAGGTCACCCCTTCGCCCCCGAGCTGCCCAAGGGCTATCCGGTGTCGTCGGCACCATATGGGTCGGCTTCGATTCTTCCGATCACCTGGGCATACATCCGGATGATGGGGGCTGAGGGACTGCGGGCGGCATCGCTGACAGCGATCACGTCGGCTAACTACATTGCGCGCCGCCTTGACGAGTATTACCCGGTGCTGTACACCGGCGAGAACGGCATGGTCGCCCACGAGTGCATCCTGGACTTGCGCGGTATCACTAAGTTGACCGGTATCACCGTCGACGATGTCGCAAAACGGCTGGCAGACTATGGTTTTCACGCACCAACGATGAGTTTTCCGGTGGCCGGTACGCTCATGGTGGAGCCCACCGAGAGCGAGAGCCTGGCCGAAGTGGACGCCTTCTGCGAGGCCATGATCGGCATCCGCGCCGAGATCGACAAAGTCGGGGCCGGGGAGTGGCCTGTCGACGACAATCCGCTGCGCGGCGCACCGCACACCGCGCAGTGCCTGCTGGCGTCTGATTGGGACCACCCGTATACGCGGGAACAGGCCGCCTACCCGCTCGGCACCGCATTCCGACCCAAGGTTTGGCCCGCGGTACGTCGCATCGACGGCGCCTACGGGGATCGCAACCTGGTCTGCTCATGCCCGCCGGTAGAGGCTTTTGCCTAA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (By similarity). {ECO:0000250}.

Family

GcvP family

GO

glycine decarboxylation via glycine cleavage system GO:0019464 biological_process
glycine cleavage complex GO:0005960 cellular_component
cell wall GO:0005618 cellular_component
cytosol GO:0005829 cellular_component
plasma membrane GO:0005886 cellular_component
pyridoxal phosphate binding GO:0030170 molecular_function
glycine dehydrogenase (decarboxylating) activity GO:0004375 molecular_function
glycine binding GO:0016594 molecular_function

InterPro

UniProt: P9WN53
GenBank: Rv1832
EnsemblBacteria: Rv1832
Mycobrowser: Rv1832

Summary

Name: Probable glycine dehydrogenase (decarboxylating) (EC 1.4.4.2) (Glycine cleavage system P-protein) (Glycine decarboxylase) (Glycine dehydrogenase (aminomethyl-transferring))
Family: GcvP family
Protein Sequence: MSDHSTFADRHIGLDSQAVATMLAVIGVDSLDDLAVKAVPAGILDTLTDTGAAPGLDSLPPAASEAEALAELRALADANTVAVSMIGQGYYDTHTPPVLLRNIIENPAWYTAYTPYQPEISQGRLEALLNFQTLVTDLTGLEIANASMLDEGTAAAEAMTLMHRAARGPVKRVVVDADVFTQTAAVLATRAKPLGIEIVTADLRAGLPDGEFFGVIAQLPGASGRITDWSALVQQAHDRGALVAVGADLLALTLIAPPGEIGADVAFGTTQRFGVPMGFGGPHAGYLAVHAKHARQLPGRLVGVSVDSDGTPAYRLALQTREQHIRRDKATSNICTAQVLLAVLAAMYASYHGAGGLTAIARRVHAHAEAIAGALGDALVHDKYFDTVLARVPGRADEVLARAKANGINLWRVDADHVSVACDEATTDTHVAVVLDAFGVAAAAPAHTDIATRTSEFLTHPAFTQYRTETSMMRYLRALADKDIALDRSMIPLGSCTMKLNAAAEMESITWPEFGRQHPFAPASDTAGLRQLVADLQSWLVLITGYDAVSLQPNAGSQGEYAGLLAIHEYHASRGEPHRDICLIPSSAHGTNAASAALAGMRVVVVDCHDNGDVDLDDLRAKVGEHAERLSALMITYPSTHGVYEHDIAEICAAVHDAGGQVYVDGANLNALVGLARPGKFGGDVSHLNLHKTFCIPHGGGGPGVGPVAVRAHLAPFLPGHPFAPELPKGYPVSSAPYGSASILPITWAYIRMMGAEGLRAASLTAITSANYIARRLDEYYPVLYTGENGMVAHECILDLRGITKLTGITVDDVAKRLADYGFHAPTMSFPVAGTLMVEPTESESLAEVDAFCEAMIGIRAEIDKVGAGEWPVDDNPLRGAPHTAQCLLASDWDHPYTREQAAYPLGTAFRPKVWPAVRRIDGAYGDRNLVCSCPPVEAFA
Mass: 99,511 Da
Length: 941 Aa

Rv1832 doesn't seem to be a targeted by any drug.

Glyoxylate and dicarboxylate metabolism mtu00630
mtu00630
PATHWAY_MAP mtu00630 Glyoxylate and dicarboxylate metabolism

Glycine, serine and threonine metabolism mtu00260
mtu00260
Metabolism; Amino acid metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.