Gene - combatTB X

dop

Type: protein_coding
Name: dop
Locus Name: Rv2112c
Product: Deamidase of pup Dop
Functional Category: Intermediary metabolism and respiration
Location: 2370905..2372569 (- strand)
Gene Length: 1664 bp
Nucleotides: ATGTTCTGGGTCGGCGGGCCTTGCTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTCGGCGGGCGTTGCTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTCGGCGGGCCTAGGCTCTACGGCATGCAGCGGATTATCGGAACGGAGGTCGAGTACGGCATTTCCTCGCCGTCGGACCCGACCGCCAACCCGATCCTCACCTCGACGCAGGCGGTGCTGGCATACGCCGCCGCCGCCGGCATTCAGCGTGCCAAACGCACCCGTTGGGACTACGAGGTGGAATCGCCGCTGCGCGACGCCCGGGGCTTCGATTTGAGTCGCTCGGCCGGGCCGCCGCCGGTGGTCGACGCCGACGAGGTCGGCGCGGCCAACATGATCCTGACCAACGGGGCGCGGCTGTATGTCGACCACGCGCACCCGGAATACTCCGCGCCCGAATGCACCGACCCGCTGGACGCAGTGATCTGGGACAAGGCGGGCGAACGCGTGATGGAGGCCGCTGCCCGCCATGTCGCCAGCGTGCCCGGGGCCGCGAAACTGCAGCTGTACAAGAACAACGTCGACGGCAAGGGAGCCTCCTACGGGTCGCACGAGAACTACCTGATGTCGCGGCAGACACCGTTCTCGGCGATCATCACCGGGCTGACCCCCTTTCTGGTATCCCGGCAGGTGGTGACCGGCTCGGGCCGGGTCGGCATCGGGCCCTCGGGTGATGAGCCCGGCTTCCAGCTATCCCAGCGTTCGGACTACATCGAGGTCGAGGTAGGGCTGGAAACAACGCTCAAGCGCGGCATCATCAACACCCGCGACGAACCGCACGCCGACGCCGACAGGTACCGCCGGCTGCACGTCATCATCGGCGACGCCAACCTTGCCGAGACGTCGACCTATCTGAAGTTGGGTACCACGGCGCTGGTGCTCGACCTGATCGAAGAAGGACCAGCCCACGCAATAGATCTGACCGACCTGGCGCTGGCCCGCCCGGTACATGCGGTGCACGCAATCTCCCGCGATCCGTCGCTGCGAGCGACCGTTGCGCTGGCCGACGGCCGGGAACTGACCGGTCTTGCGCTGCAACGGATCTACCTGGACCGAGTGGCTAAGTTGGTGGATAGCCGCGACCCGGACCCGCGGGCGGCCGACATCGTGGAAACCTGGGCACACGTGCTGGATCAGCTCGAGCGTGACCCGATGGATTGCGCGGAGCTGCTGGACTGGCCGGCCAAACTGCGGCTGCTCGACGGTTTCCGGCAGCGGGAGAACCTGAGCTGGTCGGCGCCCCGGCTGCACCTCGTCGACCTGCAGTACTCCGATGTCCGGCTGGACAAGGGCCTGTACAACCGGCTGGTCGCGCGCGGCTCGATGAAGCGTTTAGTCACCGAACACCAGGTGCTGAGTGCGGTGGAGAACCCGCCGACCGACACCCGCGCGTATTTCCGCGGCGAATGCCTGCGCCGGTTCGGGGCTGATATCGCCGCGGCTAGCTGGGACTCGGTGATCTTCGACCTGGGCGGCGACTCGCTGGTTCGCATCCCGACGCTGGAGCCGTTGCGGGGTAGTAAGGCGCATGTTGGTGCGTTGCTGGATTCGGTGGACAGTGCCGTGGAGCTGGTAGAGCAACTGACCGCTGAGCCTCGCTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target proteins; is thus involved in the recycling of Pup and may function similarly to deubiquitinases (DUBs) in eukaryotes to prevent or promote proteasomal degradation of certain proteins. {ECO:0000269|PubMed:19448618, ECO:0000269|PubMed:20636328, ECO:0000269|PubMed:20705495}.

Family

Pup ligase/Pup deamidase family, Pup deamidase subfamily

GO

metal ion binding GO:0046872 molecular_function
proteasomal protein catabolic process GO:0010498 biological_process
pathogenesis GO:0009405 biological_process
modification-dependent protein catabolic process GO:0019941 biological_process
ATP binding GO:0005524 molecular_function
protein pupylation GO:0070490 biological_process
peptidase activity GO:0008233 molecular_function
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides GO:0016811 molecular_function

InterPro

UniProt: P9WNU9
GenBank: Rv2112c
EnsemblBacteria: Rv2112c
Mycobrowser: Rv2112c

Summary

Name: Pup deamidase/depupylase (EC 3.4.-.-) (EC 3.5.1.119) (Deamidase of protein Pup)
Family: Pup ligase/Pup deamidase family, Pup deamidase subfamily
Protein Sequence: MQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYIEVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRLLDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR
Mass: 55,179 Da
Length: 505 Aa

Rv2112c doesn't seem to be a targeted by any drug.

Rv2112c doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Molecular analysis of the prokaryotic ubiquitin-like protein (Pup) conjugation pathway in Mycobacterium tuberculosis.: Mol Microbiol. 2010 Sep;77(5):1123-35. doi: 10.1111/j.1365-2958.2010.07276.x.
"Depupylation" of prokaryotic ubiquitin-like protein from mycobacterial proteasome substrates.: Mol Cell. 2010 Sep 10;39(5):821-7. doi: 10.1016/j.molcel.2010.07.019. Epub 2010 Aug 12.
Bacterial ubiquitin-like modifier Pup is deamidated and conjugated to substrates by distinct but homologous enzymes.: Nat Struct Mol Biol. 2009 Jun;16(6):647-51. doi: 10.1038/nsmb.1597. Epub 2009 May 17.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.