glnE
- Type
- protein_coding
- Name
- glnE
- Locus Name
-
Rv2221c
- Product
-
Glutamate-ammonia-ligase adenylyltransferase GlnE (glutamine-synthetase adenylyltransferase)
- Functional Category
-
Intermediary metabolism and respiration
- Location
- 2489369..2492353 (- strand)
- Gene Length
- 2984 bp
- Nucleotides
-
GTGGTCGTGACCAAACTCGCCACGCAGCGGCCGAAGTTGCCCAGCGTTGGCCGGCTCGGATTAGTTGACCCCCCTGCTGGTGAGCGTCTGGCTCAGCTGGGGTGGGATCGGCACGAGGATCAGGCGCACGTCGACCTGCTGTGGTCGCTGTCACGCGCTCCGGACGCCGATGCCGCGCTGCGCGCCTTGATCCGGCTGTCGGAGAATCCAGACACCGGATGGGACGAGCTCAACGCGGCTCTGCTGCGCGAACGCAGTCTGCGCGGGCGGCTGTTCTCGGTGCTGGGCTCGTCGCTGGCGTTGGGCGATCACCTGGTCGCCCATCCGCAGTCCTGGAAATTGCTGCGGGGCAAGGTCACACTGCCGTCCCATGACCAGCTGCAGCGGTCGTTCGTCGAGTGCGTCGAGGAATCGGAGGGTATGCCGGGCTCGCTCGTGCACCGATTGCGAACCCAGTACCGCGACTACGTGCTAATGCTGGCCGCTCTCGACCTGGCCGCGACGGTCGAGGACGAACCGGTGCTGCCATTCACCGTGGTGGCCGCACGCCTGGCGGACGCCGCGGACGCCGCTCTGGCGGCGGCGCTGCGCGTGGCCGAGGCGAGCGTGTGCGGCGAGCACCCGCCACCGCGCCTGGCGGTCATCGCGATGGGCAAGTGCGGTGCGCGCGAACTGAACTACGTCAGCGACGTCGATGTCATATTCGTTGCCGAGCGCTCCGACCCGCGCAACGCGCGCGTGGCCAGCGAGATGATGCGGGTGGCCTCGGCGGCCTTTTTCGAGGTGGACGCCGCCCTGCGTCCGGAGGGGCGCAACGGGGAGCTGGTCCGTACGCTCGAGTCGCACATCGCCTACTACCAGCGCTGGGCCAAGACCTGGGAGTTTCAGGCGTTGCTGAAAGCACGGCCAGTCGTTGGCGACGCGGAACTTGGCGAGCGTTACCTGACCGCCTTGATGCCGATGGTGTGGCGAGCCTGCGAGCGCGAAGACTTTGTGGTCGAGGTGCAGGCCATGCGGCGGCGGGTGGAGCAGCTGGTGCCCGCCGATGTCCGCGGCCGCGAGCTCAAACTCGGCAGCGGCGGATTGCGCGACGTGGAGTTCGCCGTACAGCTACTGCAGCTGGTTCATGCCCGTAGCGACGAGTCGTTACGGGTGGCGTCCACGGTGGACGCATTGGCGGCGTTGGGCGAAGGCGGCTACATCGGGCGTGAGGACGCGGCGAACATGACCGCGTCGTATGAGTTCCTCAGGCTGCTCGAGCACCGACTGCAGTTGCAGCGGCTCAAGCGCACCCACCTGCTTCCCGATCCCGAAGACGAGGAGGCAGTGCGCTGGCTGGCGCGCGCGGCCCACATCCGGCCCGATGGCCGAAACGATGCGGCCGGGGTGCTGCGGGAGGAACTCAAGAAGCAGAACGTGCGGGTGTCGAAGTTACACACCAAACTCTTCTATCAACCGCTGCTGGAATCGATCGGCCCGACCGGGTTGGAGATCGCCCACGGCATGACGTTGGAGGCCGCGGGGCGCCGGCTGGCCGCGCTGGGCTACGAGGGACCGCAGACCGCGTTGAAACACATGTCGGCGTTGGTCAATCAAAGCGGCCGGCGCGGACGGGTGCAGTCGGTGCTGCTGCCCAGGCTGCTGGACTGGATGTCGTATGCCCCCGATCCCGACGGCGGACTGCTGGCCTACCGGCGGCTCAGTGAGGCGCTGGCCACCGAAAGCTGGTACCTGGCCACGCTGCGGGACAAGCCCGCGGTGGCCAAGCGGCTCATGCATGTCTTGGGTACCTCGGCGTATGTGCCGGATCTGTTGATGCGCGCGCCGCGGGTCATCCAGCAGTACGAGGACGGGCCTGCGGGCCCGAAGCTGCTCGAGACCGAGCCCGCCGCCGTGGCTCGGGCGCTGATCGCCTCGGCGAGCCGCTACCCCGACCCGGAGCGGGCCATCGCCGGCGCGCGCACGCTGCGTCGTCGAGAGCTGGCCCGCATCGGTTCGGCGGACCTGCTCGGCCTGCTCGAGGTCACCGAGGTGTGCCGGGCGTTGACGTCGGTGTGGGTGGCGGTGCTGCAGGCCGCGCTGGACGTCATGATCCGGGCCAGCCTTCCCGACGACGATCGCGCCCCGGCGGCCATCGCGGTCATCGGCATGGGTCGGCTGGGTGGTGCCGAGTTGGGCTACGGGTCGGATGCCGACGTGATGTTCGTCTGTGAGCCGGCCACCGGCGTCGACGATGCACGGGCGGTGAAATGGTCGACATCGATCGCCGAGCGGGTTCGGGCGCTGCTGGGGACACCCAGCGTCGATCCGCCGCTGGAGCTCGACGCCAATTTGCGACCCGAGGGCCGCAACGGTCCGCTGGTCCGCACCCTGGGGTCCTACGCCGCATACTACGAGCAGTGGGCACAGCCATGGGAGATCCAGGCCCTGCTACGCGCACACGCGGTTGCCGGCGATGCCGAGTTGGGTCAGCGATTCCTACGGATGGTCGACAAAACGCGGTATCCGCCCGACGGTGTGTCCGCTGACTCGGTGCGCGAGATTCGCCGCATCAAGGCCCGTATCGAGTCCGAGCGGTTGCCGCGCGGTGCCGACCCCAACACACACACCAAACTGGGCCGCGGCGGACTGGCCGACATCGAATGGACCGTGCAGTTGCTGCAGCTACAGCATGCGCACCAGGTTCCCGCCCTGCACAACACCTCGACGCTGCAATCCCTGGATGTCATCGCGGCCGCCGATCTGGTTCCCGCAGCCGACGTGGAGCTGCTCCGTCAGGCCTGGCTGACCGCCACCCGGGCCCGCAACGCGCTGGTGTTGGTGCGCGGCAAGCCCACCGACCAGCTGCCGGGACCCGGGCGCCAGCTCAATGCGGTCGCGGTCGCGGCCGGCTGGCGAAACGACGACGGTGGGGAATTCCTGGACAACTACCTACGGGTGACGCGGCGGGCAAAGGCGGTAGTGCGCAAAGTGTTCGGGAGTTG
- Drug Resistance
- Mutations
- Function
- Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell. {ECO:0000255|HAMAP-Rule:MF_00802}.
- Family
-
GlnE family
- GO
-
- regulation of glutamine family amino acid metabolic process GO:0000820 biological_process
- magnesium ion binding GO:0000287 molecular_function
- cell wall GO:0005618 cellular_component
- ATP binding GO:0005524 molecular_function
- cytosol GO:0005829 cellular_component
- plasma membrane GO:0005886 cellular_component
- response to ammonium ion GO:0060359 biological_process
- [glutamine synthetase]-adenylyl-L-tyrosine phosphorylase GO:0047388 molecular_function
- metabolic process GO:0008152 biological_process
- [glutamate-ammonia-ligase] adenylyltransferase activity GO:0008882 molecular_function
- InterPro
Summary
- Name
- Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (ATP:glutamine synthetase adenylyltransferase) (ATase) [Includes: Glutamine synthetase adenylyl-L-tyrosine phosphorylase (EC 2.7.7.89) (Adenylyl removase) (AR) (AT-N); Glutamine synthetase adenylyl transferase (EC 2.7.7.42) (Adenylyl transferase) (AT) (AT-C)]
- Family
- GlnE family
- Protein Sequence
-
MVVTKLATQRPKLPSVGRLGLVDPPAGERLAQLGWDRHEDQAHVDLLWSLSRAPDADAALRALIRLSENPDTGWDELNAALLRERSLRGRLFSVLGSSLALGDHLVAHPQSWKLLRGKVTLPSHDQLQRSFVECVEESEGMPGSLVHRLRTQYRDYVLMLAALDLAATVEDEPVLPFTVVAARLADAADAALAAALRVAEASVCGEHPPPRLAVIAMGKCGARELNYVSDVDVIFVAERSDPRNARVASEMMRVASAAFFEVDAALRPEGRNGELVRTLESHIAYYQRWAKTWEFQALLKARPVVGDAELGERYLTALMPMVWRACEREDFVVEVQAMRRRVEQLVPADVRGRELKLGSGGLRDVEFAVQLLQLVHARSDESLRVASTVDALAALGEGGYIGREDAANMTASYEFLRLLEHRLQLQRLKRTHLLPDPEDEEAVRWLARAAHIRPDGRNDAAGVLREELKKQNVRVSKLHTKLFYQPLLESIGPTGLEIAHGMTLEAAGRRLAALGYEGPQTALKHMSALVNQSGRRGRVQSVLLPRLLDWMSYAPDPDGGLLAYRRLSEALATESWYLATLRDKPAVAKRLMHVLGTSAYVPDLLMRAPRVIQQYEDGPAGPKLLETEPAAVARALIASASRYPDPERAIAGARTLRRRELARIGSADLLGLLEVTEVCRALTSVWVAVLQAALDVMIRASLPDDDRAPAAIAVIGMGRLGGAELGYGSDADVMFVCEPATGVDDARAVKWSTSIAERVRALLGTPSVDPPLELDANLRPEGRNGPLVRTLGSYAAYYEQWAQPWEIQALLRAHAVAGDAELGQRFLRMVDKTRYPPDGVSADSVREIRRIKARIESERLPRGADPNTHTKLGRGGLADIEWTVQLLQLQHAHQVPALHNTSTLQSLDVIAAADLVPAADVELLRQAWLTATRARNALVLVRGKPTDQLPGPGRQLNAVAVAAGWRNDDGGEFLDNYLRVTRRAKAVVRKVFGS
- Mass
- 109,138 Da
- Length
- 994 Aa
Rv2221c doesn't seem to be a targeted by any drug.
Rv2221c doesn't seem to be involved in any pathway.
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.
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- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.