Gene - combatTB X

aceE

Type: protein_coding
Name: aceE
Locus Name: Rv2241
Product: Pyruvate dehydrogenase E1 component AceE (pyruvate decarboxylase) (pyruvate dehydrogenase) (pyruvic dehydrogenase)
Functional Category: Intermediary metabolism and respiration
Location: 2512539..2515244 (+ strand)
Gene Length: 2705 bp
Nucleotides: TGGCGTCGTATTTGCCCGACATTGATCCCGAGGAGACCTCGGAGTGGCTGGAGTCCTTTGACACGCTGCTGCAACGCTGCGGCCCGTCGCGGGCCCGCTACCTGATGTTGCGGCTGCTAGAGCGGGCCGGCGAGCAGCGGGTGGCCATCCCGGCATTGACGTCTACCGACTATGTCAACACCATCCCGACCGAGCTGGAGCCGTGGTTCCCCGGCGACGAAGACGTCGAACGTCGTTATCGAGCGTGGATCAGATGGAATGCGGCCATCATGGTGCACCGTGCGCAACGACCGGGTGTGGGCGTGGGTGGCCATATCTCGACCTACGCGTCGTCCGCGGCGCTCTATGAGGTCGGTTTCAACCACTTCTTCCGCGGCAAGTCGCACCCGGGCGGCGGCGATCAGGTGTTCATCCAGGGCCACGCTTCCCCGGGAATCTACGCGCGCGCCTTCCTCGAAGGGCGGTTGACCGCCGAGCAACTCGACGGATTCCGCCAGGAACACAGCCATGTCGGCGGCGGGTTGCCGTCCTATCCGCACCCGCGGCTCATGCCCGACTTCTGGGAATTCCCCACCGTGTCGATGGGTTTGGGCCCGCTCAACGCCATCTACCAGGCACGGTTCAACCACTATCTGCATGACCGCGGTATCAAAGACACCTCCGATCAACACGTGTGGTGTTTTTTGGGCGACGGCGAGATGGACGAACCCGAGAGCCGTGGGCTGGCCCACGTCGGCGCGCTGGAAGGCTTGGACAACTTGACCTTCGTGATCAACTGCAATCTGCAGCGACTCGACGGCCCGGTGCGCGGCAACGGCAAGATCATCCAGGAGCTGGAGTCGTTCTTCCGCGGTGCCGGCTGGAACGTCATCAAGGTGGTGTGGGGCCGCGAATGGGATGCCCTGCTGCACGCCGACCGCGACGGTGCGCTGGTGAATTTAATGAATACAACACCCGATGGCGATTACCAGACCTATAAGGCCAACGACGGCGGCTACGTGCGTGACCACTTCTTCGGCCGCGACCCACGCACCAAGGCGCTGGTGGAGAACATGAGCGACCAGGATATCTGGAACCTCAAACGGGGCGGCCACGATTACCGCAAGGTTTACGCCGCCTACCGCGCCGCCGTCGACCACAAGGGACAGCCGACGGTGATCCTGGCCAAGACCATCAAAGGCTACGCGCTGGGCAAGCATTTCGAAGGACGCAATGCCACCCACCAGATGAAAAAACTGACCCTGGAAGACCTTAAGGAGTTTCGTGACACGCAGCGGATTCCGGTCAGCGACGCCCAGCTTGAAGAGAATCCGTACCTGCCGCCCTACTACCACCCCGGCCTCAACGCCCCGGAGATTCGTTACATGCTCGACCGGCGCCGGGCCCTCGGGGGCTTTGTTCCCGAGCGCAGGACCAAGTCCAAAGCGCTGACCCTGCCGGGTCGCGACATCTACGCGCCGCTGAAAAAGGGCTCTGGGCACCAGGAGGTGGCCACCACCATGGCGACGGTGCGCACGTTCAAAGAAGTGTTGCGCGACAAGCAGATCGGGCCGCGGATAGTCCCGATCATTCCCGACGAGGCCCGCACCTTCGGGATGGACTCCTGGTTCCCGTCGCTAAAGATCTATAACCGCAATGGCCAGCTGTATACCGCGGTTGACGCCGACCTGATGCTGGCCTACAAGGAGAGCGAAGTCGGGCAGATCCTGCACGAGGGCATCAACGAAGCCGGGTCGGTGGGCTCGTTCATCGCGGCCGGCACCTCGTATGCGACGCACAACGAACCGATGATCCCCATTTACATCTTCTACTCGATGTTCGGCTTCCAGCGCACCGGCGATAGCTTCTGGGCCGCGGCCGACCAGATGGCTCGAGGGTTCGTGCTCGGGGCCACCGCCGGGCGCACCACCCTGACCGGTGAGGGCCTGCAACACGCCGACGGTCACTCGTTGCTGCTGGCCGCCACCAACCCGGCGGTGGTTGCCTACGACCCGGCCTTCGCCTACGAAATCGCCTACATCGTGGAAAGCGGACTGGCCAGGATGTGCGGGGAGAACCCGGAGAACATCTTCTTCTACATCACCGTCTACAACGAGCCGTACGTGCAGCCGCCGGAGCCGGAGAACTTCGATCCCGAGGGCGTGCTGCGGGGTATCTACCGCTATCACGCGGCCACCGAGCAACGCACCAACAAGGCGCAGATCCTGGCCTCCGGGGTAGCGATGCCCGCGGCGCTGCGGGCAGCACAGATGCTGGCCGCCGAGTGGGATGTCGCCGCCGACGTGTGGTCGGTGACCAGTTGGGGCGAGCTAAACCGCGACGGGGTGGCCATCGAGACCGAGAAGCTCCGCCACCCCGATCGGCCGGCGGGCGTGCCCTACGTGACGAGAGCGCTGGAGAATGCTCGGGGCCCGGTGATCGCGGTGTCGGACTGGATGCGCGCGGTCCCCGAGCAGATCCGACCGTGGGTGCCGGGCACATACCTCACGTTGGGCACCGACGGGTTCGGCTTTTCCGACACTCGGCCCGCCGCTCGCCGCTACTTCAACACCGACGCCGAATCCCAGGTGGTCGCGGTTTTGGAGGCGTTGGCGGGCGACGGCGAGATCGACCCATCGGTGCCGGTCGCGGCCGCCCGCCAGTACCGGATCGACGACGTGGCGGCTGCGCCCGAGCAGACCACGGATCCCGGTCCCGGGGCCTAA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). AceE has reductase activity with pyruvate but does not react with 2-oxoglutarate. {ECO:0000269|PubMed:16045627}.

Family

Unknown

GO

magnesium ion binding GO:0000287 molecular_function
glycolytic process GO:0006096 biological_process
cell wall GO:0005618 cellular_component
extracellular region GO:0005576 cellular_component
pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 molecular_function
pyruvate dehydrogenase complex GO:0045254 cellular_component
oxidation-reduction process GO:0055114 biological_process
plasma membrane GO:0005886 cellular_component

InterPro

UniProt: P9WIS9
GenBank: Rv2241
EnsemblBacteria: Rv2241
Mycobrowser: Rv2241

Summary

Name: Pyruvate dehydrogenase E1 component (PDH E1 component) (EC 1.2.4.1)
Family: Unknown
Protein Sequence: MTTDFARHDLAQNSNSASEPDRVRVIREGVASYLPDIDPEETSEWLESFDTLLQRCGPSRARYLMLRLLERAGEQRVAIPALTSTDYVNTIPTELEPWFPGDEDVERRYRAWIRWNAAIMVHRAQRPGVGVGGHISTYASSAALYEVGFNHFFRGKSHPGGGDQVFIQGHASPGIYARAFLEGRLTAEQLDGFRQEHSHVGGGLPSYPHPRLMPDFWEFPTVSMGLGPLNAIYQARFNHYLHDRGIKDTSDQHVWCFLGDGEMDEPESRGLAHVGALEGLDNLTFVINCNLQRLDGPVRGNGKIIQELESFFRGAGWNVIKVVWGREWDALLHADRDGALVNLMNTTPDGDYQTYKANDGGYVRDHFFGRDPRTKALVENMSDQDIWNLKRGGHDYRKVYAAYRAAVDHKGQPTVILAKTIKGYALGKHFEGRNATHQMKKLTLEDLKEFRDTQRIPVSDAQLEENPYLPPYYHPGLNAPEIRYMLDRRRALGGFVPERRTKSKALTLPGRDIYAPLKKGSGHQEVATTMATVRTFKEVLRDKQIGPRIVPIIPDEARTFGMDSWFPSLKIYNRNGQLYTAVDADLMLAYKESEVGQILHEGINEAGSVGSFIAAGTSYATHNEPMIPIYIFYSMFGFQRTGDSFWAAADQMARGFVLGATAGRTTLTGEGLQHADGHSLLLAATNPAVVAYDPAFAYEIAYIVESGLARMCGENPENIFFYITVYNEPYVQPPEPENFDPEGVLRGIYRYHAATEQRTNKAQILASGVAMPAALRAAQMLAAEWDVAADVWSVTSWGELNRDGVAIETEKLRHPDRPAGVPYVTRALENARGPVIAVSDWMRAVPEQIRPWVPGTYLTLGTDGFGFSDTRPAARRYFNTDAESQVVAVLEALAGDGEIDPSVPVAAARQYRIDDVAAAPEQTTDPGPGA
Mass: 103,440 Da
Length: 930 Aa

Rv2241 doesn't seem to be a targeted by any drug.

Pyruvate metabolism mtu00620
mtu00620
PATHWAY_MAP mtu00620 Pyruvate metabolism

Citrate cycle (TCA cycle) mtu00020
mtu00020
Metabolism; Carbohydrate metabolism

Glycolysis / Gluconeogenesis mtu00010
mtu00010
Metabolism; Carbohydrate metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis [corrected] .: PLoS One. 2010 Jan 6;5(1):e8589. doi: 10.1371/journal.pone.0008589.
Mycobacterium tuberculosis appears to lack alpha-ketoglutarate dehydrogenase and encodes pyruvate dehydrogenase in widely separated genes.: Mol Microbiol. 2005 Aug;57(3):859-68. doi: 10.1111/j.1365-2958.2005.04741.x.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.