Gene - combatTB X

mbtB

Type: protein_coding
Name: mbtB
Locus Name: Rv2383c
Product: Phenyloxazoline synthase MbtB (phenyloxazoline synthetase)
Functional Category: Lipid metabolism
Location: 2671593..2675837 (- strand)
Gene Length: 4244 bp
Nucleotides: GTGGTGCATGCTACGGCGTGCTCGGAGATCATTCGCGCCGAGGTCGCAGAGTTGCTCGGTGTCCGCGCCGATGCTCTGCACCCCGGCGCCAACCTCGTTGGTCAGGGCCTGGACTCGATCCGGATGATGTCGCTGGTGGGGCGCTGGCGCCGGAAGGGCATCGCCGTCGACTTCGCCACACTGGCCGCCACACCCACAATCGAGGCCTGGTCCCAACTGGTGTCCGCCGGCACCGGCGTCGCGCCCACGGCAGTTGCCGCACCAGGGGATGCCGGGTTATCCCAGGAAGGCGAGCCTTTCCCGCTGGCGCCGATGCAGCACGCGATGTGGGTCGGCCGCCACGACCACCAACAGCTCGGCGGGGTAGCTGGGCACCTCTACGTCGAGTTCGATGGTGCCCGGGTCGATCCGGACAGGCTCCGCGCGGCGGCCACCCGGTTGGCGCTGCGGCACCCGATGCTGCGGGTGCAGTTCTTGCCCGACGGCACCCAGCGCATCCCGCCGGCGGCGGGATCTCGCGACTTTCCCATCAGTGTTGCCGACCTGCGTCACGTGGCCCCGGATGTCGTCGATCAGCGGTTGGCGGGGATCCGCGACGCCAAATCGCACCAGCAGCTCGACGGTGCGGTATTCGAACTTGCGTTGACGTTATTACCGGGGGAGCGCACCCGCCTACATGTCGACCTGGACATGCAGGCCGCTGACGCGATGAGCTACCGCATCTTGCTGGCCGACCTGGCGGCCCTCTATGACGGCCGTGAACCGCCGGCACTGGGCTACACCTACCGGGAATACCGGCAGGCTATCGAGGCGGAGGAGACGCTGCCCCAACCGGTTCGCGACGCCGACCGGGACTGGTGGGCGCAGCGCATCCCGCAGCTGCCGGATCCGCCCGCGCTGCCTACCCGGGCCGGCGGCGAACGCGACCGGCGCCGCAGCACCCGGCGCTGGCACTGGCTAGACCCGCAGACCCGCGACGCGTTGTTCGCCCGAGCCCGGGCCCGCGGCATCACCCCGGCGATGACGCTGGCCGCGGCCTTTGCCAACGTGCTGGCGCGTTGGTCAGCGTCGTCGCGGTTCCTGCTGAACCTGCCGTTGTTCAGTCGCCAGGCCCTGCATCCAGACGTCGACCTGCTGGTCGGTGACTTCACCTCCTCGCTGTTGCTCGACGTCGATCTGACCGGTGCGCGCACGGCGGCGGCGCGGGCGCAGGCGGTGCAGGAAGCCCTGCGCAGCGCCGCAGGCCACAGTGCATACCCCGGGCTGTCTGTGCTGCGTGACCTCAGCCGCCACCGTGGCACCCAGGTGCTGGCACCGGTGGTATTCACCAGCGCGCTGGGGCTCGGCGACCTTTTCTGCCCGGACGTCACCGAGCAATTCGGCACACCCGGATGGATCATTTCGCAGGGGCCCCAGGTGCTGCTCGACGCCCAGGTCACCGAGTTCGACGGCGGTGTGCTGGTGAACTGGGATGTCCGCGAGGGGGTCTTTGCACCCGGCGTCATCGACGCCATGTTCACCCACCAGGTCGACGAATTGCTCCGGTTGGCCGCCGGGGACGACGCCTGGGATGCGCCGAGCCCGTCCGCGCTACCCGCCGCGCAACGCGCGGTGCGCGCGGCGCTGAACGGTCGCACCGCCGCCCCCAGCACCGAGGCGCTGCACGACGGGTTTTTCCGCCAGGCCCAACAGCAGCCCGACGCGCCGGCGGTGTTCGCCAGTTCCGGCGACTTGAGCTACGCCCAACTGCGCGACCAGGCATCGGCGGTGGCCGCGGCGCTGCGTGCTGCGGGCCTACGAGTCGGCGACACCGTCGCGGTGCTGGGTCCGAAAACGGGCGAACAAGTGGCGGCTGTGCTGGGGATTTTGGCCGCCGGCGGGGTCTATCTGCCGATCGGCGTCGACCAGCCCCGCGACCGCGCGGAGCGCATCCTGGCGACCGGTTCGGTCAACTTAGCGCTCGTTTGCGGCCCGCCATGCCAAGTGCGGGTGCCGGTCCCGACGCTGTTGCTGGCTGACGTGCTTGCCGCCGCGCCGGCAGAATTCGTGCCCGGGCCTAGCGATCCCACCGCGCTCGCCTATGTGTTGTTCACCTCGGGCTCAACCGGGGAACCCAAGGGTGTCGAGGTGGCGCACGACGCCGCGATGAACACCGTGGAGACCTTCATCCGGCACTTCGAGCTAGGCGCCGCAGATCGCTGGCTTGCCCTGGCGACGCTGGAGTGCGACATGTCGGTGCTGGACATCTTCGCCGCCCTGCGCTCCGGCGGAGCGATCGTGGTGGTCGACGAAGCGCAGCGCCGCGATCCCGACGCCTGGGCCCGGCTTATCGACACTTACGAGGTCACGGCGTTGAATTTCATGCCGGGCTGGTTGGACATGCTGCTCGAAGTCGGCGGGGGCCGGCTGTCGTCGCTGCGAGCGGTGGCCGTCGGCGGCGACTGGGTGCGTCCCGACCTGGCCCGCCGCCTGCAGGTGCAAGCGCCGAGCGCACGGTTCGCGGGGTTGGGTGGAGCCACCGAAACCGCGGTCCACGCAACCATTTTCGAGGTCCAGGACGCGGCCAATCTGCCACCGGACTGGGCCTCGGTGCCATACGGCGTCCCGTTTCCCAACAACGCCTGCCGGGTAGTGGCCGACAGCGGCGACGACTGCCCCGATTGGGTGGCCGGTGAGTTGTGGGTGTCCGGTCGCGGAATCGCCCGGGGTTACCGTGGCCGTCCCGAGCTGACCGCGGAGCGCTTCGTCGAGCATGACGGCCGCACCTGGTATCGCACCGGTGATCTGGCCCGCTACTGGCACGACGGCACCCTGGAGTTCGTCGGCCGTGCCGATCACCGCGTCAAGATCAGCGGGTACCGCGTCGAACTCGGCGAGATCGAAGCCGCGCTGCAGCGCTTGCCCGGTGTGCACGCGGCGGCGGCCACCGTGCTTCCTGGCGGGTCCGATGTGCTGGCCGCGGCGGTCTGCGTCGACGATGCCGGCGTGACCGCGGAGTCGATTCGACAGCAGCTCGCCGATCTGGTGCCCGCGCACATGATTCCGCGCCACGTCACGCTGCTAGACCGCATCCCCTTTACCGACAGCGGCAAGATCGACCGCGCGGAGGTTGGCGCCCTGCTTGCCGCCGAGGTCGAGCGGTCTGGCGACAGATCGGCACCCTATGCGGCGCCGCGAACGGTGCTTCAGCGGGCACTGCGCCGCATCGTCGCGGACATCCTGGGCCGTGCCAACGATGCCGTGGGCGTGCACGACGACTTCTTTGCCCTGGGCGGCGATTCCGTGCTTGCGACCCAGGTCGTCGCCGGTATCCGGCGGTGGCTGGATTCGCCGAGCCTGATGGTCGCCGACATGTTTGCCGCCAGGACCATTGCCGCGTTGGCCCAGTTGCTCACCGGTCGGGAAGCCAACGCCGACCGACTCGAACTGGTCGCCGAGGTGTACTTAGAAATCGCGAATATGACAAGTGCCGACGTGATGGCGGCGCTCGATCCGATCGAGCAGCCCGCACAGCCAGCCTTCAAACCGTGGGTGAAGCGGTTCACCGGTACTGACAAGCCCGGCGCGGTGCTGGTGTTTCCACACGCCGGCGGCGCTGCCGCGGCTTACCGGTGGTTGGCAAAATCGTTAGTGGCCAACGACGTTGACACGTTCGTGGTGCAGTACCCGCAGCGGGCTGACCGGCGCAGCCACCCGGCGGCAGACAGCATCGAGGCGCTGGCGCTCGAGCTGTTCGAGGCGGGCGACTGGCACCTGACGGCTCCGCTGACGCTGTTCGGCCATTGCATGGGTGCGATAGTGGCCTTCGAGTTCGCTCGCCTCGCCGAGCGCAATGGCGTGCCGGTACGTGCACTGTGGGCTTCCTCCGGTCAGGCTCCGTCGACGGTGGCCGCGTCCGGACCGTTGCCGACCGCCGACCGTGACGTCCTGGCCGACATGGTGGATCTTGGCGGCACCGATCCCGTGCTGCTCGAGGACGAGGAATTCGTTGAACTGCTGGTGCCGGCGGTCAAGGCCGACTATCGGGCCCTCAGCGGCTATTCATGTCCACCCGACGTGCGCATCCGCGCCAACATCCACGCGGTCGGCGGCAACCGCGACCACCGCATCAGCCGGGAGATGTTGACCAGTTGGGAGACTCACACCTCCGGTCGCTTCACGCTGTCGCACTTTGATGGTGGTCACTTCTATCTCAACGATCACCTCGACGCCGTGGCCCGGATGGTGAGTGCCGATGTCCGATA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the initial steps of the mycobactin biosynthetic pathway. Putatively couples activated salicylic acid with serine or threonine and cyclizes this precursor to the hydroxyphenyloxazoline ring system present in this class of siderophores. Essential for growth in macrophages. {ECO:0000269|PubMed:10655517}.

Family

ATP-dependent AMP-binding enzyme family, MbtB subfamily

GO

siderophore biosynthetic process from catechol GO:0019540 biological_process
cellular response to iron ion starvation GO:0010106 biological_process
pathogenesis GO:0009405 biological_process
cell wall GO:0005618 cellular_component
response to host immune response GO:0052572 biological_process
phosphopantetheine binding GO:0031177 molecular_function
plasma membrane GO:0005886 cellular_component
cytoplasm GO:0005737 cellular_component
ligase activity GO:0016874 molecular_function
hydrolase activity, acting on ester bonds GO:0016788 molecular_function
acyl carrier activity GO:0000036 molecular_function

InterPro

UniProt: P9WQ63
GenBank: Rv2383c
EnsemblBacteria: Rv2383c
Mycobrowser: Rv2383c

Summary

Name: Phenyloxazoline synthase MbtB (EC 6.3.2.-) (Mycobactin synthetase protein B)
Family: ATP-dependent AMP-binding enzyme family, MbtB subfamily
Protein Sequence: MVHATACSEIIRAEVAELLGVRADALHPGANLVGQGLDSIRMMSLVGRWRRKGIAVDFATLAATPTIEAWSQLVSAGTGVAPTAVAAPGDAGLSQEGEPFPLAPMQHAMWVGRHDHQQLGGVAGHLYVEFDGARVDPDRLRAAATRLALRHPMLRVQFLPDGTQRIPPAAGSRDFPISVADLRHVAPDVVDQRLAGIRDAKSHQQLDGAVFELALTLLPGERTRLHVDLDMQAADAMSYRILLADLAALYDGREPPALGYTYREYRQAIEAEETLPQPVRDADRDWWAQRIPQLPDPPALPTRAGGERDRRRSTRRWHWLDPQTRDALFARARARGITPAMTLAAAFANVLARWSASSRFLLNLPLFSRQALHPDVDLLVGDFTSSLLLDVDLTGARTAAARAQAVQEALRSAAGHSAYPGLSVLRDLSRHRGTQVLAPVVFTSALGLGDLFCPDVTEQFGTPGWIISQGPQVLLDAQVTEFDGGVLVNWDVREGVFAPGVIDAMFTHQVDELLRLAAGDDAWDAPSPSALPAAQRAVRAALNGRTAAPSTEALHDGFFRQAQQQPDAPAVFASSGDLSYAQLRDQASAVAAALRAAGLRVGDTVAVLGPKTGEQVAAVLGILAAGGVYLPIGVDQPRDRAERILATGSVNLALVCGPPCQVRVPVPTLLLADVLAAAPAEFVPGPSDPTALAYVLFTSGSTGEPKGVEVAHDAAMNTVETFIRHFELGAADRWLALATLECDMSVLDIFAALRSGGAIVVVDEAQRRDPDAWARLIDTYEVTALNFMPGWLDMLLEVGGGRLSSLRAVAVGGDWVRPDLARRLQVQAPSARFAGLGGATETAVHATIFEVQDAANLPPDWASVPYGVPFPNNACRVVADSGDDCPDWVAGELWVSGRGIARGYRGRPELTAERFVEHDGRTWYRTGDLARYWHDGTLEFVGRADHRVKISGYRVELGEIEAALQRLPGVHAAAATVLPGGSDVLAAAVCVDDAGVTAESIRQQLADLVPAHMIPRHVTLLDRIPFTDSGKIDRAEVGALLAAEVERSGDRSAPYAAPRTVLQRALRRIVADILGRANDAVGVHDDFFALGGDSVLATQVVAGIRRWLDSPSLMVADMFAARTIAALAQLLTGREANADRLELVAEVYLEIANMTSADVMAALDPIEQPAQPAFKPWVKRFTGTDKPGAVLVFPHAGGAAAAYRWLAKSLVANDVDTFVVQYPQRADRRSHPAADSIEALALELFEAGDWHLTAPLTLFGHCMGAIVAFEFARLAERNGVPVRALWASSGQAPSTVAASGPLPTADRDVLADMVDLGGTDPVLLEDEEFVELLVPAVKADYRALSGYSCPPDVRIRANIHAVGGNRDHRISREMLTSWETHTSGRFTLSHFDGGHFYLNDHLDAVARMVSADVR
Mass: 151,634 Da
Length: 1414 Aa

Rv2383c doesn't seem to be a targeted by any drug.

Rv2383c doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages.: Mol Microbiol. 2001 Nov;42(3):851-65.
The salicylate-derived mycobactin siderophores of Mycobacterium tuberculosis are essential for growth in macrophages.: Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1252-7. doi: 10.1073/pnas.97.3.1252.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.