Gene - combatTB X

sirA

Type: protein_coding
Name: sirA
Locus Name: Rv2391
Product: Ferredoxin-dependent sulfite reductase SirA
Functional Category: Intermediary metabolism and respiration
Location: 2684679..2686370 (+ strand)
Gene Length: 1691 bp
Nucleotides: TGTCCGCGAAGGAGAACCCCCAAATGACCACTGCACGTCCCGCCAAGGCTCGAAATGAGGGCCAGTGGGCGCTGGGACATCGCGAGCCACTCAACGCCAACGAAGAGCTGAAGAAGGCCGGCAACCCGCTCGACGTGCGGGAGCGCATCGAAAACATCTACGCCAAACAGGGTTTCGACAGCATCGACAAGACCGACCTGCGAGGGCGCTTTCGCTGGTGGGGCCTGTACACCCAGCGTGAGCAGGGCTACGACGGCACCTGGACCGGTGACGACAACATCGACAAGCTCGAGGCCAAATACTTCATGATGCGGGTGCGTTGCGACGGCGGCGCGCTCTCGGCTGCCGCGCTGCGCACGCTGGGCCAGATCTCGACGGAGTTCGCGCGCGATACCGCCGATATCTCCGACCGGCAGAACGTGCAATACCACTGGATCGAAGTGGAAAACGTCCCTGAAATCTGGCGACGGTTAGACGATGTCGGACTGCAGACCACCGAGGCGTGCGGTGACTGCCCGCGGGTAGTGCTGGGCTCGCCGTTGGCCGGCGAGTCGCTCGACGAAGTGCTCGACCCGACCTGGGCGATCGAGGAGATCGTGCGTCGCTACATCGGCAAGCCCGACTTCGCCGACTTGCCGCGCAAGTACAAGACCGCCATCTCTGGCCTGCAGGACGTCGCGCACGAGATCAACGACGTCGCCTTCATCGGCGTCAACCATCCCGAGCACGGACCAGGCCTGGATCTGTGGGTGGGCGGTGGACTGTCGACCAACCCGATGCTGGCCCAGCGGGTCGGCGCCTGGGTTCCACTGGGCGAAGTGCCCGAGGTGTGGGCGGCGGTCACCTCGGTGTTTCGCGACTACGGCTACCGGCGACTGCGCGCCAAGGCCCGGCTGAAATTTCTGATCAAAGACTGGGGCATAGCGAAGTTCCGCGAAGTGCTCGAAACCGAGTACCTCAAGCGTCCGCTGATCGACGGTCCGGCCCCCGAACCGGTCAAGCATCCGATCGACCACGTCGGGGTGCAACGACTCAAGAACGGGCTCAACGCCGTCGGAGTCGCCCCCATCGCCGGGCGGGTATCGGGCACCATCCTCACGGCGGTCGCCGACCTGATGGCGCGGGCCGGTTCCGACCGGATCCGGTTCACCCCCTACCAGAAGCTGGTCATCCTCGACATTCCGGACGCCTTGCTCGACGACTTGATCGCCGGTCTGGACGCGCTGGGGCTGCAGTCGCGCCCGTCGCATTGGCGCCGGAACTTGATGGCGTGCAGCGGGATTGAGTTCTGCAAGTTGTCATTCGCCGAAACCCGGGTTCGAGCACAGCATTTGGTGCCCGAGCTGGAACGCCGGCTTGAGGACATCAACTCGCAGCTCGACGTACCGATCACCGTCAACATCAACGGCTGCCCGAACTCATGTGCGCGAATTCAAATCGCCGACATCGGATTCAAGGGACAGATGATCGACGACGGACACGGCGGCTCCGTCGAAGGCTTCCAGGTGCATCTGGGCGGACACCTCGGCCTGGATGCCGGATTCGGCCGCAAACTGCGCCAGCACAAGGTCACCAGTGACGAACTCGGCGACTACATCGACCGGGTGGTGCGCAACTTCGTCAAACACCGCAGCGAAGGTGAACGCTTCGCGCAGTGGGTCATCCGGGCCGAGGAGGACGACCTGCGATGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the reduction of sulfite to sulfide, a step in the biosynthesis of sulfur-containing amino acids and cofactors. {ECO:0000269|PubMed:15917234}.

Family

Nitrite and sulfite reductase 4Fe-4S domain family

GO

metal ion binding GO:0046872 molecular_function
sulfite reductase activity GO:0016002 molecular_function
cysteine biosynthetic process from serine GO:0006535 biological_process
sulfate assimilation GO:0000103 biological_process
4 iron, 4 sulfur cluster binding GO:0051539 molecular_function
cytosol GO:0005829 cellular_component
cell wall GO:0005618 cellular_component
sulfite reductase (ferredoxin) activity GO:0050311 molecular_function
heme binding GO:0020037 molecular_function

InterPro

UniProt: P9WJ03
GenBank: Rv2391
EnsemblBacteria: Rv2391
Mycobrowser: Rv2391

Summary

Name: Sulfite reductase [ferredoxin] (EC 1.8.7.1)
Family: Nitrite and sulfite reductase 4Fe-4S domain family
Protein Sequence: MTTARPAKARNEGQWALGHREPLNANEELKKAGNPLDVRERIENIYAKQGFDSIDKTDLRGRFRWWGLYTQREQGYDGTWTGDDNIDKLEAKYFMMRVRCDGGALSAAALRTLGQISTEFARDTADISDRQNVQYHWIEVENVPEIWRRLDDVGLQTTEACGDCPRVVLGSPLAGESLDEVLDPTWAIEEIVRRYIGKPDFADLPRKYKTAISGLQDVAHEINDVAFIGVNHPEHGPGLDLWVGGGLSTNPMLAQRVGAWVPLGEVPEVWAAVTSVFRDYGYRRLRAKARLKFLIKDWGIAKFREVLETEYLKRPLIDGPAPEPVKHPIDHVGVQRLKNGLNAVGVAPIAGRVSGTILTAVADLMARAGSDRIRFTPYQKLVILDIPDALLDDLIAGLDALGLQSRPSHWRRNLMACSGIEFCKLSFAETRVRAQHLVPELERRLEDINSQLDVPITVNINGCPNSCARIQIADIGFKGQMIDDGHGGSVEGFQVHLGGHLGLDAGFGRKLRQHKVTSDELGDYIDRVVRNFVKHRSEGERFAQWVIRAEEDDLR
Mass: 62,112 Da
Length: 555 Aa

Rv2391 doesn't seem to be a targeted by any drug.

Cysteine synthesis from O-acetylserine R-MTU-936721
Mycobacterium tuberculosis
R-MTU-936721

Sulfur metabolism mtu00920
mtu00920
Metabolism; Energy metabolism

Siroheme- and [Fe4-S4]-dependent NirA from Mycobacterium tuberculosis is a sulfite reductase with a covalent Cys-Tyr bond in the active site.: J Biol Chem. 2005 Jul 22;280(29):27319-28. doi: 10.1074/jbc.M502560200. Epub 2005 May 24.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.