Gene - combatTB X

lepA

Type: protein_coding
Name: lepA
Locus Name: Rv2404c
Product: Probable GTP-binding protein LepA (GTP-binding elongation factor)
Functional Category: Intermediary metabolism and respiration
Location: 2701287..2703248 (- strand)
Gene Length: 1961 bp
Nucleotides: GTGCGGACGCCATGCTCGCAGCATAGACGCGATCGACCGAGCGCGATTGGGTCGCAGCTGCCCGACGCGGATACCCTGGATACCCGTCAACCCCCGCTCCAGGAGATTCCCATCAGCAGTTTCGCCGACAAAACCTTCACTGCGCCGGCGCAGATTAGGAACTTTTGCATCATCGCCCACATCGACCACGGCAAGTCAACGTTGGCCGACCGGATGCTGCAGCTCACCGGCGTGGTCGACGAACGCTCTATGCGGGCCCAGTACTTGGATCGGATGGACATCGAGCGGGAACGCGGCATCACCATCAAGGCCCAGAATGTGCGGCTACCCTGGCGGGTCGACAAGACCGACTACGTGCTGCATCTGATCGACACCCCGGGCCATGTCGACTTCACCTACGAAGTCTCGCGTGCGCTGGAGGCTTGCGAGGGTGCGGTGCTGTTGGTCGATGCCGCGCAAGGCATCGAGGCGCAGACGCTGGCGAACCTCTACCTGGCGTTGGACCGCGACCTGCACATCATCCCGGTGCTCAACAAGATCGACCTGCCCGCGGCCGACCCGGACCGTTACGCCGCCGAGATGGCACACATCATCGGCTGCGAGCCGGCCGAGGTGTTGCGGGTGTCCGGCAAAACCGGCGAAGGCGTGTCCGACCTGCTCGACGAGGTGGTCCGACAGGTGCCGCCCCCTCAGGGCGACGCCGAGGCACCTACCCGCGCAATGATTTTCGACTCCGTTTACGACATCTACCGCGGAGTGGTGACCTACGTCCGCGTCGTCGACGGCAAGATCAGCCCCCGTGAGCGCATCATGATGATGTCCACCGGCGCCACCCACGAGCTGCTTGAGGTCGGCATCGTCTCACCCGAGCCGAAACCGTGTGAGGGCCTCGGTGTCGGCGAGGTGGGTTACCTGATCACCGGCGTCAAGGACGTCCGCCAGTCCAAGGTGGGCGACACCGTGACCAGCCTCTCCCGGGCCCGCGGGGCTGCCGCCGAAGCGTTGACCGGCTACCGTGAGCCCAAACCGATGGTCTACTCGGGGTTGTATCCGGTCGACGGTTCGGACTACCCGAACCTGCGCGACGCCCTGGACAAGCTGCAGCTCAACGACGCGGCCTTGACCTATGAGCCGGAAACCTCGGTGGCGCTGGGTTTTGGGTTCCGGTGCGGTTTCTTGGGCCTGCTGCACATGGAGATCACCCGTGAGCGCCTTGAGCGCGAGTTCGGCCTGGACCTGATCTCGACCTCACCCAATGTGGTGTATCGGGTGCACAAAGACGACGGCACCGAAATCCGGGTGACCAATCCGTCGGACTGGCCGGAGGGCAAGATCCGCACGGTCTACGAGCCCGTCGTCAAGACCACCATCATCGCGCCCAGCGAGTTCATCGGCACCATCATGGAGCTGTGCCAGTCGCGCCGCGGCGAGTTGGGCGGCATGGACTATCTCTCCCCCGAGCGGGTGGAACTGCGCTACACCATGCCGCTCGGCGAGATCATCTTCGACTTCTTCGACGCACTGAAATCGCGCACCCGCGGCTATGCCAGCCTCGACTACGAGGAGGCCGGCGAACAGGAGGCCGCGCTGGTCAAGGTCGACATCCTGCTGCAGGGCGAGGCGGTGGATGCGTTCAGCGCGATCGTGCACAAGGACACGGCGTATGCCTACGGCAACAAGATGACCACCAAACTCAAGGAGCTGATCCCGCGCCAGCAGTTCGAGGTGCCGGTGCAGGCCGCCATCGGATCGAAAATCATTGCCCGCGAAAACATCCGCGCGATCCGCAAGGACGTGTTGTCCAAGTGCTACGGCGGCGACATCACGCGCAAACGCAAGCTGCTGGAGAAGCAGAAGGAAGGCAAGAAGCGGATGAAGACCATCGGGCGCGTCGAGGTGCCACAGGAGGCTTTCGTCGCGGCATTGTCCACCGATGCCGCCGGGGACAAAGGCAAGAAGTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner. {ECO:0000255|HAMAP-Rule:MF_00071, ECO:0000269|PubMed:22578863}.

Family

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, LepA subfamily

GO

translation elongation factor activity GO:0003746 molecular_function
GTPase activity GO:0003924 molecular_function
ribosome binding GO:0043022 molecular_function
cell wall GO:0005618 cellular_component
GTP binding GO:0005525 molecular_function
cytosol GO:0005829 cellular_component
plasma membrane GO:0005886 cellular_component
positive regulation of translation GO:0045727 biological_process

InterPro

UniProt: P9WK97
GenBank: Rv2404c
EnsemblBacteria: Rv2404c
Mycobrowser: Rv2404c

Summary

Name: Elongation factor 4 (EF-4) (EC 3.6.5.n1) (Ribosomal back-translocase LepA)
Family: TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, LepA subfamily
Protein Sequence: MRTPCSQHRRDRPSAIGSQLPDADTLDTRQPPLQEIPISSFADKTFTAPAQIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSMRAQYLDRMDIERERGITIKAQNVRLPWRVDKTDYVLHLIDTPGHVDFTYEVSRALEACEGAVLLVDAAQGIEAQTLANLYLALDRDLHIIPVLNKIDLPAADPDRYAAEMAHIIGCEPAEVLRVSGKTGEGVSDLLDEVVRQVPPPQGDAEAPTRAMIFDSVYDIYRGVVTYVRVVDGKISPRERIMMMSTGATHELLEVGIVSPEPKPCEGLGVGEVGYLITGVKDVRQSKVGDTVTSLSRARGAAAEALTGYREPKPMVYSGLYPVDGSDYPNLRDALDKLQLNDAALTYEPETSVALGFGFRCGFLGLLHMEITRERLEREFGLDLISTSPNVVYRVHKDDGTEIRVTNPSDWPEGKIRTVYEPVVKTTIIAPSEFIGTIMELCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDALKSRTRGYASLDYEEAGEQEAALVKVDILLQGEAVDAFSAIVHKDTAYAYGNKMTTKLKELIPRQQFEVPVQAAIGSKIIARENIRAIRKDVLSKCYGGDITRKRKLLEKQKEGKKRMKTIGRVEVPQEAFVAALSTDAAGDKGKK
Mass: 72,396 Da
Length: 653 Aa

Rv2404c doesn't seem to be a targeted by any drug.

Rv2404c doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Cloning and characterization of GTP-binding proteins of Mycobacterium tuberculosis H(37)Rv.: Enzyme Microb Technol. 2008 Jan;42(2):138-44. doi: 10.1016/j.enzmictec.2007.08.008. Epub 2007 Sep 1.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.