Function

Effector that is released into the host cell and affects host immune responses; it negatively modulates inflammation, macrophage autophagy, and cell death through redox-dependent signaling (PubMed:17259625, PubMed:21187903). Acts as an acetyltransferase. Acetylates 'Lys-55' of dual-specificity protein phosphatase 16 (DUSP16)/mitogen-activated protein kinase phosphatase-7 (MKP-7), a JNK-specific phosphatase; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis (PubMed:22547814). Inhibits Con A-mediated T-cell proliferation in vitro (PubMed:17449476). Treatment of T-cells with Eis inhibits ERK1/2, JAK pathway, and subsequent production of tumor necrosis factor-alpha (TNF-alpha) and interleukin-4 (IL-4); on the contrary, there is increased production of interferon-gamma (IFN-gamma) and interleukin-10 (IL-10), which indicates that immunity in response to Eis treatment is skewed away from a protective T(H)1 response and Eis disturbs the cross regulation of T-cells (PubMed:17449476). When expressed in M.smegmatis, enhances intracellular survival of the bacteria in host macrophages during infection (PubMed:10629183). {ECO:0000269|PubMed:10629183, ECO:0000269|PubMed:17259625, ECO:0000269|PubMed:17449476, ECO:0000269|PubMed:21187903, ECO:0000269|PubMed:22547814}.; FUNCTION: Can also acetylate multiple amine groups of many aminoglycoside (AG) antibiotics, leading to their inactivation, and thus contributes to drug resistance (PubMed:19906990, PubMed:21628583, PubMed:24106131). Is also able to acetylate and deactivate the cyclic peptide antibiotic capreomycin, but not the other anti-tuberculous drugs isoniazid and pyrazinamide (PubMed:23233486). Acetylates kanamycin (KAN) more efficiently than amikacin (AMK), even though Eis seems to bind AMK with higher affinity (PubMed:19906990). Does not acetylate and inactivate streptomycin, apramycin and spectinomycin (PubMed:19906990, PubMed:21628583). {ECO:0000269|PubMed:19906990, ECO:0000269|PubMed:21628583, ECO:0000269|PubMed:23233486, ECO:0000269|PubMed:24106131}.

Family

Acetyltransferase Eis family

GO

• host extracellular space GO:0043655 cellular_component
• modulation by symbiont of host programmed cell death GO:0052040 biological_process
• positive regulation by symbiont of host inflammatory response GO:0052035 biological_process
• identical protein binding GO:0042802 molecular_function
• modulation by symbiont of host innate immune response GO:0052167 biological_process
• modulation by symbiont of defense-related host reactive oxygen species production GO:0052164 biological_process
• aminoglycoside antibiotic catabolic process GO:0030649 biological_process
• bacterial extracellular vesicle GO:0097691 cellular_component
• aminoglycoside N-acetyltransferase activity GO:0034069 molecular_function
• response to antibiotic GO:0046677 biological_process
• host cell cytoplasmic vesicle GO:0044161 cellular_component
• growth of symbiont in host cell GO:0044119 biological_process
• growth of symbiont in host organelle GO:0044121 biological_process

InterPro

• IPR041380
• IPR016181
• IPR025559
• IPR000182
• IPR022902
• IPR036527

UniProt

P9WFK7

GenBank

Rv2416c

EnsemblBacteria

Rv2416c

Mycobrowser

Rv2416c

Rv2416c doesn't seem to be a targeted by any drug.

Rv2416c doesn't seem to be involved in any pathway.