ldtB
- Type
- protein_coding
- Name
- ldtB
- Locus Name
-
Rv2518c
- Product
-
Probable L,D-transpeptidase LdtB
- Functional Category
-
Cell wall and cell processes
- Location
- 2834109..2835335 (- strand)
- Gene Length
- 1226 bp
- Nucleotides
-
ATGCCAAAGGTGGGGATTGCAGCTCAGGCGGGTAGGACCAGGGTTCGGAGAGCCTGGTTAACCGCGCTGATGATGACCGCCGTGATGATCGGTGCCGTCGCATGCGGCAGCGGCCGCGGACCCGCACCGATCAAGGTCATCGCGGACAAGGGCACGCCGTTCGCCGATCTGCTGGTGCCCAAGCTCACCGCGTCGGTCACCGACGGCGCCGTCGGCGTCACCGTGGATGCACCGGTGTCGGTGACCGCCGCCGACGGTGTGCTGGCGGCCGTCACCATGGTCAACGACAACGGCAGGCCGGTGGCCGGTCGACTCAGCCCCGACGGACTGCGCTGGTCGACCACTGAGCAGCTCGGCTACAACAGGCGCTACACGCTGAACGCGACGGCGCTTGGACTGGGTGGCGCGGCGACCCGCCAGCTGACCTTCCAGACCAGTTCCCCCGCGCACCTGACCATGCCCTACGTCATGCCCGGTGATGGGGAGGTCGTGGGTGTCGGCGAGCCGGTGGCGATCCGATTCGACGAGAACATCGCTGACCGGGGCGCGGCCGAGAAGGCCATCAAGATCACCACCAACCCGCCCGTGGAGGGCGCGTTCTACTGGCTGAATAACCGTGAAGTGCGTTGGCGCCCAGAGCATTTCTGGAAGCCAGGTACGGCCGTTGATGTGGCGGTCAACACCTACGGCGTCGACTTGGGCGAGGGAATGTTCGGCGAGGACAACGTGCAGACGCACTTCACCATCGGCGACGAGGTGATCGCGACCGCCGACGACAACACCAAGATACTGACCGTGCGGGTGAATGGCGAGGTCGTAAAGTCCATGCCGACGTCGATGGGCAAGGACAGCACCCCGACGGCCAACGGCATATACATCGTCGGCTCGCGGTACAAGCACATCATCATGGACTCGTCCACCTACGGCGTACCCGTCAACTCGCCCAACGGATATCGCACCGATGTCGACTGGGCCACCCAGATCTCCTACAGCGGTGTCTTCGTGCACTCAGCGCCGTGGTCGGTGGGGGCTCAGGGCCACACCAACACCAGCCATGGCTGCCTGAACGTCAGCCCGAGCAACGCGCAGTGGTTCTACGACCATGTCAAGCGCGGCGACATCGTCGAGGTGGTCAATACCGTGGGTGGCACCCTGCCGGGGATCGACGGGCTCGGCGATTGGAACATTCCGTGGGACCAGTGGCGCGCCGGTAACGCCAAGGCGTA
- Drug Resistance
- Mutations
- Function
- Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. {ECO:0000269|PubMed:24041897}.
- Family
-
Unknown
- GO
-
- peptidoglycan L,D-transpeptidase activity GO:0071972 molecular_function
- metal ion binding GO:0046872 molecular_function
- cell wall organization GO:0071555 biological_process
- peptidoglycan-protein cross-linking GO:0018104 biological_process
- regulation of cell shape GO:0008360 biological_process
- plasma membrane GO:0005886 cellular_component
- transferase activity, transferring acyl groups GO:0016746 molecular_function
- InterPro
3VYN
- Name
- L,D-transpeptidase 2 (LDT 2) (EC 2.3.2.-) (Ldt(Mt2))
- Family
- Unknown
- Protein Sequence
-
MPKVGIAAQAGRTRVRRAWLTALMMTAVMIGAVACGSGRGPAPIKVIADKGTPFADLLVPKLTASVTDGAVGVTVDAPVSVTAADGVLAAVTMVNDNGRPVAGRLSPDGLRWSTTEQLGYNRRYTLNATALGLGGAATRQLTFQTSSPAHLTMPYVMPGDGEVVGVGEPVAIRFDENIADRGAAEKAIKITTNPPVEGAFYWLNNREVRWRPEHFWKPGTAVDVAVNTYGVDLGEGMFGEDNVQTHFTIGDEVIATADDNTKILTVRVNGEVVKSMPTSMGKDSTPTANGIYIVGSRYKHIIMDSSTYGVPVNSPNGYRTDVDWATQISYSGVFVHSAPWSVGAQGHTNTSHGCLNVSPSNAQWFYDHVKRGDIVEVVNTVGGTLPGIDGLGDWNIPWDQWRAGNAKA
- Mass
- 43,366 Da
- Length
- 408 Aa
Summary
Rv2518c doesn't seem to be a targeted by any drug.
Rv2518c doesn't seem to be involved in any pathway.
- Crystal structure of L,D-transpeptidase LdtMt2 in complex with meropenem reveals the mechanism of carbapenem against Mycobacterium tuberculosis.
- Cell Res. 2013 May;23(5):728-31. doi: 10.1038/cr.2013.53. Epub 2013 Apr 16.
- Structure of LdtMt2, an L,D-transpeptidase from Mycobacterium tuberculosis.
- Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):432-41. doi: 10.1107/S0907444912049268. Epub 2013 Feb 16.
- Structural basis for the inhibition of Mycobacterium tuberculosis L,D-transpeptidase by meropenem, a drug effective against extensively drug-resistant strains.
- Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):420-31. doi: 10.1107/S0907444912048998. Epub 2013 Feb 16.
- In vitro cross-linking of Mycobacterium tuberculosis peptidoglycan by L,D-transpeptidases and inactivation of these enzymes by carbapenems.
- Antimicrob Agents Chemother. 2013 Dec;57(12):5940-5. doi: 10.1128/AAC.01663-13. Epub 2013 Sep 16.
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.