Gene - combatTB X

relA

Type: protein_coding
Name: relA
Locus Name: Rv2583c
Product: Probable GTP pyrophosphokinase RelA (ATP:GTP 3'-pyrophosphotransferase) (PPGPP synthetase I) ((P)PPGPP synthetase) (GTP diphosphokinase)
Functional Category: Intermediary metabolism and respiration
Location: 2907826..2910198 (- strand)
Gene Length: 2372 bp
Nucleotides: GTGGCCGAGGACCAGCTCACGGCGCAAGCGGTTGCACCGCCCACGGAGGCTTCTGCGGCTCTCGAGCCCGCTCTCGAGACGCCCGAGTCGCCGGTCGAGACTCTTAAGACCAGCATCAGCGCGTCGCGTCGGGTGCGGGCCCGATTGGCCCGGCGGATGACCGCCCAGCGCAGCACCACCAATCCGGTGCTCGAGCCGTTGGTGGCGGTGCACCGGGAGATCTATCCCAAGGCCGACCTGTCGATCTTGCAGCGAGCCTACGAGGTCGCTGACCAAAGGCATGCCAGCCAGTTGCGGCAGTCCGGTGATCCCTACATCACCCACCCGTTGGCCGTTGCCAACATTCTGGCCGAGTTGGGCATGGACACCACCACTTTGGTGGCCGCGCTGCTGCACGACACCGTCGAGGACACCGGTTACACCCTGGAGGCGTTGACCGAGGAATTCGGCGAAGAGGTGGGCCATCTCGTCGACGGGGTGACCAAGCTGGATCGGGTGGTGTTGGGCAGCGCCGCCGAAGGCGAGACTATTCGCAAGATGATCACCGCGATGGCCCGCGATCCGCGGGTGCTGGTGATAAAGGTGGCTGACCGGTTACACAACATGCGCACCATGCGCTTCTTGCCGCCGGAGAAGCAGGCCCGCAAGGCCCGTGAGACGTTGGAAGTCATTGCACCCCTGGCGCATCGGCTGGGCATGGCCAGCGTCAAGTGGGAGTTGGAGGACCTGTCCTTCGCGATCCTGCATCCCAAGAAGTACGAGGAGATCGTCCGGCTGGTCGCCGGTCGCGCGCCGTCCCGGGACACCTACCTGGCCAAGGTGCGTGCCGAAATCGTCAACACGCTGACCGCGTCGAAGATCAAGGCGACGGTGGAGGGCCGCCCCAAGCACTATTGGTCGATCTACCAGAAGATGATCGTTAAGGGCCGCGACTTCGACGACATCCACGACCTGGTCGGTGTGCGCATCCTGTGCGACGAAATCCGGGACTGCTACGCGGCTGTCGGCGTAGTGCATTCGCTATGGCAGCCGATGGCGGGTCGGTTCAAGGACTACATCGCCCAGCCCAGATACGGTGTGTACCAGTCACTGCACACCACTGTGGTCGGGCCTGAGGGCAAGCCGCTGGAAGTGCAGATCCGTACCCGCGACATGCACCGCACCGCCGAATACGGCATCGCCGCGCATTGGCGCTACAAAGAAGCCAAGGGCCGCAACGGTGTTCTTCATCCGCATGCCGCCGCGGAGATCGACGACATGGCCTGGATGCGTCAGCTGCTCGACTGGCAACGTGAGGCGGCCGACCCCGGTGAGTTCTTGGAATCATTGCGCTACGACCTTGCGGTGCAAGAGATTTTCGTGTTTACCCCCAAGGGCGACGTGATCACGCTGCCAACCGGTTCGACGCCGGTGGACTTCGCTTACGCGGTGCACACAGAGGTGGGCCACCGCTGCATCGGCGCCCGAGTGAACGGCCGGTTGGTAGCGCTGGAACGCAAGCTGGAAAACGGAGAAGTTGTCGAGGTTTTCACGTCCAAGGCGCCGAACGCCGGGCCGTCGCGGGACTGGCAGCAGTTCGTGGTGTCGCCGCGCGCAAAGACGAAGATCCGCCAGTGGTTCGCCAAGGAGCGGCGTGAGGAGGCGTTGGAGACCGGTAAGGATGCGATGGCCCGCGAGGTGCGCCGCGGTGGACTTCCGTTGCAGCGCTTGGTCAATGGTGAGTCCATGGCGGCGGTGGCCCGCGAGCTGCACTACGCGGACGTGTCAGCACTCTATACCGCCATCGGTGAGGGGCACGTGTCGGCGAAACACGTCGTGCAGCGGTTGTTGGCCGAGCTCGGCGGTATCGACCAGGCGGAAGAGGAACTCGCCGAGCGGTCCACGCCGGCGACCATGCCGCGGCGCCCACGCAGCACCGACGATGTCGGGGTCTCCGTCCCCGGCGCCCCGGGCGTGCTGACCAAGCTGGCCAAGTGCTGCACGCCGGTTCCGGGCGATGTGATTATGGGGTTCGTCACCCGTGGCGGCGGGGTCAGTGTGCACCGCACCGACTGCACCAACGCCGCATCGCTGCAGCAGCAGGCCGAGCGCATCATCGAGGTGCTATGGGCGCCGTCGCCGTCGTCGGTGTTTCTGGTGGCAATCCAGGTCGAGGCACTCGACCGGCACCGGCTGCTGTCGGATGTGACGCGCGCACTGGCCGACGAGAAGGTCAATATCCTGTCCGCGTCGGTCACCACTTCGGGGGACCGGGTGGCGATCAGTCGATTCACCTTCGAGATGGGTGACCCCAAGCACCTCGGGCACCTGCTCAACGCCGTCCGCAACGTCGAAGGTGTCTACGACGTCTACCGGGTGACCTCGGCCGCGTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the formation of pppGpp, which is then hydrolyzed to form ppGpp, as well as the hydrolysis of ppGpp. RelA is probably a key factor in the pathogenesis of M.tuberculosis as it regulates the intracellular concentrations of (p)ppGpp. {ECO:0000269|PubMed:10375643, ECO:0000269|PubMed:10940033}.

Family

RelA/SpoT family

GO

pathogenesis GO:0009405 biological_process
stringent response GO:0015968 biological_process
kinase activity GO:0016301 molecular_function
guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity GO:0008893 molecular_function
plasma membrane GO:0005886 cellular_component
GTP binding GO:0005525 molecular_function
ATP binding GO:0005524 molecular_function
cell wall GO:0005618 cellular_component
manganese ion binding GO:0030145 molecular_function
GTP diphosphokinase activity GO:0008728 molecular_function
guanosine tetraphosphate biosynthetic process GO:0015970 biological_process

InterPro

UniProt: P9WHG9
GenBank: Rv2583c
EnsemblBacteria: Rv2583c
Mycobrowser: Rv2583c

Summary

Name: Bifunctional (p)ppGpp synthase/hydrolase RelA [Includes: GTP pyrophosphokinase (EC 2.7.6.5) ((p)ppGpp synthase) (ATP:GTP 3'-pyrophosphotransferase) (Stringent response-like protein) (ppGpp synthase II); Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) (Penta-phosphate guanosine-3'-pyrophosphohydrolase) ((ppGpp)ase)]
Family: RelA/SpoT family
Protein Sequence: MTAQRSTTNPVLEPLVAVHREIYPKADLSILQRAYEVADQRHASQLRQSGDPYITHPLAVANILAELGMDTTTLVAALLHDTVEDTGYTLEALTEEFGEEVGHLVDGVTKLDRVVLGSAAEGETIRKMITAMARDPRVLVIKVADRLHNMRTMRFLPPEKQARKARETLEVIAPLAHRLGMASVKWELEDLSFAILHPKKYEEIVRLVAGRAPSRDTYLAKVRAEIVNTLTASKIKATVEGRPKHYWSIYQKMIVKGRDFDDIHDLVGVRILCDEIRDCYAAVGVVHSLWQPMAGRFKDYIAQPRYGVYQSLHTTVVGPEGKPLEVQIRTRDMHRTAEYGIAAHWRYKEAKGRNGVLHPHAAAEIDDMAWMRQLLDWQREAADPGEFLESLRYDLAVQEIFVFTPKGDVITLPTGSTPVDFAYAVHTEVGHRCIGARVNGRLVALERKLENGEVVEVFTSKAPNAGPSRDWQQFVVSPRAKTKIRQWFAKERREEALETGKDAMAREVRRGGLPLQRLVNGESMAAVARELHYADVSALYTAIGEGHVSAKHVVQRLLAELGGIDQAEEELAERSTPATMPRRPRSTDDVGVSVPGAPGVLTKLAKCCTPVPGDVIMGFVTRGGGVSVHRTDCTNAASLQQQAERIIEVLWAPSPSSVFLVAIQVEALDRHRLLSDVTRALADEKVNILSASVTTSGDRVAISRFTFEMGDPKHLGHLLNAVRNVEGVYDVYRVTSAA
Mass: 81,827 Da
Length: 738 Aa

Rv2583c doesn't seem to be a targeted by any drug.

Purine metabolism mtu00230
mtu00230
PATHWAY_MAP mtu00230 Purine metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Mycobacterial MazG is a novel NTP pyrophosphohydrolase involved in oxidative stress response.: J Biol Chem. 2010 Sep 3;285(36):28076-85. doi: 10.1074/jbc.M109.088872. Epub 2010 Jun 7.
Functional regulation of the opposing (p)ppGpp synthetase/hydrolase activities of RelMtb from Mycobacterium tuberculosis.: Biochemistry. 2005 Jul 26;44(29):9913-23. doi: 10.1021/bi0505316.
The stringent response of Mycobacterium tuberculosis is required for long-term survival.: J Bacteriol. 2000 Sep;182(17):4889-98. doi: 10.1128/jb.182.17.4889-4898.2000.
Differential regulation of opposing RelMtb activities by the aminoacylation state of a tRNA.ribosome.mRNA.RelMtb complex.: Biochemistry. 2000 Sep 26;39(38):11640-8. doi: 10.1021/bi001256k.
Cloning and characterization of a bifunctional RelA/SpoT homologue from Mycobacterium tuberculosis.: Gene. 1999 Jun 11;233(1-2):261-9.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.