pimA
- Type
- protein_coding
- Name
- pimA
- Locus Name
-
Rv2610c
- Product
-
Alpha-mannosyltransferase PimA
- Functional Category
-
Lipid metabolism
- Location
- 2937865..2939001 (- strand)
- Gene Length
- 1136 bp
- Nucleotides
-
ATGCGGATCGGCATGATTTGTCCGTACTCGTTCGACGTCCCGGGCGGGGTGCAGTCGCATGTGCTACAGCTTGCCGAGGTGATGCGCACCCGCGGCCACTTGGTCAGTGTGCTCGCGCCGGCCTCCCCGCATGCCGCACTGCCTGATTACTTTGTCTCCGGTGGTAGGGCGGTTCCGATTCCCTACAACGGCTCGGTGGCCCGGCTGCGGTTCGGTCCGGCGACCCACCGCAAGGTCAAAAAGTGGCTTGCGCATGGTGATTTCGACGTACTGCATCTACACGAGCCGAATGCGCCGAGCCTGTCGATGCTGGCCCTGAACATTGCCGAGGGCCCGATTGTGGCGACATTTCACACCTCGACCACCAAGTCGCTGACGCTGACGGTGTTCCAGGGCATTCTGCGGCCCATGCACGAGAAGATCGTCGGCCGGATCGCGGTGTCCGACCTAGCCCGGCGCTGGCAGATGGAGGCGTTGGGATCCGATGCGGTGGAGATCCCCAATGGGGTGGACGTTGATTCCTTCGCCTCGGCAGCGCGGCTGGACGGGTACCCGCGCCAGGGTAAAACGGTGTTGTTCCTGGGTCGCTACGACGAGCCCCGCAAGGGCATGGCCGTTTTGCTCGACGCGCTGCCGAAGGTGGTGCAGCGGTTTCCGGATGTCCAGCTACTGATCGTCGGCCACGGCGACGCCGACCAGTTGCGCGGCCAGGCGGGCCGTTTGGCGGCGCACCTGCGCTTTCTGGGTCAGGTGGACGACGCCGGAAAAGCTTCGGCGATGCGCAGCGCCGACGTCTACTGTGCGCCCAACACCGGCGGTGAGAGTTTCGGCATTGTGCTGGTCGAAGCGATGGCCGCCGGCACTGCGGTGGTGGCCAGCGACCTCGACGCCTTCCGGCGTGTGCTGCGCGACGGTGAGGTCGGGCACCTGGTGCCGGTGGACCCGCCAGACTTGCAGGCCGCCGCGTTGGCCGATGGACTGATTGCGGTGCTGGAGAACGATGTCCTGCGGGAGCGCTATGTGGCGGCCGGCAACGCGGCCGTCCGCCGGTATGACTGGTCGGTGGTGGCCAGCCAGATCATGCGAGTGTACGAGACGGTCGCCGGGTCGGGCGCCAAGGTTCAGGTGGCCAGCTG
- Drug Resistance
- Mutations
- Function
- Involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM). Catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) (PubMed:15939292). PimA plays an essential role for growth in macrophages and during both the acute and chronic phases of infection (PubMed:25049093). {ECO:0000269|PubMed:15939292, ECO:0000269|PubMed:25049093}.
- Family
-
Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily
- GO
-
- phosphatidylinositol metabolic process GO:0046488 biological_process
- pathogenesis GO:0009405 biological_process
- magnesium ion binding GO:0000287 molecular_function
- glycolipid biosynthetic process GO:0009247 biological_process
- phospholipid biosynthetic process GO:0008654 biological_process
- plasma membrane GO:0005886 cellular_component
- mannosyltransferase activity GO:0000030 molecular_function
- GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity GO:0004377 molecular_function
- phosphatidylinositol alpha-mannosyltransferase activity GO:0043750 molecular_function
- InterPro
Summary
- Name
- Phosphatidyl-myo-inositol mannosyltransferase (EC 2.4.1.345) (Alpha-mannosyltransferase) (GDP-mannose-dependent alpha-(1-2)-phosphatidylinositol mannosyltransferase) (Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase) (Phosphatidylinositol alpha-mannosyltransferase) (PI alpha-mannosyltransferase)
- Family
- Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily
- Protein Sequence
-
MRIGMICPYSFDVPGGVQSHVLQLAEVMRTRGHLVSVLAPASPHAALPDYFVSGGRAVPIPYNGSVARLRFGPATHRKVKKWLAHGDFDVLHLHEPNAPSLSMLALNIAEGPIVATFHTSTTKSLTLTVFQGILRPMHEKIVGRIAVSDLARRWQMEALGSDAVEIPNGVDVDSFASAARLDGYPRQGKTVLFLGRYDEPRKGMAVLLDALPKVVQRFPDVQLLIVGHGDADQLRGQAGRLAAHLRFLGQVDDAGKASAMRSADVYCAPNTGGESFGIVLVEAMAAGTAVVASDLDAFRRVLRDGEVGHLVPVDPPDLQAAALADGLIAVLENDVLRERYVAAGNAAVRRYDWSVVASQIMRVYETVAGSGAKVQVAS
- Mass
- 40,445 Da
- Length
- 378 Aa
Rv2610c doesn't seem to be a targeted by any drug.
-
-
Lipoarabinomannan (LAM) biosynthesis mtu00571
mtu00571Metabolism; Glycan biosynthesis and metabolism
-
- The phosphatidyl-myo-inositol mannosyltransferase PimA is essential for Mycobacterium tuberculosis growth in vitro and in vivo.
- J Bacteriol. 2014 Oct;196(19):3441-51. doi: 10.1128/JB.01346-13. Epub 2014 Jul 21.
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.
- BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
- Expression and purification of a functionally active recombinant GDP-mannosyltransferase (PimA) from Mycobacterium tuberculosis H37Rv.
- Protein Expr Purif. 2005 Jul;42(1):47-53. doi: 10.1016/j.pep.2005.03.015. Epub 2005 Apr 5.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.