Gene - combatTB X

dxs1

Type: protein_coding
Name: dxs1
Locus Name: Rv2682c
Product: Probable 1-deoxy-D-xylulose 5-phosphate synthase Dxs1 (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS)
Functional Category: Intermediary metabolism and respiration
Location: 2998052..2999968 (- strand)
Gene Length: 1916 bp
Nucleotides: ATGCTGCAACAGATCCGCGGGCCCGCTGATCTGCAGCACCTTTCCCAGGCGCAGCTTCGGGAGCTGGCCGCCGAGATCCGTGAGTTCCTGATCCACAAGGTTGCCGCCACGGGGGGGCATCTGGGGCCGAACCTGGGAGTGGTGGAACTCACCTTGGCGCTGCACCGGGTATTCGACTCGCCGCACGATCCGATCATCTTCGACACCGGTCACCAGGCCTACGTCCACAAGATGTTGACCGGACGCAGCCAGGACTTCGCAACCCTGCGTAAGAAGGGCGGGTTGTCGGGGTATCCGTCTCGTGCCGAGAGCGAGCACGACTGGGTGGAGTCGAGCCACGCCAGCGCGGCGCTGTCGTACGCGGACGGGTTGGCCAAGGCGTTCGAGTTGACCGGACACCGCAACCGGCATGTGGTCGCGGTGGTCGGTGACGGTGCGCTCACCGGCGGTATGTGCTGGGAGGCGCTGAACAATATCGCCGCATCCCGCCGGCCGGTGATTATCGTGGTCAACGACAATGGGCGCAGCTACGCGCCCACAATCGGGGGCGTCGCCGACCATCTGGCCACGCTGCGGCTGCAGCCGGCCTACGAGCAGGCGCTGGAGACGGGCCGCGACCTGGTGCGCGCGGTGCCGCTTGTCGGCGGTCTGTGGTTTCGATTCCTGCACAGCGTCAAGGCCGGCATCAAGGACTCGCTGTCGCCGCAGTTGCTGTTCACCGACCTCGGGTTGAAGTACGTCGGCCCGGTCGACGGCCATGACGAGCGGGCGGTGGAGGTCGCGCTGCGCAGCGCGCGGCGCTTCGGTGCACCGGTGATCGTGCACGTCGTCACCCGCAAGGGCATGGGCTACCCGCCGGCCGAGGCCGACCAGGCCGAGCAGATGCATTCCACGGTCCCGATCGATCCGGCCACCGGACAAGCCACCAAGGTGGCCGGCCCAGGCTGGACGGCGACCTTCTCTGATGCACTTATCGGCTACGCCCAGAAACGCCGTGACATCGTGGCCATTACCGCGGCCATGCCGGGCCCCACCGGGCTGACCGCGTTCGGGCAGCGCTTCCCGGATCGATTGTTCGACGTCGGGATCGCCGAGCAACACGCGATGACGTCGGCGGCCGGGTTGGCGATGGGTGGGCTGCACCCCGTGGTGGCGATCTACTCGACGTTCCTGAACCGGGCGTTCGACCAGATCATGATGGATGTGGCGCTGCACAAGCTGCCGGTCACCATGGTGCTGGACCGTGCCGGGATCACCGGTAGCGACGGCGCCAGCCACAACGGAATGTGGGACTTGTCGATGCTGGGTATCGTGCCCGGCATCCGGGTGGCAGCGCCCAGAGACGCCACCCGGTTGCGTGAAGAACTCGGCGAGGCGCTCGACGTCGACGACGGCCCGACGGCGTTACGGTTCCCCAAAGGTGATGTGGGAGAAGATATTTCGGCTTTGGAGCGGCGTGGAGGCGTGGATGTGCTGGCGGCGCCCGCCGATGGTTTGAACCACGACGTCCTGTTGGTGGCCATCGGCGCGTTCGCACCGATGGCGTTGGCGGTGGCCAAGCGGCTGCACAACCAGGGGATCGGTGTGACGGTGATCGACCCGCGCTGGGTGTTGCCGGTGTCTGACGGTGTGCGCGAACTGGCGGTGCAGCACAAGCTGCTCGTCACGCTAGAGGACAACGGGGTCAACGGTGGGGCGGGGTCAGCGGTGTCGGCCGCGCTGCGGCGCGCGGAGATCGACGTGCCCTGCCGCGATGTCGGGTTGCCGCAGGAGTTCTACGAGCACGCGTCTCGAAGCGAGGTGCTGGCCGATCTGGGGCTTACCGACCAGGACGTGGCCCGGCGGATCACCGGCTGGGTCGCCGCGCTGGGTACCGGGGTGTGTGCGTCCGACGCGATTCCAGAACATCTCGACTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP). {ECO:0000255|HAMAP-Rule:MF_00315}.

Family

Transketolase family, DXPS subfamily

GO

isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway GO:0019288 biological_process
terpenoid biosynthetic process GO:0016114 biological_process
cytosol GO:0005829 cellular_component
thiamine pyrophosphate binding GO:0030976 molecular_function
thiamine binding GO:0030975 molecular_function
manganese ion binding GO:0030145 molecular_function
1-deoxy-D-xylulose-5-phosphate synthase activity GO:0008661 molecular_function
1-deoxy-D-xylulose 5-phosphate biosynthetic process GO:0052865 biological_process
thiamine biosynthetic process GO:0009228 biological_process
magnesium ion binding GO:0000287 molecular_function
positive regulation of gene expression GO:0010628 biological_process

InterPro

UniProt: P9WNS3
GenBank: Rv2682c
EnsemblBacteria: Rv2682c
Mycobrowser: Rv2682c

Summary

Name: 1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7) (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS)
Family: Transketolase family, DXPS subfamily
Protein Sequence: MLQQIRGPADLQHLSQAQLRELAAEIREFLIHKVAATGGHLGPNLGVVELTLALHRVFDSPHDPIIFDTGHQAYVHKMLTGRSQDFATLRKKGGLSGYPSRAESEHDWVESSHASAALSYADGLAKAFELTGHRNRHVVAVVGDGALTGGMCWEALNNIAASRRPVIIVVNDNGRSYAPTIGGVADHLATLRLQPAYEQALETGRDLVRAVPLVGGLWFRFLHSVKAGIKDSLSPQLLFTDLGLKYVGPVDGHDERAVEVALRSARRFGAPVIVHVVTRKGMGYPPAEADQAEQMHSTVPIDPATGQATKVAGPGWTATFSDALIGYAQKRRDIVAITAAMPGPTGLTAFGQRFPDRLFDVGIAEQHAMTSAAGLAMGGLHPVVAIYSTFLNRAFDQIMMDVALHKLPVTMVLDRAGITGSDGASHNGMWDLSMLGIVPGIRVAAPRDATRLREELGEALDVDDGPTALRFPKGDVGEDISALERRGGVDVLAAPADGLNHDVLLVAIGAFAPMALAVAKRLHNQGIGVTVIDPRWVLPVSDGVRELAVQHKLLVTLEDNGVNGGAGSAVSAALRRAEIDVPCRDVGLPQEFYEHASRSEVLADLGLTDQDVARRITGWVAALGTGVCASDAIPEHLD
Mass: 67,885 Da
Length: 638 Aa

Rv2682c doesn't seem to be a targeted by any drug.

Terpenoid backbone biosynthesis mtu00900
mtu00900
Metabolism; Metabolism of terpenoids and polyketides

Thiamine metabolism mtu00730
mtu00730
PATHWAY_MAP mtu00730 Thiamine metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.