Gene - combatTB X

dut

Type: protein_coding
Name: dut
Locus Name: Rv2697c
Product: Probable deoxyuridine 5'-triphosphate nucleotidohydrolase Dut (dUTPase) (dUTP pyrophosphatase) (deoxyuridine 5'-triphosphatase) (dUTP diphosphatase) (deoxyuridine-triphosphatase)
Functional Category: Intermediary metabolism and respiration
Location: 3013683..3014147 (- strand)
Gene Length: 464 bp
Nucleotides: GTGTCGACCACTCTGGCGATCGTCCGCCTCGACCCCGGGCTCCCGCTGCCCAGCCGCGCTCACGACGGCGACGCCGGCGTTGATCTCTACAGCGCCGAAGACGTCGAGCTGGCACCTGGGCGCCGCGCCCTGGTACGGACGGGTGTTGCGGTCGCCGTCCCGTTCGGCATGGTCGGGCTGGTCCATCCGCGCTCCGGGTTGGCCACGCGGGTGGGGCTTTCGATCGTCAACAGTCCGGGCACCATCGACGCGGGTTATCGTGGGGAGATCAAGGTGGCCCTGATCAACTTGGACCCAGCCGCGCCCATCGTGGTACATCGCGGTGACCGAATCGCCCAGTTGCTAGTGCAACGGGTTGAGTTGGTCGAGCTGGTCGAGGTCTCGTCGTTCGACGAGGCCGGGCTGGCCTCGACATCCCGCGGCGACGGTGGCCACGGTTCCTCCGGCGGACATGCGAGTTTGTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Family

DUTPase family

GO

dUTP diphosphatase activity GO:0004170 molecular_function
dUMP biosynthetic process GO:0006226 biological_process
magnesium ion binding GO:0000287 molecular_function
dUTP metabolic process GO:0046080 biological_process
dUTP catabolic process GO:0046081 biological_process

InterPro

UniProt: P9WNS5
GenBank: Rv2697c
EnsemblBacteria: Rv2697c
Mycobrowser: Rv2697c

PDB IDs:

Summary

Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) (EC 3.6.1.23) (dUTP pyrophosphatase)
Family: DUTPase family
Protein Sequence: MSTTLAIVRLDPGLPLPSRAHDGDAGVDLYSAEDVELAPGRRALVRTGVAVAVPFGMVGLVHPRSGLATRVGLSIVNSPGTIDAGYRGEIKVALINLDPAAPIVVHRGDRIAQLLVQRVELVELVEVSSFDEAGLASTSRGDGGHGSSGGHASL
Mass: 15,803 Da
Length: 154 Aa

Rv2697c doesn't seem to be a targeted by any drug.

Rv2697c doesn't seem to be involved in any pathway.

Crystal structure of the Mycobacterium tuberculosis dUTPase: insights into the catalytic mechanism.: J Mol Biol. 2004 Aug 6;341(2):503-17. doi: 10.1016/j.jmb.2004.06.028.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.