Gene - combatTB X

recA

Type: protein_coding
Name: recA
Locus Name: Rv2737c
Product: RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)].
Functional Category: Information pathways
Location: 3049052..3051424 (- strand)
Gene Length: 2372 bp
Nucleotides: ATGACGCAGACCCCCGATCGGGAAAAGGCGCTCGAGCTGGCAGTGGCCCAGATCGAGAAGAGTTACGGCAAAGGTTCGGTGATGCGCCTCGGCGACGAGGCGCGTCAGCCGATTTCGGTCATTCCGACCGGATCCATCGCACTAGACGTGGCCCTGGGCATTGGCGGCCTGCCGCGTGGCCGGGTGATAGAGATATACGGCCCGGAGTCGTCGGGTAAGACCACCGTGGCGCTGCACGCGGTGGCCAACGCTCAGGCCGCCGGTGGTGTTGCGGCGTTCATCGACGCCGAGCACGCGCTGGATCCGGACTATGCCAAGAAGCTCGGTGTCGACACCGATTCGCTGCTGGTCAGCCAGCCGGACACCGGGGAACAGGCACTCGAGATCGCCGACATGCTGATCCGCTCGGGTGCGCTTGACATCGTGGTGATCGACTCGGTGGCGGCGCTGGTGCCGCGCGCGGAGCTCGAAGGCGAGATGGGCGACAGCCACGTCGGGCTGCAGGCCCGGCTGATGAGCCAGGCGCTGCGGAAAATGACCGGCGCGCTGAATAATTCGGGCACCACGGCGATCTTCATCAACCAGCTCCGCGACAAGATCGGAGTGATGTTCGGGTCGCCCGAGACGACAACGGGCGGAAAGGCGTTGAAGTTCTACGCGTCGGTGCGCATGGACGTGCGGCGAGTCGAGACGCTCAAGGACGGTACCAACGCGGTCGGCAACCGCACCCGGGTCAAGGTCGTCAAGAACAAGTGCCTCGCAGAGGGCACTCGGATCTTCGATCCGGTCACCGGTACAACGCATCGCATCGAGGATGTTGTCGATGGGCGCAAGCCTATTCATGTCGTGGCTGCTGCCAAGGACGGAACGCTGCATGCGCGGCCCGTGGTGTCCTGGTTCGACCAGGGAACGCGGGATGTGATCGGGTTGCGGATCGCCGGTGGCGCCATCGTGTGGGCGACACCCGATCACAAGGTGCTGACAGAGTACGGCTGGCGTGCCGCCGGGGAACTCCGCAAGGGAGACAGGGTGGCGCAACCGCGACGCTTCGATGGATTCGGTGACAGTGCGCCGATTCCGGCGGATCATGCCCGGCTGCTTGGCTACCTGATCGGAGATGGCAGGGATGGTTGGGTGGGGGGCAAGACTCCGATCAACTTCATCAATGTTCAGCGGGCGCTCATTGACGACGTGACGCGAATCGCTGCGACGCTCGGTTGCGCGGCCCATCCGCAGGGGCGTATCTCACTCGCGATCGCTCATCGACCCGGTGAGCGCAACGGTGTGGCAGACCTTTGTCAGCAGGCCGGTATCTACGGCAAGCTCGCGTGGGAGAAGACGATTCCGAATTGGTTCTTCGAGCCGGACATCGCGGCCGACATTGTCGGCAATCTGCTCTTCGGCCTGTTCGAAAGCGACGGGTGGGTGAGCCGGGAACAGACCGGGGCACTTCGGGTCGGTTACACGACGACCTCTGAACAACTCGCGCATCAGATTCATTGGCTGCTGCTGCGGTTCGGTGTCGGGAGCACCGTTCGAGATTACGATCCGACCCAGAAGCGGCCGAGCATCGTCAACGGTCGACGGATCCAGAGCAAACGTCAAGTGTTCGAGGTCCGGATCTCGGGTATGGATAACGTCACGGCATTCGCGGAGTCAGTTCCCATGTGGGGGCCGCGCGGTGCCGCGCTTATCCAGGCGATTCCAGAAGCCACGCAGGGGCGGCGTCGTGGATCGCAAGCGACATATCTGGCTGCAGAGATGACCGATGCCGTGCTGAATTATCTGGACGAGCGCGGCGTGACCGCGCAGGAGGCCGCGGCCATGATCGGTGTAGCTTCCGGGGACCCCCGCGGTGGAATGAAGCAGGTCTTAGGTGCCAGCCGCCTTCGTCGGGATCGCGTGCAGGCGCTCGCGGATGCCCTGGATGACAAATTCCTGCACGACATGCTGGCGGAAGAACTCCGCTATTCCGTGATCCGAGAAGTGCTGCCAACGCGGCGGGCACGAACGTTCGACCTCGAGGTCGAGGAACTGCACACCCTCGTCGCCGAAGGGGTTGTCGTGCACAACTGTTCGCCCCCCTTCAAGCAGGCCGAGTTCGACATCCTCTACGGCAAGGGAATCAGCAGGGAGGGCTCGCTGATCGACATGGGTGTGGATCAGGGCCTCATCCGCAAGTCGGGTGCCTGGTTCACCTACGAGGGCGAGCAGCTCGGCCAGGGCAAGGAGAATGCCCGCAACTTCTTGGTGGAGAACGCCGACGTGGCTGACGAGATCGAGAAGAAGATCAAGGAAAAGCTTGGCATTGGTGCCGTGGTGACCGATGATCCCTCAAATGACGGTGTCCTGCCCGCCCCCGTCGACTTCTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.; FUNCTION: PI-MtuI is an endonuclease.

Family

RecA family

GO

SOS response GO:0009432 biological_process
response to antibiotic GO:0046677 biological_process
UV protection GO:0009650 biological_process
recombinational repair GO:0000725 biological_process
magnesium ion binding GO:0000287 molecular_function
strand invasion GO:0042148 biological_process
manganese ion binding GO:0030145 molecular_function
ATP binding GO:0005524 molecular_function
cytosol GO:0005829 cellular_component
damaged DNA binding GO:0003684 molecular_function
single-stranded DNA binding GO:0003697 molecular_function
intron homing GO:0006314 biological_process
DNA-dependent ATPase activity GO:0008094 molecular_function
ATPase activity GO:0016887 molecular_function
recombinase activity GO:0000150 molecular_function
cellular response to DNA damage stimulus GO:0006974 biological_process
intein-mediated protein splicing GO:0016539 biological_process
endodeoxyribonuclease activity GO:0004520 molecular_function

InterPro

UniProt: P9WHJ3
GenBank: Rv2737c
EnsemblBacteria: Rv2737c
Mycobrowser: Rv2737c

PDB IDs:

Summary

Name: Protein RecA (Recombinase A) [Cleaved into: Endonuclease PI-MtuI (EC 3.1.-.-) (Mtu RecA intein)]
Family: RecA family
Protein Sequence: MTQTPDREKALELAVAQIEKSYGKGSVMRLGDEARQPISVIPTGSIALDVALGIGGLPRGRVIEIYGPESSGKTTVALHAVANAQAAGGVAAFIDAEHALDPDYAKKLGVDTDSLLVSQPDTGEQALEIADMLIRSGALDIVVIDSVAALVPRAELEGEMGDSHVGLQARLMSQALRKMTGALNNSGTTAIFINQLRDKIGVMFGSPETTTGGKALKFYASVRMDVRRVETLKDGTNAVGNRTRVKVVKNKCLAEGTRIFDPVTGTTHRIEDVVDGRKPIHVVAAAKDGTLHARPVVSWFDQGTRDVIGLRIAGGAIVWATPDHKVLTEYGWRAAGELRKGDRVAQPRRFDGFGDSAPIPADHARLLGYLIGDGRDGWVGGKTPINFINVQRALIDDVTRIAATLGCAAHPQGRISLAIAHRPGERNGVADLCQQAGIYGKLAWEKTIPNWFFEPDIAADIVGNLLFGLFESDGWVSREQTGALRVGYTTTSEQLAHQIHWLLLRFGVGSTVRDYDPTQKRPSIVNGRRIQSKRQVFEVRISGMDNVTAFAESVPMWGPRGAALIQAIPEATQGRRRGSQATYLAAEMTDAVLNYLDERGVTAQEAAAMIGVASGDPRGGMKQVLGASRLRRDRVQALADALDDKFLHDMLAEELRYSVIREVLPTRRARTFDLEVEELHTLVAEGVVVHNCSPPFKQAEFDILYGKGISREGSLIDMGVDQGLIRKSGAWFTYEGEQLGQGKENARNFLVENADVADEIEKKIKEKLGIGAVVTDDPSNDGVLPAPVDF
Mass: 85,389 Da
Length: 790 Aa

Rv2737c doesn't seem to be a targeted by any drug.

Homologous recombination mtu03440
mtu03440
Genetic Information Processing; Replication and repair

Crystal structures of Mycobacterium tuberculosis RecA and its complex with ADP-AlF(4): implications for decreased ATPase activity and molecular aggregation.: Nucleic Acids Res. 2000 Dec 15;28(24):4964-73. doi: 10.1093/nar/28.24.4964.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis [corrected] .: PLoS One. 2010 Jan 6;5(1):e8589. doi: 10.1371/journal.pone.0008589.
Mycobacterium tuberculosis UvrD1 and UvrA proteins suppress DNA strand exchange promoted by cognate and noncognate RecA proteins.: Biochemistry. 2010 Jun 15;49(23):4872-83. doi: 10.1021/bi902021d.
Functional characterization of the precursor and spliced forms of RecA protein of Mycobacterium tuberculosis.: Biochemistry. 1996 Feb 13;35(6):1793-802. doi: 10.1021/bi9517751.
Protein splicing in the maturation of M. tuberculosis recA protein: a mechanism for tolerating a novel class of intervening sequence.: Cell. 1992 Oct 16;71(2):201-10.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product.: J Bacteriol. 1991 Sep;173(18):5653-62. doi: 10.1128/jb.173.18.5653-5662.1991.