Gene - combatTB X

gcpE

Type: protein_coding
Name: gcpE
Locus Name: Rv2868c
Product: Probable GcpE protein
Functional Category: Conserved hypotheticals
Location: 3179368..3180531 (- strand)
Gene Length: 1163 bp
Nucleotides: GTGACTGTAGGCTTGGGCATGCCGCAGCCCCCGGCACCCACGCTCGCTCCCCGGCGCGCCACCCGTCAGCTGATGGTCGGCAACGTCGGCGTGGGCAGTGACCATCCGGTCTCGGTGCAATCGATGTGCACCACCAAAACCCACGACGTCAACTCGACATTGCAACAAATCGCCGAGCTGACCGCGGCCGGATGCGACATCGTGCGGGTGGCCTGCCCGCGCCAGGAGGACGCCGACGCGCTGGCCGAGATCGCCCGGCACAGCCAGATCCCGGTAGTCGCGGACATACATTTCCAGCCGCGCTACATATTCGCCGCCATCGACGCTGGATGTGCCGCGGTGCGGGTCAACCCGGGCAACATCAAGGAGTTTGACGGCCGGGTGGGTGAGGTCGCCAAGGCGGCGGGTGCGGCCGGGATCCCGATCCGAATCGGTGTCAACGCCGGTTCGCTGGACAAACGGTTCATGGAGAAGTATGGCAAAGCCACGCCCGAGGCGCTGGTTGAGTCGGCGCTGTGGGAGGCTTCGCTTTTCGAGGAGCATGGCTTCGGTGACATCAAGATCAGCGTCAAGCACAACGACCCGGTGGTGATGGTCGCCGCCTACGAGCTGCTTGCTGCACGGTGCGACTACCCACTGCACCTCGGTGTCACCGAGGCCGGCCCTGCTTTCCAGGGCACCATCAAGTCCGCGGTTGCCTTCGGCGCGTTGCTGTCGCGGGGCATAGGCGACACCATCCGGGTGTCGTTGTCGGCCCCGCCGGTCGAGGAAGTCAAGGTGGGCAATCAGGTTCTCGAGTCGTTGAACCTGCGGCCGCGTTCGCTCGAGATCGTGTCTTGCCCGTCGTGCGGTCGCGCGCAAGTCGACGTCTACACCCTGGCCAACGAGGTAACCGCCGGCCTGGATGGTCTCGATGTGCCGTTGCGGGTGGCCGTGATGGGGTGTGTCGTCAATGGTCCGGGTGAAGCACGTGAGGCCGACCTGGGCGTGGCGTCCGGCAACGGCAAAGGTCAGATCTTTGTACGGGGCGAAGTGATCAAGACCGTGCCCGAAGCACAGATCGTCGAGACGCTGATCGAGGAGGCGATGCGGCTGGCCGCCGAAATGGGCGAGCAAGATCCGGGCGCGACACCGAGCGGTTCGCCTATTGTGACCGTAAGCTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. {ECO:0000255|HAMAP-Rule:MF_00159}.

Family

IspG family

GO

isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway GO:0019288 biological_process
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity GO:0046429 molecular_function
cell wall GO:0005618 cellular_component
iron ion binding GO:0005506 molecular_function
plasma membrane GO:0005886 cellular_component
4 iron, 4 sulfur cluster binding GO:0051539 molecular_function
terpenoid biosynthetic process GO:0016114 biological_process

InterPro

UniProt: P9WKG3
GenBank: Rv2868c
EnsemblBacteria: Rv2868c
Mycobrowser: Rv2868c

Summary

Name: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (EC 1.17.7.3) (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase)
Family: IspG family
Protein Sequence: MTVGLGMPQPPAPTLAPRRATRQLMVGNVGVGSDHPVSVQSMCTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADALAEIARHSQIPVVADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAGAAGIPIRIGVNAGSLDKRFMEKYGKATPEALVESALWEASLFEEHGFGDIKISVKHNDPVVMVAAYELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRGIGDTIRVSLSAPPVEEVKVGNQVLESLNLRPRSLEIVSCPSCGRAQVDVYTLANEVTAGLDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGEVIKTVPEAQIVETLIEEAMRLAAEMGEQDPGATPSGSPIVTVS
Mass: 40,482 Da
Length: 387 Aa

Rv2868c doesn't seem to be a targeted by any drug.

Terpenoid backbone biosynthesis mtu00900
mtu00900
Metabolism; Metabolism of terpenoids and polyketides

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.