Gene - combatTB X

glnD

Type: protein_coding
Name: glnD
Locus Name: Rv2918c
Product: Probable [protein-PII] uridylyltransferase GlnD (PII uridylyl-transferase) (uridylyl removing enzyme) (UTASE)
Functional Category: Intermediary metabolism and respiration
Location: 3228254..3230680 (- strand)
Gene Length: 2426 bp
Nucleotides: ATGGAGGCAGAAAGTCCTTGTGCCGCAAGCGATTTAGCTGTCGCCCGACGCGAGCTGCTGTCCGGCAACCATCGCGAGCTGGATCCGGTCGGGCTGCGGCAGACGTGGCTGGATCTGCATGAGTCTTGGCTGATCGACAAGGCCGACGAGATCGGGATCGCCGATGCCAGTGGTTTTGCAATCGTCGGGGTCGGCGGGCTCGGCCGCCGCGAGCTGCTGCCGTATTCGGACCTGGACGTGTTGCTGTTGCACGATGGCAAGCCTGCTGACATCTTGCGGCCCGTCGCCGACAGGTTGTGGTATCCGTTGTGGGATGCCAACATTCGGCTCGATCACAGTGTGCGAACGGTTAGTGAGGCATTGACCATCGCCAATTCCGATCTGATGGCCGCTCTAGGCATGCTGGAAGCCCGCCACATCGCCGGCGATCAGCAGCTCTCGTTCGCGTTGATCGACGGCGTGCGACGCCAGTGGCGCAACGGAATTCGTTCCCGCATGGGCGAACTCGTCGAAATGACATATGCGCGTTGGCGGCGCTGTGGCCGGATCGCGCAGCGCGCAGAGCCCGATCTCAAATTGGGTCGCGGCGGCCTTCGGGACGTCCAGTTGCTAGACGCGCTGGCTCTCGCCCAGCTCATCGACCGGCACGGTATCGGCCACACGGACCTGCCGGCGGGTTCGCTGGACGGTGCGTATCGCACCTTGCTGGATGTGCGCACCGAACTGCACCGGGTGTCGGGCCGCGGACGCGACCACCTGCTGGCCCAGTTCGCCGACGAGATCAGCGCCGCGTTGGGTTTCGGTGACCGATTCGATTTGGCGCGCACACTGTCGAGTGCTGGCCGCACCATCGGTTACCACGCCGAAGCCGGGCTGCGGACTGCGGCGAATGCATTGCCGCGGCGCGGCATCTCGGCCTTGGTGCGGCGGCCAAAGCGGCGACCGCTCGACGAGGGTGTCGTCGAGTATGCCGGCGAAATCGTGCTCGCCCGCGACGCCGAACCCGAACACGATCCCGGCCTGGTGCTCCGAGTGGCCGCCGCGTCGGCCGACACCGGATTGCCCATTGGCGCCGCCACCCTGAGCCGGTTGGCCGCCAGCGTCCCGGATCTGCCGACTCCCTGGCCGCAAGAGGCATTGGACGACTTACTAGTTGTGCTCTCCGCCGGCCCCACCACGGTGGCTACCATCGAAGCGCTCGACCGAACCGGGCTCTGGGGCCGGTTGTTGCCGGAATGGGAGCCCATTCGCGACCTTCCGCCGCGCGACGTCGCCCACAAGTGGACGGTTGACCGACACGTGGTCGAGACCGCGGTGCATGCGGCGCCACTGGCCACCCGGGTGGCACGTCCCGACCTGCTTGCGCTTGGCGCGCTGCTGCACGACATCGGCAAGGGACGGGGCACCGATCACAGTGTGCTCGGGGCCGAATTGGTAATCCCGGTTTGCACCAGGCTGGGGTTGTCGCCGCCGGACGTGCGGACGCTCTCCAAGCTGGTCCGCCACCACCTGCTGCTGCCGATTACGGCAACCCGACGTGACTTAAACGACCCCAAAACCATCGAGGCGGTGTCCGAGGCGCTGGGTGGGGATCCGCAACTGCTCGAAGTTTTGCACGCCCTGTCGGAGGCGGACTCGAAGGCCACCGGCCCCGGGGTGTGGAGCGATTGGAAGGCGTCACTGGTCGATGACCTGGTGCGTCGCTGCCGGATGGTGATGGCCGGAGAGTCGCTGCCTCAAGCCGAACCGACTGCACCCCATTATCTTTCGCTGGCGGCCGACCACGGGGTGCATGTGGAGATTAGTCCGCGCGACGGTGAACGCATCGACGCCGTAATAGTGGCGCCGGACGAGCGGGGACTGGTGTCGAAAGCCGCCGCGGTGCTGGCGCTGAACTCGCTGCGCGTACATTCGGCGTCGGTCAACGTCCACCAGGGTGTCGCGATAACCGAGTTTGTGGTGTCACCACTGTTTGGGTCCCCGCCCGCGGCGGAGTTGGTGCGTCAGCAGTTTGTCGGTGCCCTCAACGGCGACGTCGACGTGCTGGGCATGCTGCAGAAGCGGGACAGCGATGCCGCCAGCTTGGTATCCGCTCGGGCCGGGGACGTGCAGGCCGGGGTGCCCGTGACTCGTACGGCCGCTCCGCCCCGCATCCTGTGGCTCGACACCGCCGCGCCGGCCAAGCTGATCCTCGAAGTTCGGGCCATGGATCGGGCGGGTTTGCTCGCGTTGCTGGCCGGGGCGCTGGAGGGCGCGGGAGCCGGCATTGTCTGGGCGAAGGTCAATACGTTCGGATCGACCGCGGCCGATGTGTTCTGTGTGACGGTCCCCGCGGAGTTGGACGCGAGAGCCGCGGTCGAGCAACACCTGCTGGAGGTGTTGGGCGCCAGCGTCGACGTTGTGGTCGACGAACCCGTCGGCGATTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Modifies, by uridylylation and deuridylylation, the PII regulatory protein (GlnB), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII protein and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII protein, and plays an important role in the regulation of nitrogen assimilation and metabolism (Probable). {ECO:0000305|PubMed:17303474}.

Family

GlnD family

GO

[protein-PII] uridylyltransferase activity GO:0008773 molecular_function
adenylyltransferase activity GO:0070566 molecular_function
cell wall GO:0005618 cellular_component
plasma membrane GO:0005886 cellular_component
phosphoric diester hydrolase activity GO:0008081 molecular_function
regulation of nitrogen utilization GO:0006808 biological_process
nitrogen compound metabolic process GO:0006807 biological_process

InterPro

UniProt: P9WN29
GenBank: Rv2918c
EnsemblBacteria: Rv2918c
Mycobrowser: Rv2918c

Summary

Name: Bifunctional uridylyltransferase/uridylyl-removing enzyme (UTase/UR) (Bifunctional [protein-PII] modification enzyme) (Bifunctional nitrogen sensor protein) [Includes: [Protein-PII] uridylyltransferase (PII uridylyltransferase) (UTase) (EC 2.7.7.59); [Protein-PII]-UMP uridylyl-removing enzyme (UR) (EC 3.1.4.-)]
Family: GlnD family
Protein Sequence: MEAESPCAASDLAVARRELLSGNHRELDPVGLRQTWLDLHESWLIDKADEIGIADASGFAIVGVGGLGRRELLPYSDLDVLLLHDGKPADILRPVADRLWYPLWDANIRLDHSVRTVSEALTIANSDLMAALGMLEARHIAGDQQLSFALIDGVRRQWRNGIRSRMGELVEMTYARWRRCGRIAQRAEPDLKLGRGGLRDVQLLDALALAQLIDRHGIGHTDLPAGSLDGAYRTLLDVRTELHRVSGRGRDHLLAQFADEISAALGFGDRFDLARTLSSAGRTIGYHAEAGLRTAANALPRRGISALVRRPKRRPLDEGVVEYAGEIVLARDAEPEHDPGLVLRVAAASADTGLPIGAATLSRLAASVPDLPTPWPQEALDDLLVVLSAGPTTVATIEALDRTGLWGRLLPEWEPIRDLPPRDVAHKWTVDRHVVETAVHAAPLATRVARPDLLALGALLHDIGKGRGTDHSVLGAELVIPVCTRLGLSPPDVRTLSKLVRHHLLLPITATRRDLNDPKTIEAVSEALGGDPQLLEVLHALSEADSKATGPGVWSDWKASLVDDLVRRCRMVMAGESLPQAEPTAPHYLSLAADHGVHVEISPRDGERIDAVIVAPDERGLVSKAAAVLALNSLRVHSASVNVHQGVAITEFVVSPLFGSPPAAELVRQQFVGALNGDVDVLGMLQKRDSDAASLVSARAGDVQAGVPVTRTAAPPRILWLDTAAPAKLILEVRAMDRAGLLALLAGALEGAGAGIVWAKVNTFGSTAADVFCVTVPAELDARAAVEQHLLEVLGASVDVVVDEPVGD
Mass: 86,438 Da
Length: 808 Aa

Rv2918c doesn't seem to be a targeted by any drug.

Two-component system mtu02020
mtu02020
Environmental Information Processing; Signal transduction

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
The role of GlnD in ammonia assimilation in Mycobacterium tuberculosis.: Tuberculosis (Edinb). 2007 Jul;87(4):384-90. doi: 10.1016/j.tube.2006.12.003. Epub 2007 Feb 15.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.